Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_1991 and Pf1N1B4_1992 overlap by 4 nucleotidesPf1N1B4_1992 and Pf1N1B4_1993 are separated by 127 nucleotidesPf1N1B4_1993 and Pf1N1B4_1994 are separated by 28 nucleotidesPf1N1B4_1994 and Pf1N1B4_1995 overlap by 1 nucleotides Pf1N1B4_1991 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 2,276,193 to 2,277,401 _1991 Pf1N1B4_1992 - Glutamine synthetase adenylyltransferase, at 2,277,398 to 2,277,775 _1992 Pf1N1B4_1993 - C4-type zinc finger protein, DksA/TraR family, at 2,277,903 to 2,278,307 _1993 Pf1N1B4_1994 - Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family, at 2,278,336 to 2,279,544 _1994 Pf1N1B4_1995 - FIG00954044: hypothetical protein, at 2,279,544 to 2,280,194 _1995
Group Condition Pf1N1B4_1991 Pf1N1B4_1992 Pf1N1B4_1993 Pf1N1B4_1994 Pf1N1B4_1995
temperature LB 37C -0.1 +0.1 -3.5 -0.7 +1.0
anaerobic N2E2wt with N1B4m denitrifying repB -0.2 -0.0 -2.3 -0.6 +0.1
denitrifying Nitrate reduction, replicate C t2 +0.2 -0.2 -1.6 -0.7 -0.7
carbon source carbon source Sucrose 1mM +1.1 -0.9 -1.9 -0.2 -0.2
denitrifying Nitrate reduction, replicate C t1 +0.4 +0.0 -1.6 -0.4 -0.5
denitrifying Nitrate reduction, replicate C t3 +0.2 -0.0 -1.5 -0.2 -0.5
carbon source m-Inositol (C) +0.1 -0.5 -0.8 -0.5 -0.4
stress copper (II) chloride 2.5 mM -0.4 +0.2 -1.7 -0.4 +0.4
nitrogen source nitrogen source nitrite 0.1mM +0.0 -0.2 -1.6 -0.2 +0.1
stress 1-ethyl-3-methylimidazolium lysine 5 mM +0.2 -0.4 -0.5 -0.5 -0.5
stress Rifampicin 0.004 mg/ml +0.5 -0.0 -1.6 -0.2 -0.4
carbon source carbon source Sucrose 0.5mM +0.9 -0.4 -2.5 +0.2 +0.1
pH Minimal media pH7 +0.4 +0.2 -1.6 -0.4 -0.3
temperature LB 37C +0.2 +0.3 -2.3 -0.2 +0.4
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.3 -0.4 +0.4 -0.2 -0.8
stress Tetracycline 0.0004 mg/ml +0.4 -0.2 -1.2 -0.8 +0.5
denitrifying Nitrate reduction, replicate A t2 +0.1 +0.0 -1.7 +0.1 +0.3
anaerobic Nitrate reduction +0.3 +0.2 -0.1 -0.8 -0.8
carbon source carbon source Sucrose 5mM +1.1 -0.5 -0.9 -0.0 -0.8
carbon source D-Fructose (C) +0.3 +0.4 -1.2 -0.3 -0.1
carbon source carbon source Sucrose 1mM +0.9 +0.9 -1.3 -0.5 -0.6
carbon source carbon source Sucrose 5mM +0.0 +0.4 -1.5 +0.1 +0.3
pH Minimal media pH9 +0.6 -0.2 -1.8 +0.2 +0.8
nitrogen source L-Isoleucine (N) +0.0 -0.3 +0.9 -0.6 -0.2
stress Sisomicin 0.001 mg/ml +0.4 +0.3 -1.1 +0.3 -0.2
carbon source octanoate (C) +0.3 -0.1 +0.9 -0.7 -0.6
carbon source carbon source Sucrose 0.25mM +0.1 +0.6 +0.5 -1.0 -0.1
phagefrs Time16-FRS +0.2 -0.0 +1.5 -0.1 -0.4
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A +0.3 -0.1 +1.6 -0.4 +0.1
stress Cisplatin 0.006 mg/ml +0.1 +0.1 +1.3 +0.8 +0.3
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