Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_2626 and Pf1N1B4_2627 are separated by 12 nucleotidesPf1N1B4_2627 and Pf1N1B4_2628 overlap by 17 nucleotidesPf1N1B4_2628 and Pf1N1B4_2629 are separated by 17 nucleotidesPf1N1B4_2629 and Pf1N1B4_2630 overlap by 4 nucleotides Pf1N1B4_2626 - Biotin carboxylase (EC 6.3.4.14), at 2,964,796 to 2,965,881 _2626 Pf1N1B4_2627 - Prolyl oligopeptidase family protein, at 2,965,894 to 2,966,652 _2627 Pf1N1B4_2628 - hypothetical protein, at 2,966,636 to 2,966,764 _2628 Pf1N1B4_2629 - Inner membrane protein YbhQ, at 2,966,782 to 2,967,768 _2629 Pf1N1B4_2630 - Cardiolipin synthetase (EC 2.7.8.-), at 2,967,765 to 2,969,051 _2630
Group Condition Pf1N1B4_2626 Pf1N1B4_2627 Pf1N1B4_2628 Pf1N1B4_2629 Pf1N1B4_2630
stress Gentamicin 0.001 mg/ml -0.1 -0.7 N.D. -0.1 -0.3
carbon source a-Ketoglutaric (C) -0.4 -0.4 N.D. +0.0 -0.1
carbon source D,L-Lactate (C) -0.0 -0.3 N.D. +0.1 -0.4
lb LB +0.1 -0.4 N.D. -0.3 -0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A -0.3 -0.3 N.D. -0.1 +0.1
lb LB +0.0 -0.2 N.D. +0.2 -0.4
carbon source pyruvate (C) +0.2 -0.2 N.D. -0.2 -0.2
stress Fusidic 0.125 mg/ml -0.2 -0.2 N.D. +0.2 -0.2
stress Doxycycline hyclate 0.0001 mg/ml +0.2 -0.2 N.D. -0.2 -0.2
carbon source carbon source Sucrose 0.5mM +0.2 -0.1 N.D. -0.6 +0.2
stress Choline Lysine 6 mM +0.5 -0.4 N.D. -0.1 -0.2
stress Carbenicillin 2 mg/ml -0.2 -0.2 N.D. +0.3 -0.1
carbon source carbon source Sucrose 0.25mM +0.0 -0.5 N.D. +0.3 +0.1
stress Bacitracin 2 mg/ml +0.2 -0.6 N.D. +0.2 +0.0
carbon source carbon source Sucrose 5mM +0.2 +0.2 N.D. -0.7 +0.2
carbon source D-Glucose (C) +0.1 -0.2 N.D. +0.3 -0.2
stress Chloride 300 mM -0.0 +0.3 N.D. +0.1 -0.5
carbon source carbon source Sucrose 1mM +0.1 +0.3 N.D. -0.6 +0.2
nitrogen source Putrescine (N) -0.1 +0.2 N.D. +0.2 -0.3
lb LB +0.2 +0.0 N.D. +0.3 -0.3
nitrogen source Ammonium chloride (N) -0.1 +0.5 N.D. -0.0 -0.2
pH Minimal media pH9 -0.3 +0.3 N.D. -0.0 +0.2
stress Cobalt chloride 0.3125 mM +0.1 -0.3 N.D. +0.2 +0.3
nitrogen source D-Glucosamine Hydrochloride (N) +0.2 +0.3 N.D. +0.2 -0.3
temperature LB 10C +0.3 -0.3 N.D. +0.3 +0.2
carbon source Tween 20 (C) +0.3 +0.2 N.D. +0.1 -0.2
denitrifying Nitrate reduction, replicate A t2 +0.3 +0.2 N.D. +0.3 -0.1
anaerobic Nitrate reduction -0.2 +0.7 N.D. +0.1 +0.0
anaerobic Nitrate reduction +0.1 +0.7 N.D. +0.2 +0.1
carbon source carbon source Sucrose 1mM +0.4 +0.3 N.D. +0.4 +0.3
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