Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_2024 and Pf1N1B4_2025 are separated by 538 nucleotidesPf1N1B4_2025 and Pf1N1B4_2026 are separated by 180 nucleotidesPf1N1B4_2026 and Pf1N1B4_2027 are separated by 334 nucleotidesPf1N1B4_2027 and Pf1N1B4_2028 are separated by 11 nucleotides Pf1N1B4_2024 - sodium/alanine transporter, at 2,309,825 to 2,311,276 _2024 Pf1N1B4_2025 - Aspartate ammonia-lyase (EC 4.3.1.1), at 2,311,815 to 2,313,239 _2025 Pf1N1B4_2026 - Transcriptional regulator, at 2,313,420 to 2,314,337 _2026 Pf1N1B4_2027 - Phosphoribosylaminoimidazole carboxylase catalytic subunit (EC 4.1.1.21), at 2,314,672 to 2,315,163 _2027 Pf1N1B4_2028 - Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21), at 2,315,175 to 2,316,257 _2028
Group Condition Pf1N1B4_2024 Pf1N1B4_2025 Pf1N1B4_2026 Pf1N1B4_2027 Pf1N1B4_2028
carbon source L-Asparagine (C) -1.7 N.D. -1.8 N.D. N.D.
temperature LB 37C -0.2 N.D. -3.0 N.D. N.D.
carbon source carbon source Sucrose 1mM -0.5 N.D. -2.5 N.D. N.D.
carbon source L-Glutamic (C) -0.8 N.D. -2.0 N.D. N.D.
stress Benzethonium chloride 0.0125 mM -0.3 N.D. -2.3 N.D. N.D.
carbon source octanoate (C) -0.2 N.D. -2.3 N.D. N.D.
carbon source carbon source Sucrose 0.25mM -0.1 N.D. -2.3 N.D. N.D.
carbon source carbon source Sucrose 1mM -0.5 N.D. -1.8 N.D. N.D.
carbon source L-Arginine (C) +0.1 N.D. -2.2 N.D. N.D.
stress Rifampicin 0.004 mg/ml +0.1 N.D. -2.1 N.D. N.D.
stress Thallium(I) acetate 0.008 mg/ml -0.3 N.D. -1.4 N.D. N.D.
stress Thallium(I) acetate 0.008 mg/ml -0.1 N.D. -1.6 N.D. N.D.
stress Doxycycline hyclate 0.0001 mg/ml -0.2 N.D. -1.4 N.D. N.D.
stress Cisplatin 0.006 mg/ml -0.2 N.D. -1.4 N.D. N.D.
stress Spectinomycin 0.05 mg/ml -0.3 N.D. -1.2 N.D. N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A -0.1 N.D. -1.3 N.D. N.D.
denitrifying Nitrate reduction, replicate C -0.1 N.D. -1.3 N.D. N.D.
carbon source carbon source Sucrose 0.25mM +0.0 N.D. -1.4 N.D. N.D.
lb LB -0.0 N.D. -1.2 N.D. N.D.
temperature LB 30C -0.3 N.D. -1.0 N.D. N.D.
stress Cobalt chloride 0.3125 mM +0.4 N.D. -1.5 N.D. N.D.
stress Paraquat dichloride 0.016 mg/mL +0.4 N.D. -1.5 N.D. N.D.
nitrogen source nitrogen source nitrite 0.1mM +0.2 N.D. -1.3 N.D. N.D.
nitrogen source nitrogen source Ammonium chloride 20mM +0.1 N.D. -1.1 N.D. N.D.
carbon source L-Glutamine (C) +0.2 N.D. -1.0 N.D. N.D.
carbon source L-Valine (C) +1.0 N.D. -0.3 N.D. N.D.
pH Minimal media pH9 -1.0 N.D. +2.4 N.D. N.D.
pH Minimal media pH9 -0.9 N.D. +2.3 N.D. N.D.
nitrogen source L-Serine (N) +0.7 N.D. +0.8 N.D. N.D.
stress Carbenicillin 2 mg/ml +0.5 N.D. +1.8 N.D. N.D.
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