Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_2242 and Pf1N1B4_2243 are separated by 265 nucleotidesPf1N1B4_2243 and Pf1N1B4_2244 are separated by 167 nucleotidesPf1N1B4_2244 and Pf1N1B4_2245 are separated by 369 nucleotidesPf1N1B4_2245 and Pf1N1B4_2246 are separated by 293 nucleotides Pf1N1B4_2242 - Acetylornithine deacetylase (EC 3.5.1.16), at 2,556,986 to 2,558,155 _2242 Pf1N1B4_2243 - N-acetylglutamate synthase (EC 2.3.1.1), at 2,558,421 to 2,559,719 _2243 Pf1N1B4_2244 - Taurine-binding periplasmic protein TauA, at 2,559,887 to 2,560,873 _2244 Pf1N1B4_2245 - Outer membrane porin, OprD family, at 2,561,243 to 2,562,601 _2245 Pf1N1B4_2246 - Alkyl hydroperoxide reductase subunit C-like protein, at 2,562,895 to 2,563,533 _2246
Group Condition Pf1N1B4_2242 Pf1N1B4_2243 Pf1N1B4_2244 Pf1N1B4_2245 Pf1N1B4_2246
carbon source carbon source Sucrose 0.25mM -0.8 N.D. -2.3 -0.1 -0.0
stress Fusidic 0.125 mg/ml -0.7 N.D. -1.7 -0.0 +0.1
carbon source carbon source Sucrose 5mM -0.4 N.D. -1.6 +0.2 -0.1
nitrogen source D-Glucosamine Hydrochloride (N) -0.5 N.D. -1.6 +0.1 +0.1
anaerobic N2E2wt with N1B4m denitrifying repA -0.1 N.D. -1.1 -0.2 -0.1
stress Choline Lysine 6 mM -0.2 N.D. -1.4 -0.0 +0.2
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.5 N.D. -0.7 +0.2 -0.4
stress Gentamicin 0.001 mg/ml -0.3 N.D. -1.0 +0.1 -0.2
denitrifying Nitrate reduction, replicate B +0.1 N.D. -0.9 +0.2 -0.7
carbon source L-Isoleucine (C) -0.4 N.D. -1.0 -0.1 +0.2
stress Cisplatin 0.006 mg/ml -0.3 N.D. -1.1 +0.1 +0.1
anaerobic Nitrate reduction -0.0 N.D. -1.0 +0.2 -0.2
stress nitrite 10 mM +0.1 N.D. -1.3 -0.1 +0.3
stress Sisomicin 0.001 mg/ml -0.0 N.D. -1.2 +0.4 -0.1
carbon source carbon source Sucrose 1mM +0.4 N.D. -1.0 +0.0 -0.2
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A +0.2 N.D. -0.8 -0.2 +0.2
carbon source carbon source Sucrose 0.25mM -0.5 N.D. +0.3 +0.2 -0.5
carbon source carbon source Sucrose 5mM +0.4 N.D. -0.9 -0.1 +0.2
carbon source butyrate (C) -0.7 N.D. +0.3 -0.1 +0.3
carbon source carbon source Sucrose 1mM -0.7 N.D. +0.6 +0.2 -0.1
nitrogen source L-Methionine (N) -0.9 N.D. +0.9 +0.0 +0.2
stress Aluminum chloride 2.5 mM -0.3 N.D. +0.9 +0.0 -0.2
stress copper (II) chloride 2.5 mM +0.2 N.D. +0.9 -0.3 -0.3
carbon source D-Mannose (C) -0.4 N.D. +0.9 -0.0 +0.2
temperature LB 37C -0.2 N.D. +1.1 -0.2 +0.0
nitrogen source Putrescine (N) -0.5 N.D. +1.0 -0.1 +0.4
carbon source Glycerol (C) -0.5 N.D. +0.7 +0.1 +0.5
carbon source Tween 20 (C) +0.3 N.D. +1.0 -0.2 +0.1
stress nitrite 10 mM +0.6 N.D. +0.3 -0.1 +0.4
stress sodium fluoride 12.5 mM +0.8 N.D. +0.6 +0.1 +0.1
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