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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_2037 and Pf1N1B4_2038 are separated by 120 nucleotides
Pf1N1B4_2038 and Pf1N1B4_2039 are separated by 50 nucleotides
Pf1N1B4_2039 and Pf1N1B4_2040 are separated by 11 nucleotides
Pf1N1B4_2040 and Pf1N1B4_2041 are separated by 201 nucleotides
Pf1N1B4_2037 - Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1), at 2,326,197 to 2,327,030
_2037
Pf1N1B4_2038 - Phosphate transport system regulatory protein PhoU, at 2,327,151 to 2,327,912
_2038
Pf1N1B4_2039 - hypothetical protein, at 2,327,963 to 2,328,082
_2039
Pf1N1B4_2040 - Phosphate regulon transcriptional regulatory protein PhoB (SphR), at 2,328,094 to 2,329,044
_2040
Pf1N1B4_2041 - Peptidase, M23/M37 family, at 2,329,246 to 2,330,142
_2041
Group
Condition
Pf1N1B4
_2037
Pf1N1B4
_2038
Pf1N1B4
_2039
Pf1N1B4
_2040
Pf1N1B4
_2041
temperature
LB 10C
-2.8
N.D.
-0.7
N.D.
+0.0
carbon source
carbon source Sucrose 1mM
+0.5
N.D.
-3.1
N.D.
+0.1
stress
Cholin acetate 10 mM
-1.2
N.D.
-1.3
N.D.
+0.1
nitrogen source
nitrogen source nitrite 0.1mM
-0.3
N.D.
-1.3
N.D.
-0.6
stress
Thallium(I) acetate 0.008 mg/ml
-1.5
N.D.
-0.4
N.D.
-0.3
anaerobic
N2E2wt with N1B4m denitrifying repB
-0.4
N.D.
-1.6
N.D.
-0.0
stress
Bacitracin 2 mg/ml
-2.5
N.D.
+0.5
N.D.
-0.0
stress
Cisplatin 0.006 mg/ml
-1.5
N.D.
-0.5
N.D.
+0.0
nitrogen source
Ammonium chloride (N)
-0.5
N.D.
-0.8
N.D.
-0.6
stress
Spectinomycin 0.05 mg/ml
-2.1
N.D.
+0.2
N.D.
-0.1
carbon source
carbon source Sucrose 5mM
-1.4
N.D.
-0.4
N.D.
+0.1
carbon source
D-Ribose (C)
+0.2
N.D.
-1.3
N.D.
-0.5
stress
5-Hydroxymethylfurfural 2.5 mM
-0.7
N.D.
-1.1
N.D.
+0.2
stress
Rifampicin 0.004 mg/ml
-1.4
N.D.
-0.3
N.D.
+0.2
stress
Nalidixic 0.004 mg/ml
-1.6
N.D.
-0.1
N.D.
+0.2
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
-0.7
N.D.
+0.1
N.D.
-0.9
stress
Spectinomycin 0.025 mg/ml
-1.5
N.D.
+0.2
N.D.
-0.2
phagefrs
Time16-FRS
-1.5
N.D.
+0.2
N.D.
-0.1
nitrogen source
nitrogen source nitrite 0.5mM
-1.2
N.D.
+0.6
N.D.
-0.5
carbon source
Glycerol (C)
-1.2
N.D.
+0.5
N.D.
-0.3
stress
Chloride 300 mM
-0.1
N.D.
-1.5
N.D.
+0.6
stress
Sisomicin 0.001 mg/ml
-1.7
N.D.
+0.4
N.D.
+0.4
anaerobic
Nitrate reduction
+0.4
N.D.
-1.1
N.D.
-0.2
carbon source
octanoate (C)
-2.0
N.D.
+1.1
N.D.
+0.1
pH
Minimal media pH7
-1.2
N.D.
+0.5
N.D.
+0.2
pH
Minimal media pH7
-1.4
N.D.
+0.8
N.D.
+0.2
stress
Cephalothin 2.5 mg/ml
-0.8
N.D.
+0.8
N.D.
-0.3
carbon source
L-Alanine (C)
-0.4
N.D.
+1.1
N.D.
-0.6
nitrogen source
L-Isoleucine (N)
-0.6
N.D.
+1.1
N.D.
-0.3
stress
Gentamicin 0.001 mg/ml
-0.9
N.D.
+0.9
N.D.
+0.3
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