Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_2760 and Pf1N1B4_2761 are separated by 3 nucleotidesPf1N1B4_2761 and Pf1N1B4_2762 are separated by 48 nucleotidesPf1N1B4_2762 and Pf1N1B4_2763 are separated by 71 nucleotidesPf1N1B4_2763 and Pf1N1B4_2764 are separated by 1190 nucleotides Pf1N1B4_2760 - putative Cytochrome bd2, subunit I, at 3,098,426 to 3,099,868 _2760 Pf1N1B4_2761 - putative Cytochrome bd2, subunit II, at 3,099,872 to 3,100,879 _2761 Pf1N1B4_2762 - Cyanide insensitive terminal oxidase, putative subunit III, at 3,100,928 to 3,101,098 _2762 Pf1N1B4_2763 - 23S rRNA (guanine-N-2-) -methyltransferase rlmG (EC 2.1.1.-), at 3,101,170 to 3,102,294 _2763 Pf1N1B4_2764 - Ferredoxin--NADP(+) reductase (EC 1.18.1.2), at 3,103,485 to 3,104,261 _2764
Group Condition Pf1N1B4_2760 Pf1N1B4_2761 Pf1N1B4_2762 Pf1N1B4_2763 Pf1N1B4_2764
stress Aluminum chloride 2.5 mM +0.2 -0.5 N.D. -1.0 -1.0
stress Thallium(I) acetate 0.008 mg/ml +0.2 +0.2 N.D. +0.5 -2.7
stress Chlorite 1 mM -0.7 -0.9 N.D. -0.1 -0.1
stress Paraquat dichloride 0.016 mg/mL +0.1 +0.0 N.D. -0.1 -1.8
stress Thallium(I) acetate 0.008 mg/ml +0.2 +0.2 N.D. +0.1 -1.7
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 +0.1 -0.5 N.D. +0.0 -0.7
stress Fusidic 0.125 mg/ml +0.1 -0.5 N.D. -0.5 -0.3
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A -0.2 -0.7 N.D. +0.3 -0.3
carbon source Glycerol (C) -0.1 -0.4 N.D. -0.8 +0.4
carbon source Uridine (C) +0.2 -0.1 N.D. -0.9 +0.1
carbon source carbon source Sucrose 0.25mM +0.2 +0.7 N.D. -1.1 -0.3
carbon source Cytidine (C) +0.1 -0.0 N.D. -0.8 +0.4
carbon source carbon source Sucrose 20mM -0.1 -0.4 N.D. +0.5 +0.3
nitrogen source nitrogen source nitrate 20mM -0.1 -0.4 N.D. +0.7 +0.0
nitrogen source nitrogen source nitrite 0.1mM +0.1 -0.4 N.D. +0.9 -0.1
carbon source octanoate (C) +0.2 +0.2 N.D. -0.4 +0.3
carbon source carbon source Sucrose 1mM +0.6 +0.4 N.D. -0.6 +0.1
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.2 -0.1 N.D. +0.6 +0.5
carbon source m-Inositol (C) +0.3 +0.3 N.D. -0.3 +0.4
temperature LB 37C -0.3 +0.1 N.D. +0.5 +0.5
carbon source D-Glucose (C) -0.3 +0.1 N.D. +0.4 +0.7
denitrifying Nitrate reduction, replicate C t2 +0.2 +0.2 N.D. +0.7 -0.1
stress Carbenicillin 2 mg/ml +0.2 -0.1 N.D. +0.2 +0.7
carbon source carbon source Sucrose 1mM +0.4 +0.1 N.D. +0.9 -0.4
stress Cisplatin 0.006 mg/ml +0.1 +0.1 N.D. +1.0 +0.1
stress Doxycycline hyclate 0.0001 mg/ml +0.3 -0.1 N.D. +0.2 +0.9
carbon source D-Galactose (C) +0.1 +0.1 N.D. +0.6 +0.4
stress Cholin acetate 20 mM +0.2 +0.0 N.D. +0.3 +0.8
stress Sisomicin 0.001 mg/ml +0.5 +0.1 N.D. +0.3 +0.6
carbon source carbon source Sucrose 0.25mM +0.2 +0.4 N.D. +0.4 +0.5
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