Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_2634 and Pf1N1B4_2635 are separated by 69 nucleotidesPf1N1B4_2635 and Pf1N1B4_2636 are separated by 175 nucleotidesPf1N1B4_2636 and Pf1N1B4_2637 are separated by 32 nucleotidesPf1N1B4_2637 and Pf1N1B4_2638 overlap by 8 nucleotides Pf1N1B4_2634 - Lhr-like helicases, at 2,972,638 to 2,976,840 _2634 Pf1N1B4_2635 - hypothetical protein, at 2,976,910 to 2,977,119 _2635 Pf1N1B4_2636 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 2,977,295 to 2,977,411 _2636 Pf1N1B4_2637 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 2,977,444 to 2,978,163 _2637 Pf1N1B4_2638 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 2,978,156 to 2,979,085 _2638
Group Condition Pf1N1B4_2634 Pf1N1B4_2635 Pf1N1B4_2636 Pf1N1B4_2637 Pf1N1B4_2638
carbon source carbon source Sucrose 5mM +0.2 -1.9 N.D. -0.1 N.D.
stress nitrite 10 mM -0.1 -0.5 N.D. -0.9 N.D.
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 +0.4 -1.2 N.D. +0.6 N.D.
carbon source carbon source Sucrose 0.5mM -0.0 -0.6 N.D. +1.2 N.D.
denitrifying Nitrate reduction, replicate C +0.3 -0.5 N.D. +0.9 N.D.
carbon source carbon source Sucrose 1mM +0.0 -0.4 N.D. +1.0 N.D.
stress methylglyoxal 0.008 vol% -0.3 +0.6 N.D. +0.4 N.D.
stress 1-ethyl-3-methylimidazolium chloride 80 mM -0.2 +0.6 N.D. +0.6 N.D.
stress Fusidic 0.0625 mg/ml -0.1 +0.9 N.D. +0.4 N.D.
denitrifying Nitrate reduction, replicate A t2 +0.0 +0.6 N.D. +0.7 N.D.
stress 1-ethyl-3-methylimidazolium lysine 5 mM -0.2 +0.5 N.D. +1.1 N.D.
carbon source Carnitine Hydrochloride (C) -0.1 +0.5 N.D. +0.9 N.D.
denitrifying Nitrate reduction, replicate A t1 +0.1 +0.6 N.D. +0.6 N.D.
stress Choline Lysine 6 mM +0.0 -0.2 N.D. +1.5 N.D.
stress Chloride 300 mM +0.1 +0.8 N.D. +0.5 N.D.
carbon source L-Arginine (C) +0.0 +0.6 N.D. +0.8 N.D.
carbon source L-Citrulline (C) -0.0 +0.8 N.D. +0.7 N.D.
carbon source carbon source Sucrose 0.25mM -0.2 +0.9 N.D. +0.8 N.D.
lb LB +0.1 +1.3 N.D. +0.1 N.D.
carbon source L-Leucine (C) -0.0 +0.3 N.D. +1.3 N.D.
lb LB -0.0 +1.0 N.D. +0.6 N.D.
carbon source carbon source Sucrose 1mM -0.0 +0.6 N.D. +1.0 N.D.
pH Minimal media pH9 -0.0 -0.1 N.D. +1.9 N.D.
stress Choline chloride 200 mM +0.0 +1.2 N.D. +0.7 N.D.
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.0 +1.0 N.D. +0.9 N.D.
stress Gentamicin 0.001 mg/ml -0.1 +1.2 N.D. +0.9 N.D.
stress Fusidic 0.125 mg/ml -0.1 +1.0 N.D. +1.1 N.D.
stress Sisomicin 0.001 mg/ml +0.3 +1.0 N.D. +1.0 N.D.
stress Carbenicillin 2 mg/ml +0.1 +1.9 N.D. +0.7 N.D.
pH Minimal media pH9 +0.2 +0.8 N.D. +1.9 N.D.
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