Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_2152 and Pf1N1B4_2153 are separated by 108 nucleotidesPf1N1B4_2153 and Pf1N1B4_2154 overlap by 23 nucleotidesPf1N1B4_2154 and Pf1N1B4_2155 are separated by 21 nucleotidesPf1N1B4_2155 and Pf1N1B4_2156 are separated by 184 nucleotides Pf1N1B4_2152 - GGDEF domain/EAL domain protein, at 2,463,712 to 2,465,385 _2152 Pf1N1B4_2153 - Multidrug and toxin extrusion (MATE) family efflux pump YdhE/NorM, homolog, at 2,465,494 to 2,466,891 _2153 Pf1N1B4_2154 - hypothetical protein, at 2,466,869 to 2,467,027 _2154 Pf1N1B4_2155 - Glycine cleavage system transcriptional activator, at 2,467,049 to 2,467,969 _2155 Pf1N1B4_2156 - Methyl-accepting chemotaxis protein, at 2,468,154 to 2,470,130 _2156
Group Condition Pf1N1B4_2152 Pf1N1B4_2153 Pf1N1B4_2154 Pf1N1B4_2155 Pf1N1B4_2156
anaerobic N2E2wt with N1B4m denitrifying repA N.D. +0.1 -2.7 -0.1 -0.1
carbon source carbon source Sucrose 1mM N.D. -0.1 +0.1 -1.6 -0.2
temperature LB 37C N.D. +0.3 -1.0 -0.8 +0.1
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 N.D. +0.2 -1.4 -0.4 +0.1
carbon source carbon source Sucrose 0.25mM N.D. +0.1 -1.8 +0.3 -0.1
stress Doxycycline hyclate 0.0001 mg/ml N.D. +0.1 -1.7 +0.3 -0.1
carbon source D-Ribose (C) N.D. -0.1 -0.9 -0.4 +0.0
carbon source carbon source Sucrose 5mM N.D. -0.0 -0.9 -0.5 +0.2
stress Spectinomycin 0.05 mg/ml N.D. +0.2 -0.5 -0.8 -0.2
stress Bacitracin 2 mg/ml N.D. +0.2 -1.4 +0.2 -0.1
denitrifying Nitrate reduction, replicate C N.D. +0.0 -1.0 +0.1 -0.3
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 N.D. -0.0 -1.2 +0.2 +0.4
stress Cobalt chloride 0.3125 mM N.D. +0.3 -1.2 +0.3 +0.1
stress Choline chloride 200 mM N.D. +0.0 +0.4 -1.1 +0.2
stress Chloride 300 mM N.D. +0.2 -0.6 -0.4 +0.5
nitrogen source nitrogen source nitrite 1mM N.D. +0.4 +0.2 -0.8 -0.0
stress Gentamicin 0.001 mg/ml N.D. -0.1 +0.5 -0.6 +0.3
stress Thallium(I) acetate 0.008 mg/ml N.D. -0.1 +0.8 -0.5 +0.1
stress 1-ethyl-3-methylimidazolium lysine 5 mM N.D. -0.4 +1.1 -0.5 +0.1
anaerobic N2E2wt with N1B4m denitrifying repA N.D. +0.1 +0.8 -0.4 -0.1
nitrogen source nitrogen source nitrite 0.1mM N.D. +0.2 +0.7 -0.5 +0.0
stress Aluminum chloride 2.5 mM N.D. -0.5 +0.5 +0.6 +0.0
temperature LB 37C N.D. -0.3 +0.8 +0.3 +0.1
stress Cholin acetate 20 mM N.D. -0.1 +1.0 -0.2 +0.2
nitrogen source nitrogen source nitrite 0.1mM N.D. -0.1 +0.7 +0.2 +0.3
carbon source carbon source Sucrose 5mM N.D. +0.0 +1.6 -0.4 -0.2
carbon source L-Leucine (C) N.D. -0.1 +0.8 +0.5 +0.0
pH Minimal media pH9 N.D. +0.1 +1.1 +0.4 -0.1
stress nitrite 10 mM N.D. +0.1 +0.7 +0.4 +0.3
nitrogen source Cytidine (N) N.D. +0.0 +1.6 +0.3 -0.0
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