Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_2792 and Pf1N1B4_2793 are separated by 130 nucleotidesPf1N1B4_2793 and Pf1N1B4_2794 are separated by 143 nucleotidesPf1N1B4_2794 and Pf1N1B4_2795 are separated by 205 nucleotidesPf1N1B4_2795 and Pf1N1B4_2796 are separated by 154 nucleotides Pf1N1B4_2792 - FIG00953454: hypothetical protein, at 3,137,805 to 3,138,101 _2792 Pf1N1B4_2793 - FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8), at 3,138,232 to 3,138,849 _2793 Pf1N1B4_2794 - FIG00953595: hypothetical protein, at 3,138,993 to 3,139,250 _2794 Pf1N1B4_2795 - Alkylphosphonate utilization operon protein PhnA, at 3,139,456 to 3,139,797 _2795 Pf1N1B4_2796 - Octaprenyl diphosphate synthase (EC 2.5.1.90) / Dimethylallyltransferase (EC 2.5.1.1) / (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10) / Geranylgeranyl diphosphate synthase (EC 2.5.1.29), at 3,139,952 to 3,140,920 _2796
Group Condition Pf1N1B4_2792 Pf1N1B4_2793 Pf1N1B4_2794 Pf1N1B4_2795 Pf1N1B4_2796
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.8 -0.8 -0.5 -0.4 N.D.
stress Fusidic 0.125 mg/ml -1.0 -0.7 +0.1 -0.7 N.D.
nitrogen source nitrogen source nitrite 0.1mM -1.2 -0.1 -1.1 +0.2 N.D.
carbon source carbon source Sucrose 0.25mM +0.4 -0.4 -0.4 -1.6 N.D.
anaerobic N2E2wt with N1B4m denitrifying repA -0.1 +0.3 -1.3 -0.9 N.D.
temperature LB 37C -1.3 -0.2 -0.2 -0.3 N.D.
carbon source D-Ribose (C) -0.5 +0.1 -1.2 -0.2 N.D.
stress D-Cycloserine 0.1 mg/ml -0.3 -0.5 -0.2 -0.8 N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A -1.3 +0.2 -0.2 -0.4 N.D.
carbon source carbon source Sucrose 1mM +0.1 +0.9 -1.9 -0.5 N.D.
carbon source carbon source Sucrose 20mM +0.4 +0.1 -1.9 +0.2 N.D.
anaerobic Nitrate reduction +0.3 -0.1 -1.1 -0.2 N.D.
stress Fusidic 0.0625 mg/ml -0.6 +0.6 -0.4 -0.7 N.D.
nitrogen source Adenine (N) -0.4 +0.4 -0.9 -0.2 N.D.
carbon source L-Alanine (C) -0.7 -0.1 +0.6 -0.6 N.D.
stress Spectinomycin 0.05 mg/ml -0.2 -0.3 +0.7 -0.9 N.D.
stress Nalidixic 0.004 mg/ml -0.8 +0.9 -0.5 -0.2 N.D.
nitrogen source L-Isoleucine (N) -0.2 -0.8 +0.6 -0.2 N.D.
temperature LB 37C -0.6 -0.6 +0.1 +0.7 N.D.
stress acetate 20 mM +0.2 +0.5 -1.0 -0.0 N.D.
carbon source carbon source Sucrose 1mM +0.9 -0.3 -1.1 +0.6 N.D.
carbon source octanoate (C) -0.8 +0.3 +0.8 -0.3 N.D.
stress Chloride 200 mM -0.8 +0.8 +0.2 +0.0 N.D.
stress Choline Lysine 6 mM -0.3 +0.9 -0.4 -0.1 N.D.
stress Carbenicillin 2 mg/ml -0.6 +1.0 -0.3 +0.1 N.D.
carbon source carbon source Sucrose 0.25mM -1.1 +0.6 +0.9 -0.2 N.D.
nitrogen source nitrogen source nitrate 20mM -0.3 +0.8 +0.3 -0.5 N.D.
carbon source carbon source Sucrose 0.5mM -0.3 +0.6 +1.1 -0.8 N.D.
stress Choline chloride 200 mM -1.0 +1.0 +0.3 +0.4 N.D.
stress Chloride 300 mM -0.4 +1.0 +0.4 +0.4 N.D.
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