Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_2479 and Pf1N1B4_2480 are separated by 214 nucleotidesPf1N1B4_2480 and Pf1N1B4_2481 are separated by 162 nucleotidesPf1N1B4_2481 and Pf1N1B4_2482 are separated by 194 nucleotidesPf1N1B4_2482 and Pf1N1B4_2483 are separated by 130 nucleotides Pf1N1B4_2479 - Glycine cleavage system transcriptional antiactivator GcvR, at 2,813,174 to 2,813,692 _2479 Pf1N1B4_2480 - Protein rarD, at 2,813,907 to 2,814,794 _2480 Pf1N1B4_2481 - YihE protein, required for LPS synthesis, at 2,814,957 to 2,815,931 _2481 Pf1N1B4_2482 - Putative phosphatase, at 2,816,126 to 2,818,027 _2482 Pf1N1B4_2483 - DNA-binding domain of ModE / Molybdate-binding domain of ModE, at 2,818,158 to 2,818,922 _2483
Group Condition Pf1N1B4_2479 Pf1N1B4_2480 Pf1N1B4_2481 Pf1N1B4_2482 Pf1N1B4_2483
temperature LB 37C -2.1 +0.3 -0.2 +0.1 -1.3
carbon source carbon source Sucrose 0.25mM -0.9 +0.1 -1.2 +0.3 -0.1
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.4 -0.1 -0.0 -0.3 -0.8
stress methylglyoxal 0.008 vol% -1.4 -0.3 -0.2 -0.3 +0.7
carbon source carbon source Sucrose 0.25mM -0.8 -0.2 +0.2 +0.6 -1.2
stress Fusidic 0.0625 mg/ml -1.5 +0.2 +0.1 +0.4 -0.6
stress Carbenicillin 2 mg/ml -1.4 +0.1 -0.3 -0.2 +0.4
carbon source carbon source Sucrose 20mM -0.9 -0.3 -0.3 +0.3 -0.2
stress Cobalt chloride 0.3125 mM -0.8 -0.0 -0.6 -0.0 +0.2
carbon source D-Glucose (C) -0.4 +0.1 -0.3 +0.1 -0.7
stress Nalidixic 0.004 mg/ml -0.9 -0.1 -0.3 -0.1 +0.2
carbon source m-Inositol (C) -0.9 +0.1 +0.3 +0.1 -0.7
carbon source carbon source Sucrose 1mM +0.8 -0.5 +0.0 +0.6 -2.1
stress Rifampicin 0.004 mg/ml -0.7 +0.1 -0.6 -0.1 +0.2
stress Paraquat dichloride 0.016 mg/mL +0.4 -0.2 -0.2 +0.0 -1.2
nitrogen source L-Serine (N) -0.8 -0.6 +0.3 -0.1 +0.2
carbon source D-Mannose (C) -0.8 +0.3 +0.3 +0.1 -0.5
stress 1-ethyl-3-methylimidazolium lysine 5 mM -0.6 +0.0 -0.5 -0.0 +0.7
carbon source carbon source Sucrose 0.5mM -1.0 -0.0 +0.4 +0.2 +0.1
carbon source octanoate (C) +0.1 +0.7 -0.3 +0.2 -0.7
pH Minimal media pH9 -0.5 +0.2 -0.2 +0.1 +0.5
stress Sisomicin 0.001 mg/ml +0.4 -0.3 -0.0 -0.2 +0.8
nitrogen source Uridine (N) +1.0 -0.1 -0.2 +0.2 -0.3
anaerobic Nitrate reduction +0.3 -0.3 +0.5 -0.1 +0.6
denitrifying Nitrate reduction, replicate C t1 +0.7 +0.0 -0.3 -0.1 +0.7
stress Doxycycline hyclate 0.0001 mg/ml +0.8 -0.0 -0.6 +0.3 +0.5
nitrogen source Thymine (N) +1.1 -0.3 +0.2 -0.1 +0.1
nitrogen source nitrogen source nitrite 0.1mM +1.1 +0.3 +0.1 -0.0 -0.3
nitrogen source Ammonium chloride (N) +0.4 -0.0 -0.1 +0.2 +0.9
anaerobic Nitrate reduction +0.2 -0.1 +0.4 +0.1 +1.1
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