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Cofit
Protein
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Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_2479 and Pf1N1B4_2480 are separated by 214 nucleotides
Pf1N1B4_2480 and Pf1N1B4_2481 are separated by 162 nucleotides
Pf1N1B4_2481 and Pf1N1B4_2482 are separated by 194 nucleotides
Pf1N1B4_2482 and Pf1N1B4_2483 are separated by 130 nucleotides
Pf1N1B4_2479 - Glycine cleavage system transcriptional antiactivator GcvR, at 2,813,174 to 2,813,692
_2479
Pf1N1B4_2480 - Protein rarD, at 2,813,907 to 2,814,794
_2480
Pf1N1B4_2481 - YihE protein, required for LPS synthesis, at 2,814,957 to 2,815,931
_2481
Pf1N1B4_2482 - Putative phosphatase, at 2,816,126 to 2,818,027
_2482
Pf1N1B4_2483 - DNA-binding domain of ModE / Molybdate-binding domain of ModE, at 2,818,158 to 2,818,922
_2483
Group
Condition
Pf1N1B4
_2479
Pf1N1B4
_2480
Pf1N1B4
_2481
Pf1N1B4
_2482
Pf1N1B4
_2483
temperature
LB 37C
-2.1
+0.3
-0.2
+0.1
-1.3
carbon source
carbon source Sucrose 0.25mM
-0.9
+0.1
-1.2
+0.3
-0.1
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
-0.4
-0.1
-0.0
-0.3
-0.8
stress
methylglyoxal 0.008 vol%
-1.4
-0.3
-0.2
-0.3
+0.7
carbon source
carbon source Sucrose 0.25mM
-0.8
-0.2
+0.2
+0.6
-1.2
stress
Fusidic 0.0625 mg/ml
-1.5
+0.2
+0.1
+0.4
-0.6
stress
Carbenicillin 2 mg/ml
-1.4
+0.1
-0.3
-0.2
+0.4
carbon source
carbon source Sucrose 20mM
-0.9
-0.3
-0.3
+0.3
-0.2
stress
Cobalt chloride 0.3125 mM
-0.8
-0.0
-0.6
-0.0
+0.2
carbon source
D-Glucose (C)
-0.4
+0.1
-0.3
+0.1
-0.7
stress
Nalidixic 0.004 mg/ml
-0.9
-0.1
-0.3
-0.1
+0.2
carbon source
m-Inositol (C)
-0.9
+0.1
+0.3
+0.1
-0.7
carbon source
carbon source Sucrose 1mM
+0.8
-0.5
+0.0
+0.6
-2.1
stress
Rifampicin 0.004 mg/ml
-0.7
+0.1
-0.6
-0.1
+0.2
stress
Paraquat dichloride 0.016 mg/mL
+0.4
-0.2
-0.2
+0.0
-1.2
nitrogen source
L-Serine (N)
-0.8
-0.6
+0.3
-0.1
+0.2
carbon source
D-Mannose (C)
-0.8
+0.3
+0.3
+0.1
-0.5
stress
1-ethyl-3-methylimidazolium lysine 5 mM
-0.6
+0.0
-0.5
-0.0
+0.7
carbon source
carbon source Sucrose 0.5mM
-1.0
-0.0
+0.4
+0.2
+0.1
carbon source
octanoate (C)
+0.1
+0.7
-0.3
+0.2
-0.7
pH
Minimal media pH9
-0.5
+0.2
-0.2
+0.1
+0.5
stress
Sisomicin 0.001 mg/ml
+0.4
-0.3
-0.0
-0.2
+0.8
nitrogen source
Uridine (N)
+1.0
-0.1
-0.2
+0.2
-0.3
anaerobic
Nitrate reduction
+0.3
-0.3
+0.5
-0.1
+0.6
denitrifying
Nitrate reduction, replicate C t1
+0.7
+0.0
-0.3
-0.1
+0.7
stress
Doxycycline hyclate 0.0001 mg/ml
+0.8
-0.0
-0.6
+0.3
+0.5
nitrogen source
Thymine (N)
+1.1
-0.3
+0.2
-0.1
+0.1
nitrogen source
nitrogen source nitrite 0.1mM
+1.1
+0.3
+0.1
-0.0
-0.3
nitrogen source
Ammonium chloride (N)
+0.4
-0.0
-0.1
+0.2
+0.9
anaerobic
Nitrate reduction
+0.2
-0.1
+0.4
+0.1
+1.1
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