Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_5192 and Pf1N1B4_2512 are separated by 15 nucleotidesPf1N1B4_2512 and Pf1N1B4_2513 are separated by 122 nucleotidesPf1N1B4_2513 and Pf1N1B4_2514 are separated by 67 nucleotidesPf1N1B4_2514 and Pf1N1B4_2515 are separated by 481 nucleotides Pf1N1B4_5192 - tRNA-Met-CAT, at 2,846,952 to 2,847,025 _5192 Pf1N1B4_2512 - sensory box/GGDEF domain/EAL domain protein, at 2,847,041 to 2,850,787 _2512 Pf1N1B4_2513 - RNA polymerase sigma factor RpoD, at 2,850,910 to 2,852,757 _2513 Pf1N1B4_2514 - DNA primase (EC 2.7.7.-), at 2,852,825 to 2,854,798 _2514 Pf1N1B4_2515 - SSU ribosomal protein S21p, at 2,855,280 to 2,855,495 _2515
Group Condition Pf1N1B4_5192 Pf1N1B4_2512 Pf1N1B4_2513 Pf1N1B4_2514 Pf1N1B4_2515
carbon source carbon source Sucrose 1mM N.D. -0.5 N.D. N.D. N.D.
anaerobic Nitrate reduction N.D. -0.4 N.D. N.D. N.D.
temperature LB 37C N.D. -0.3 N.D. N.D. N.D.
stress Doxycycline hyclate 0.0001 mg/ml N.D. -0.3 N.D. N.D. N.D.
anaerobic Nitrate reduction N.D. -0.3 N.D. N.D. N.D.
stress Chloride 300 mM N.D. -0.2 N.D. N.D. N.D.
stress Bacitracin 2 mg/ml N.D. -0.2 N.D. N.D. N.D.
stress Nalidixic 0.004 mg/ml N.D. -0.2 N.D. N.D. N.D.
carbon source D-Glucose (C) N.D. -0.2 N.D. N.D. N.D.
nitrogen source L-Leucine (N) N.D. -0.2 N.D. N.D. N.D.
stress Cholin acetate 10 mM N.D. -0.2 N.D. N.D. N.D.
carbon source carbon source Sucrose 0.25mM N.D. -0.2 N.D. N.D. N.D.
anaerobic N2E2wt with N1B4m denitrifying repB N.D. -0.2 N.D. N.D. N.D.
carbon source D-Lactate (C) N.D. +0.2 N.D. N.D. N.D.
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 N.D. +0.2 N.D. N.D. N.D.
denitrifying Nitrate reduction, replicate B t2 N.D. +0.2 N.D. N.D. N.D.
carbon source L-Arabinose (C) N.D. +0.2 N.D. N.D. N.D.
denitrifying Nitrate reduction, replicate B t1 N.D. +0.2 N.D. N.D. N.D.
carbon source D-Ribose (C) N.D. +0.2 N.D. N.D. N.D.
denitrifying Nitrate reduction, replicate C t1 N.D. +0.3 N.D. N.D. N.D.
temperature LB 37C N.D. +0.3 N.D. N.D. N.D.
carbon source carbon source Sucrose 5mM N.D. +0.3 N.D. N.D. N.D.
carbon source carbon source Sucrose 0.25mM N.D. +0.3 N.D. N.D. N.D.
denitrifying Nitrate reduction, replicate C t2 N.D. +0.4 N.D. N.D. N.D.
nitrogen source nitrogen source Ammonium chloride 20mM N.D. +0.4 N.D. N.D. N.D.
denitrifying Nitrate reduction, replicate A N.D. +0.4 N.D. N.D. N.D.
denitrifying Nitrate reduction, replicate A t2 N.D. +0.4 N.D. N.D. N.D.
carbon source L-Isoleucine (C) N.D. +0.4 N.D. N.D. N.D.
denitrifying Nitrate reduction, replicate C t3 N.D. +0.5 N.D. N.D. N.D.
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 N.D. +0.5 N.D. N.D. N.D.
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