Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_2480 and Pf1N1B4_2481 are separated by 162 nucleotidesPf1N1B4_2481 and Pf1N1B4_2482 are separated by 194 nucleotidesPf1N1B4_2482 and Pf1N1B4_2483 are separated by 130 nucleotidesPf1N1B4_2483 and Pf1N1B4_2484 are separated by 24 nucleotides Pf1N1B4_2480 - Protein rarD, at 2,813,907 to 2,814,794 _2480 Pf1N1B4_2481 - YihE protein, required for LPS synthesis, at 2,814,957 to 2,815,931 _2481 Pf1N1B4_2482 - Putative phosphatase, at 2,816,126 to 2,818,027 _2482 Pf1N1B4_2483 - DNA-binding domain of ModE / Molybdate-binding domain of ModE, at 2,818,158 to 2,818,922 _2483 Pf1N1B4_2484 - hypothetical protein, at 2,818,947 to 2,819,150 _2484
Group Condition Pf1N1B4_2480 Pf1N1B4_2481 Pf1N1B4_2482 Pf1N1B4_2483 Pf1N1B4_2484
temperature LB 37C +0.3 -0.2 +0.1 -1.3 -1.7
temperature LB 20C -0.2 -0.3 -0.1 -0.2 -1.2
stress Fusidic 0.125 mg/ml +0.2 -0.0 -0.3 -0.6 -0.9
stress Cobalt chloride 0.3125 mM -0.0 -0.6 -0.0 +0.2 -1.2
temperature LB 37C -0.1 -0.3 -0.2 +0.1 -1.1
carbon source carbon source Sucrose 1mM -0.5 +0.0 +0.6 -2.1 +0.5
stress Rifampicin 0.004 mg/ml +0.1 -0.6 -0.1 +0.2 -1.0
stress Paraquat dichloride 0.016 mg/mL -0.2 -0.2 +0.0 -1.2 +0.3
carbon source carbon source Sucrose 0.25mM +0.1 -1.2 +0.3 -0.1 -0.0
pH Minimal media pH9 +0.1 -0.7 +0.2 -0.1 -0.4
stress 1-ethyl-3-methylimidazolium lysine 5 mM +0.0 -0.5 -0.0 +0.7 -0.7
carbon source carbon source Sucrose 0.25mM -0.2 +0.2 +0.6 -1.2 +0.1
stress methylglyoxal 0.008 vol% -0.3 -0.2 -0.3 +0.7 -0.4
stress Sisomicin 0.001 mg/ml -0.3 -0.0 -0.2 +0.8 -0.6
stress Doxycycline hyclate 0.0001 mg/ml -0.0 -0.6 +0.3 +0.5 -0.5
carbon source L-Histidine (C) +0.2 +0.0 -0.0 -1.0 +0.5
stress Chloride 300 mM -0.0 +0.1 -0.1 +0.6 -0.9
denitrifying Nitrate reduction, replicate C t1 +0.0 -0.3 -0.1 +0.7 -0.5
anaerobic N2E2wt with N1B4m denitrifying repB +0.1 -0.3 -0.1 +0.8 -0.7
carbon source octanoate (C) +0.7 -0.3 +0.2 -0.7 -0.0
carbon source Putrescine (C) +0.4 -0.1 +0.3 -0.7 -0.1
stress Tetracycline 0.0004 mg/ml -0.0 -0.4 +0.2 -0.4 +0.6
anaerobic Nitrate reduction -0.3 +0.5 -0.1 +0.6 -0.5
denitrifying Nitrate reduction, replicate C t3 -0.1 -0.2 -0.0 +1.0 -0.4
nitrogen source L-Serine (N) -0.6 +0.3 -0.1 +0.2 +0.4
anaerobic Nitrate reduction -0.1 +0.4 +0.1 +1.1 -0.8
carbon source D-Galactose (C) +0.2 -0.4 +0.2 +0.4 +0.5
carbon source L-Isoleucine (C) +0.4 +0.2 +0.1 -0.5 +0.8
nitrogen source nitrogen source nitrite 0.1mM +0.3 +0.1 -0.0 -0.3 +0.9
carbon source carbon source Sucrose 1mM +0.0 +0.5 +0.5 +0.2 +0.4
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