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Protein
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Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_2711 and Pf1N1B4_2712 are separated by 214 nucleotides
Pf1N1B4_2712 and Pf1N1B4_2713 are separated by 86 nucleotides
Pf1N1B4_2713 and Pf1N1B4_2714 are separated by 139 nucleotides
Pf1N1B4_2714 and Pf1N1B4_2715 are separated by 27 nucleotides
Pf1N1B4_2711 - Phosphate starvation-inducible protein PhoH, predicted ATPase, at 3,048,488 to 3,049,504
_2711
Pf1N1B4_2712 - tRNA-i(6)A37 methylthiotransferase, at 3,049,719 to 3,051,095
_2712
Pf1N1B4_2713 - FIG00955339: hypothetical protein, at 3,051,182 to 3,051,511
_2713
Pf1N1B4_2714 - FOG: TPR repeat, SEL1 subfamily, at 3,051,651 to 3,052,205
_2714
Pf1N1B4_2715 - hypothetical protein, at 3,052,233 to 3,052,397
_2715
Group
Condition
Pf1N1B4
_2711
Pf1N1B4
_2712
Pf1N1B4
_2713
Pf1N1B4
_2714
Pf1N1B4
_2715
nitrogen source
nitrogen source nitrite 0.1mM
-0.8
-0.1
-1.7
+0.2
-0.1
carbon source
carbon source Sucrose 1mM
-1.6
-0.7
-1.2
+0.7
+0.8
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
+0.7
-0.5
-1.2
-0.6
-0.2
stress
Cobalt chloride 0.3125 mM
-2.0
-0.0
+0.1
+0.0
+0.2
stress
Carbenicillin 2 mg/ml
-0.4
+0.2
+1.0
-1.4
-1.1
stress
Cisplatin 0.006 mg/ml
+0.0
-0.9
+0.8
-0.5
-1.0
nitrogen source
nitrogen source nitrate 20mM
-0.3
-1.0
-0.7
+0.1
+0.5
pH
Minimal media pH7
-0.7
-0.7
-0.2
-0.3
+0.5
nitrogen source
Cytidine (N)
-1.3
-0.6
+0.4
-0.3
+0.5
carbon source
octanoate (C)
+0.7
-0.9
+0.2
-0.4
-0.9
pH
Minimal media pH9
-1.3
-1.2
-1.3
+1.4
+1.2
pH
Minimal media pH9
-1.0
-1.3
-1.1
+1.0
+1.3
carbon source
carbon source Sucrose 0.25mM
-0.7
-1.1
-1.0
+0.7
+1.0
temperature
LB 20C
-0.2
-0.6
-0.9
+0.3
+0.5
nitrogen source
nitrogen source nitrite 0.5mM
+0.5
-0.6
-1.2
+0.3
+0.1
survival
4C survival; 24 hrs
-0.2
-0.5
-0.9
+0.5
+0.3
carbon source
carbon source Sucrose 0.5mM
-0.8
-0.4
-1.3
+0.9
+1.2
stress
Gentamicin 0.001 mg/ml
+0.4
-1.2
+0.7
+0.0
-0.0
stress
methylglyoxal 0.004 vol%
-0.9
+0.3
+0.5
-0.2
+0.2
stress
Cisplatin 0.006 mg/ml
+0.3
-1.1
+1.2
+0.3
-0.7
anaerobic
N2E2wt with N1B4m denitrifying repB
+0.8
-0.0
+0.5
-0.6
-0.3
carbon source
D-Galactose (C)
-0.1
-0.9
+0.5
+0.6
+0.2
stress
Sisomicin 0.001 mg/ml
-0.2
-0.5
+1.1
+0.2
-0.2
carbon source
carbon source Sucrose 5mM
+0.8
-0.5
-0.5
+0.5
+0.2
carbon source
carbon source Sucrose 1mM
-1.5
-0.2
-0.5
+1.5
+1.2
carbon source
D-Fructose (C)
+0.5
-0.4
+1.0
-0.1
-0.5
stress
Nalidixic 0.004 mg/ml
-1.0
+0.7
+1.1
+0.2
-0.2
stress
Choline Lysine 6 mM
+0.3
-0.2
-0.9
+0.9
+0.8
carbon source
carbon source Sucrose 0.25mM
+0.4
-0.2
-0.3
+0.7
+1.1
stress
Doxycycline hyclate 0.0001 mg/ml
+0.5
+0.7
+0.9
-0.0
+0.1
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