Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_2718 and Pf1N1B4_2719 are separated by 496 nucleotidesPf1N1B4_2719 and Pf1N1B4_2720 are separated by 75 nucleotidesPf1N1B4_2720 and Pf1N1B4_2721 are separated by 185 nucleotidesPf1N1B4_2721 and Pf1N1B4_2722 are separated by 71 nucleotides Pf1N1B4_2718 - Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7), at 3,054,374 to 3,055,102 _2718 Pf1N1B4_2719 - Sensor histidine kinase, at 3,055,599 to 3,057,983 _2719 Pf1N1B4_2720 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 3,058,059 to 3,059,708 _2720 Pf1N1B4_2721 - AMP nucleosidase (EC 3.2.2.4), at 3,059,894 to 3,061,357 _2721 Pf1N1B4_2722 - Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70), at 3,061,429 to 3,062,064 _2722
Group Condition Pf1N1B4_2718 Pf1N1B4_2719 Pf1N1B4_2720 Pf1N1B4_2721 Pf1N1B4_2722
carbon source carbon source Sucrose 0.25mM -0.6 -1.3 -0.8 -1.1 -0.3
stress D-Cycloserine 0.1 mg/ml -0.8 +0.0 -0.1 -2.1 -0.3
pH Minimal media pH9 -2.9 -1.0 +0.1 +0.4 +0.1
pH Minimal media pH9 -2.3 -1.1 +0.1 +0.2 -0.1
stress methylglyoxal 0.008 vol% -0.1 +0.1 -0.3 -2.7 +0.1
nitrogen source nitrogen source nitrite 0.1mM +0.2 -0.2 -0.2 -1.7 -0.5
stress Fusidic 0.125 mg/ml -0.8 +0.9 -0.0 -1.9 -0.3
stress methylglyoxal 0.004 vol% +0.0 -0.1 -0.0 -2.0 +0.1
carbon source carbon source Sucrose 0.5mM +0.5 -1.1 -0.4 -0.7 +0.2
stress Fusidic 0.0625 mg/ml -1.5 +1.3 +0.3 -1.1 -0.2
denitrifying Nitrate reduction, replicate B t2 -1.0 +0.0 -0.0 +0.2 -0.5
stress Cephalothin 2.5 mg/ml -0.6 +0.3 +0.2 -0.8 -0.1
carbon source carbon source Sucrose 1mM +0.5 -0.9 -0.1 -0.5 +0.0
denitrifying Nitrate reduction, replicate B t1 -1.2 +0.1 +0.1 +0.1 -0.1
stress Doxycycline hyclate 0.0001 mg/ml -0.9 +0.5 +0.0 -0.3 +0.1
carbon source carbon source Sucrose 0.25mM +0.8 -1.5 +0.3 +0.1 -0.3
nitrogen source nitrogen source nitrite 1mM +0.4 -0.0 +0.3 -1.1 +0.0
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.7 +0.2 -0.4 +0.2 +0.2
stress Paraquat dichloride 0.016 mg/mL +0.8 +0.1 -0.2 -1.0 +0.1
stress nitrite 10 mM -0.2 +0.2 +0.2 -0.8 +0.4
carbon source carbon source Sucrose 1mM +0.9 -0.7 +0.2 -0.4 -0.1
stress Nalidixic 0.004 mg/ml -0.7 +0.6 -0.0 -0.3 +0.4
carbon source Putrescine (C) -0.7 +0.5 +0.2 -0.1 +0.0
stress Choline chloride 200 mM +0.5 -0.5 +0.1 -0.1 +0.8
stress nitrite 10 mM +0.0 +0.5 +0.3 +0.3 -0.4
carbon source octanoate (C) +0.3 +0.5 +0.3 -0.4 +0.3
carbon source D-Fructose (C) +0.2 +0.3 +0.1 +0.7 -0.2
stress Benzethonium chloride 0.0125 mM -0.3 +1.3 +0.3 -0.6 +0.5
carbon source L-Arginine (C) +0.2 +0.9 +0.1 -0.1 +0.2
carbon source D-Fructose (C) +0.0 +0.5 -0.1 +0.9 +0.1
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