Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_2726 and Pf1N1B4_2727 are separated by 30 nucleotidesPf1N1B4_2727 and Pf1N1B4_2728 are separated by 97 nucleotidesPf1N1B4_2728 and Pf1N1B4_2729 are separated by 141 nucleotidesPf1N1B4_2729 and Pf1N1B4_2730 are separated by 88 nucleotides Pf1N1B4_2726 - Cobalt-zinc-cadmium resistance protein, at 3,064,406 to 3,065,299 _2726 Pf1N1B4_2727 - Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase), at 3,065,330 to 3,065,749 _2727 Pf1N1B4_2728 - ATP-dependent helicase HrpB, at 3,065,847 to 3,068,363 _2728 Pf1N1B4_2729 - endonuclease/exonuclease/phosphatase family protein, at 3,068,505 to 3,069,587 _2729 Pf1N1B4_2730 - FIG00958173: hypothetical protein, at 3,069,676 to 3,070,350 _2730
Group Condition Pf1N1B4_2726 Pf1N1B4_2727 Pf1N1B4_2728 Pf1N1B4_2729 Pf1N1B4_2730
carbon source carbon source Sucrose 0.25mM -0.9 -0.5 -0.0 -0.5 -0.2
nitrogen source nitrogen source nitrite 0.1mM -0.8 -0.5 -0.0 -0.3 -0.3
denitrifying Nitrate reduction, replicate A -0.3 -0.9 +0.1 -0.0 -0.6
carbon source carbon source Sucrose 1mM +0.0 -1.7 -0.1 +0.3 -0.2
temperature LB 37C +0.3 -0.9 -0.6 -0.4 +0.0
stress methylglyoxal 0.008 vol% -0.8 +0.0 +0.2 -0.2 -0.4
stress Choline chloride 200 mM +0.1 -0.3 -0.6 -0.0 -0.3
denitrifying Nitrate reduction, replicate C t1 -0.4 -0.3 +0.3 -0.3 -0.2
stress Paraquat dichloride 0.016 mg/mL -0.4 -0.6 +0.5 +0.0 -0.1
stress copper (II) chloride 2.5 mM +0.3 -0.6 -0.3 -0.0 +0.1
carbon source Fumarate (C) -0.6 -0.1 +0.4 +0.1 -0.4
stress sodium fluoride 12.5 mM -0.5 -0.5 +0.5 +0.1 -0.0
anaerobic Nitrate reduction -0.4 -0.3 +0.4 +0.5 -0.5
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A +0.1 -0.6 +0.3 -0.2 +0.1
nitrogen source Carnitine Hydrochloride (N) +0.3 -0.3 -0.4 -0.1 +0.4
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.0 -0.7 -0.3 +0.4 +0.6
carbon source carbon source Sucrose 0.5mM +0.9 -1.0 +0.3 +0.1 -0.4
anaerobic Nitrate reduction -0.3 -0.4 +0.7 +0.0 -0.0
pH Minimal media pH9 +0.5 -0.6 -0.2 +0.2 +0.1
stress Spectinomycin 0.025 mg/ml -0.5 +0.6 -0.2 +0.4 -0.3
carbon source carbon source Sucrose 5mM -1.0 +0.7 +0.3 +0.2 -0.1
nitrogen source nitrogen source nitrite 0.1mM -0.3 +0.5 +0.2 -0.3 +0.1
denitrifying Nitrate reduction, replicate B t1 +0.4 -0.4 +0.2 +0.3 -0.1
stress Chlorite 1 mM +0.5 +0.3 -0.3 +0.3 -0.1
pH Minimal media pH6 +0.6 -0.4 +0.0 +0.2 +0.1
stress Rifampicin 0.004 mg/ml +0.2 +0.5 -0.2 +0.3 -0.1
anaerobic Nitrate reduction +0.9 +0.1 +0.3 +0.2 -0.2
anaerobic N2E2wt with N1B4m denitrifying repA +0.0 +0.7 +0.3 +0.4 -0.1
carbon source Carnitine Hydrochloride (C) +0.2 +0.2 +0.0 -0.1 +1.1
stress Doxycycline hyclate 0.0001 mg/ml +0.5 +0.6 +0.2 +0.7 +0.0
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