Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_2555 and Pf1N1B4_2556 are separated by 50 nucleotidesPf1N1B4_2556 and Pf1N1B4_2557 are separated by 70 nucleotidesPf1N1B4_2557 and Pf1N1B4_2558 are separated by 33 nucleotidesPf1N1B4_2558 and Pf1N1B4_2559 are separated by 135 nucleotides Pf1N1B4_2555 - Oxidoreductase, short chain dehydrogenase/reductase family, at 2,897,423 to 2,898,223 _2555 Pf1N1B4_2556 - FIG00954311: hypothetical protein, at 2,898,274 to 2,899,251 _2556 Pf1N1B4_2557 - 2-nitropropane dioxygenase (EC 1.13.11.32), at 2,899,322 to 2,900,290 _2557 Pf1N1B4_2558 - Protoporphyrinogen IX oxidase, novel form, HemJ (EC 1.3.-.-), at 2,900,324 to 2,900,755 _2558 Pf1N1B4_2559 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 2,900,891 to 2,901,925 _2559
Group Condition Pf1N1B4_2555 Pf1N1B4_2556 Pf1N1B4_2557 Pf1N1B4_2558 Pf1N1B4_2559
pH Minimal media pH9 -0.5 -0.6 -1.0 N.D. N.D.
stress Carbenicillin 2 mg/ml -0.7 -0.3 -0.3 N.D. N.D.
nitrogen source D-Glucosamine Hydrochloride (N) -0.6 -0.1 -0.5 N.D. N.D.
carbon source carbon source Sucrose 5mM -0.5 -0.3 -0.3 N.D. N.D.
nitrogen source L-Methionine (N) -0.6 -0.1 -0.3 N.D. N.D.
stress nitrite 10 mM -0.6 -0.3 -0.1 N.D. N.D.
carbon source Fumarate (C) -0.3 -0.3 -0.4 N.D. N.D.
stress Spectinomycin 0.05 mg/ml -0.4 -0.1 -0.4 N.D. N.D.
carbon source carbon source Sucrose 1mM -0.2 -0.2 -0.5 N.D. N.D.
denitrifying Nitrate reduction, replicate C t3 -0.2 -0.2 -0.4 N.D. N.D.
stress benzoic 0.58 mg/ml -0.2 -0.3 -0.2 N.D. N.D.
anaerobic N2E2wt with N1B4m denitrifying repA -0.3 +0.0 -0.5 N.D. N.D.
carbon source Fumarate (C) -0.3 -0.1 -0.3 N.D. N.D.
stress Nalidixic 0.004 mg/ml -0.2 +0.0 -0.5 N.D. N.D.
denitrifying Nitrate reduction, replicate B -0.3 +0.1 -0.4 N.D. N.D.
carbon source octanoate (C) -0.7 -0.0 +0.2 N.D. N.D.
denitrifying Nitrate reduction, replicate C -0.5 +0.2 -0.2 N.D. N.D.
stress Fusidic 0.125 mg/ml +0.3 -0.2 -0.5 N.D. N.D.
carbon source carbon source Sucrose 0.25mM +0.4 +0.1 -0.9 N.D. N.D.
pH Minimal media pH9 -0.1 +0.2 -0.4 N.D. N.D.
stress Doxycycline hyclate 0.0001 mg/ml -0.6 -0.1 +0.4 N.D. N.D.
anaerobic Nitrate reduction -0.1 +0.3 -0.5 N.D. N.D.
carbon source carbon source Sucrose 5mM -0.3 +0.5 -0.5 N.D. N.D.
carbon source carbon source Sucrose 0.25mM +0.3 -0.3 -0.2 N.D. N.D.
stress Benzethonium chloride 0.0125 mM -0.4 +0.2 +0.2 N.D. N.D.
anaerobic Nitrate reduction -0.4 +0.1 +0.3 N.D. N.D.
nitrogen source nitrogen source nitrite 0.1mM -0.1 -0.3 +0.4 N.D. N.D.
stress Aluminum chloride 2.5 mM -0.3 -0.1 +0.6 N.D. N.D.
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.2 +0.5 +0.1 N.D. N.D.
temperature LB 37C +0.3 +0.5 -0.3 N.D. N.D.
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