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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_2466 and Pf1N1B4_2467 are separated by 1 nucleotides
Pf1N1B4_2467 and Pf1N1B4_2468 are separated by 236 nucleotides
Pf1N1B4_2468 and Pf1N1B4_2469 are separated by 213 nucleotides
Pf1N1B4_2469 and Pf1N1B4_2470 are separated by 157 nucleotides
Pf1N1B4_2466 - Two-component response regulator CreC, at 2,795,973 to 2,797,406
_2466
Pf1N1B4_2467 - Acetyltransferase, at 2,797,408 to 2,798,418
_2467
Pf1N1B4_2468 - Inner membrane protein CreD, at 2,798,655 to 2,800,025
_2468
Pf1N1B4_2469 - Glutathione S-transferase family protein, at 2,800,239 to 2,800,904
_2469
Pf1N1B4_2470 - FIG00953361: hypothetical protein, at 2,801,062 to 2,801,241
_2470
Group
Condition
Pf1N1B4
_2466
Pf1N1B4
_2467
Pf1N1B4
_2468
Pf1N1B4
_2469
Pf1N1B4
_2470
nitrogen source
D-Glucosamine Hydrochloride (N)
-0.2
-0.1
-0.1
-1.1
N.D.
temperature
LB 37C
-0.4
-0.4
-0.2
-0.3
N.D.
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
-0.1
+0.1
-0.3
-0.9
N.D.
nitrogen source
nitrate (N)
-0.1
-0.0
-0.1
-0.9
N.D.
nitrogen source
Carnitine Hydrochloride (N)
+0.2
-0.3
-0.1
-0.9
N.D.
nitrogen source
nitrogen source nitrate 20mM
-0.1
-0.4
+0.3
-0.9
N.D.
nitrogen source
Cytidine (N)
+0.1
-0.1
-0.2
-0.8
N.D.
carbon source
L-Histidine (C)
-0.2
-0.3
-0.3
-0.2
N.D.
stress
Fusidic 0.125 mg/ml
-0.4
+0.1
-0.1
-0.4
N.D.
carbon source
carbon source Sucrose 5mM
+0.0
+0.4
-0.1
-1.2
N.D.
anaerobic
N2E2wt with N1B4m denitrifying repB
+0.0
-0.1
+0.1
-0.9
N.D.
pH
Minimal media pH9
-0.3
+0.1
+0.0
-0.6
N.D.
stress
Bacitracin 2 mg/ml
+0.3
-0.3
-0.1
-0.4
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
+0.1
+0.0
+0.1
-0.8
N.D.
stress
methylglyoxal 0.004 vol%
+0.1
-0.0
+0.1
-0.6
N.D.
carbon source
carbon source Sucrose 5mM
-0.1
+0.3
-0.2
-0.4
N.D.
stress
Carbenicillin 2 mg/ml
+0.3
-0.1
+0.2
-0.5
N.D.
stress
Choline Lysine 6 mM
+0.3
+0.1
-0.5
+0.2
N.D.
stress
Rifampicin 0.004 mg/ml
+0.1
-0.3
-0.1
+0.5
N.D.
carbon source
carbon source Sucrose 0.25mM
+0.1
+0.1
+0.5
-0.4
N.D.
stress
Benzethonium chloride 0.0125 mM
+0.3
-0.1
-0.3
+0.6
N.D.
stress
Spectinomycin 0.025 mg/ml
+0.3
-0.1
-0.1
+0.5
N.D.
carbon source
D-Lactate (C)
-0.1
+0.1
+0.4
+0.3
N.D.
stress
Chloride 300 mM
-0.0
-0.1
+0.2
+0.6
N.D.
anaerobic
N2E2wt with N1B4m denitrifying repA
+0.2
-0.1
+0.1
+0.6
N.D.
carbon source
carbon source Sucrose 1mM
-0.3
+0.1
+0.3
+0.8
N.D.
anaerobic
Nitrate reduction
-0.1
+0.2
+0.0
+0.8
N.D.
carbon source
carbon source Sucrose 1mM
+0.2
+0.5
-0.3
+0.5
N.D.
denitrifying
Nitrate reduction, replicate C
-0.0
+0.3
+0.3
+0.5
N.D.
nitrogen source
nitrogen source Ammonium chloride 20mM
+0.2
+0.1
+0.1
+0.6
N.D.
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