Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_2360 and Pf1N1B4_2361 are separated by 715 nucleotidesPf1N1B4_2361 and Pf1N1B4_2362 overlap by 1 nucleotidesPf1N1B4_2362 and Pf1N1B4_2363 overlap by 1 nucleotidesPf1N1B4_2363 and Pf1N1B4_2364 overlap by 8 nucleotides Pf1N1B4_2360 - hypothetical protein, at 2,677,520 to 2,677,897 _2360 Pf1N1B4_2361 - Malonate decarboxylase alpha subunit, at 2,678,613 to 2,680,211 _2361 Pf1N1B4_2362 - Triphosphoribosyl-dephospho-CoA synthetase (EC 2.7.8.25), at 2,680,211 to 2,681,086 _2362 Pf1N1B4_2363 - Malonate decarboxylase delta subunit, at 2,681,086 to 2,681,385 _2363 Pf1N1B4_2364 - Malonate decarboxylase beta subunit, at 2,681,378 to 2,682,229 _2364
Group Condition Pf1N1B4_2360 Pf1N1B4_2361 Pf1N1B4_2362 Pf1N1B4_2363 Pf1N1B4_2364
temperature LB 37C -1.5 +0.1 -0.8 N.D. -1.0
carbon source carbon source Sucrose 1mM -3.2 +0.2 -0.2 N.D. +0.6
carbon source carbon source Sucrose 1mM -1.3 -0.0 -0.6 N.D. -0.6
carbon source carbon source Sucrose 0.5mM -2.6 +0.1 +0.2 N.D. -0.1
nitrogen source D-Glucosamine Hydrochloride (N) -1.9 -0.2 +0.3 N.D. -0.4
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A -1.2 -0.2 -0.8 N.D. +0.2
carbon source carbon source Sucrose 0.25mM -1.5 -0.0 -0.2 N.D. -0.4
carbon source L-Leucine (C) -1.6 -0.2 -0.1 N.D. -0.1
denitrifying Nitrate reduction, replicate B -1.3 -0.1 -0.6 N.D. +0.0
stress Cholin acetate 10 mM -2.1 -0.1 +0.2 N.D. +0.1
nitrogen source L-Valine (N) -1.8 -0.2 +0.2 N.D. -0.0
pH Minimal media pH9 -1.2 -0.1 -0.0 N.D. -0.5
carbon source D-Glucose (C) -1.0 -0.1 -0.3 N.D. -0.4
stress 1-ethyl-3-methylimidazolium chloride 80 mM -1.6 +0.2 -0.2 N.D. -0.1
carbon source Glycerol (C) -1.5 -0.2 -0.1 N.D. +0.1
stress Rifampicin 0.004 mg/ml -0.4 -0.1 -0.4 N.D. -0.8
stress Tetracycline 0.0004 mg/ml -1.1 +0.2 -0.2 N.D. -0.5
carbon source carbon source Sucrose 0.25mM -1.5 -0.8 +0.4 N.D. +0.4
carbon source D-Fructose (C) -1.6 -0.1 +0.1 N.D. +0.2
carbon source D-Lactate (C) -0.6 +0.1 +0.0 N.D. -0.9
stress Dimethyl Sulfoxide 6.5 vol% -1.3 -0.2 +0.3 N.D. +0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A -0.4 -0.1 +0.3 N.D. -0.8
carbon source carbon source Sucrose 5mM -0.8 -0.3 -0.2 N.D. +0.7
nitrogen source L-Methionine (N) -1.2 +0.0 +0.5 N.D. +0.2
temperature LB 37C +1.1 -0.2 -0.2 N.D. -0.3
denitrifying Nitrate reduction, replicate C +1.0 -0.1 -0.8 N.D. +0.4
anaerobic Nitrate reduction +0.2 -0.2 -0.2 N.D. +1.0
anaerobic Nitrate reduction +0.6 -0.0 -0.2 N.D. +1.1
carbon source carbon source Sucrose 20mM +0.5 +0.4 +0.6 N.D. +0.2
anaerobic Nitrate reduction +1.0 +0.0 -0.2 N.D. +1.6
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Pf1N1B4_2360
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