Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_2357 and Pf1N1B4_2358 are separated by 36 nucleotidesPf1N1B4_2358 and Pf1N1B4_2359 overlap by 8 nucleotidesPf1N1B4_2359 and Pf1N1B4_2360 are separated by 114 nucleotidesPf1N1B4_2360 and Pf1N1B4_2361 are separated by 715 nucleotides Pf1N1B4_2357 - type IV pilus biogenesis protein PilJ, at 2,668,957 to 2,671,014 _2357 Pf1N1B4_2358 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 2,671,051 to 2,676,954 _2358 Pf1N1B4_2359 - Chemotaxis signal transduction protein, at 2,676,947 to 2,677,405 _2359 Pf1N1B4_2360 - hypothetical protein, at 2,677,520 to 2,677,897 _2360 Pf1N1B4_2361 - Malonate decarboxylase alpha subunit, at 2,678,613 to 2,680,211 _2361
Group Condition Pf1N1B4_2357 Pf1N1B4_2358 Pf1N1B4_2359 Pf1N1B4_2360 Pf1N1B4_2361
carbon source carbon source Sucrose 0.25mM -0.2 -0.1 -2.2 -1.5 -0.0
carbon source carbon source Sucrose 0.25mM -0.1 +0.4 -1.4 -1.5 -0.8
carbon source carbon source Sucrose 1mM +0.1 +0.3 -0.6 -3.2 +0.2
temperature LB 37C +0.1 +0.3 -1.7 -1.5 +0.1
carbon source carbon source Sucrose 0.5mM +0.2 +0.4 -0.6 -2.6 +0.1
nitrogen source D-Glucosamine Hydrochloride (N) +0.4 -0.0 -0.6 -1.9 -0.2
stress Cholin acetate 10 mM +0.1 -0.0 -0.1 -2.1 -0.1
stress 1-ethyl-3-methylimidazolium chloride 80 mM -0.2 +0.3 -0.9 -1.6 +0.2
stress Gentamicin 0.001 mg/ml +0.2 -0.5 -1.0 -0.4 -0.2
stress 1-ethyl-3-methylimidazolium lysine 5 mM -0.3 +0.2 -1.2 -0.7 +0.1
pH Minimal media pH9 -0.6 +0.8 -1.4 -0.6 -0.0
pH Minimal media pH9 -0.3 +0.9 -0.8 -1.2 -0.1
nitrogen source D-Alanine (N) +0.4 +0.1 -0.7 -1.3 +0.0
stress Dimethyl Sulfoxide 6.5 vol% +0.1 -0.5 +0.4 -1.3 -0.2
stress Choline Lysine 6 mM -0.3 +0.4 -1.7 +0.2 +0.0
carbon source L-Leucine (C) +0.7 +0.3 -0.5 -1.6 -0.2
carbon source carbon source Sucrose 1mM -0.2 +0.1 +0.4 -1.3 -0.0
carbon source D-Fructose (C) +0.6 -0.1 +0.1 -1.6 -0.1
carbon source Glycerol (C) +0.6 +0.1 -0.0 -1.5 -0.2
stress nitrate 100 mM -0.1 +0.7 -0.9 -0.5 +0.1
nitrogen source L-Valine (N) +1.1 -0.1 +0.3 -1.8 -0.2
carbon source octanoate (C) +1.0 -0.7 -0.0 -0.7 -0.1
stress Cisplatin 0.006 mg/ml +0.1 -0.3 +0.8 -0.8 -0.1
carbon source D-Trehalose (C) +0.4 +0.2 +0.4 -1.1 -0.2
nitrogen source L-Methionine (N) +0.3 +0.2 +0.5 -1.2 +0.0
carbon source Tween 20 (C) +0.8 -0.2 +0.4 -1.0 -0.1
lb LB +0.3 -0.1 +0.5 +0.8 -0.4
denitrifying Nitrate reduction, replicate C t2 +1.1 +0.2 +0.8 -0.6 +0.0
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 +0.9 -0.1 +0.6 +0.5 -0.2
denitrifying Nitrate reduction, replicate C +0.5 +0.3 +1.0 +1.0 -0.1
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