Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_5190 and Pf1N1B4_5191 are separated by 81 nucleotidesPf1N1B4_5191 and Pf1N1B4_1815 are separated by 650 nucleotidesPf1N1B4_1815 and Pf1N1B4_1816 are separated by 13 nucleotidesPf1N1B4_1816 and Pf1N1B4_1817 are separated by 158 nucleotides Pf1N1B4_5190 - tRNA-Ile-GAT, at 2,089,845 to 2,089,918 _5190 Pf1N1B4_5191 - Small Subunit Ribosomal RNA; ssuRNA; SSU rRNA, at 2,090,000 to 2,091,528 _5191 Pf1N1B4_1815 - L-carnitine dehydratase/bile acid-inducible protein F, at 2,092,179 to 2,093,399 _1815 Pf1N1B4_1816 - Glutaryl-CoA dehydrogenase (EC 1.3.99.7), at 2,093,413 to 2,094,594 _1816 Pf1N1B4_1817 - Glycine cleavage system transcriptional activator, at 2,094,753 to 2,095,652 _1817
Group Condition Pf1N1B4_5190 Pf1N1B4_5191 Pf1N1B4_1815 Pf1N1B4_1816 Pf1N1B4_1817
nitrogen source L-Lysine (N) N.D. N.D. -0.5 -0.7 -0.5
carbon source L-Valine (C) N.D. N.D. -0.7 -0.8 +0.0
carbon source L-Leucine (C) N.D. N.D. -0.3 -0.7 -0.5
carbon source carbon source Sucrose 1mM N.D. N.D. -0.7 -0.7 +0.2
carbon source carbon source Sucrose 0.5mM N.D. N.D. -0.6 -0.5 -0.1
carbon source carbon source Sucrose 1mM N.D. N.D. -0.5 -0.7 +0.2
stress Rifampicin 0.004 mg/ml N.D. N.D. -0.3 -0.3 -0.1
stress Cobalt chloride 0.3125 mM N.D. N.D. -0.5 -0.1 -0.2
carbon source pyruvate (C) N.D. N.D. -0.1 -0.3 -0.2
carbon source carbon source Sucrose 5mM N.D. N.D. -0.2 -0.2 -0.2
anaerobic N2E2wt with N1B4m denitrifying repA N.D. N.D. -0.2 +0.1 -0.4
carbon source casaminos (C) N.D. N.D. -0.2 +0.2 -0.4
stress Cephalothin 2.5 mg/ml N.D. N.D. -0.3 +0.1 -0.3
carbon source carbon source Sucrose 0.25mM N.D. N.D. -0.4 -0.2 +0.2
carbon source carbon source Sucrose 0.25mM N.D. N.D. -0.6 +0.0 +0.1
stress Paraquat dichloride 0.016 mg/mL N.D. N.D. -0.1 +0.2 -0.3
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 N.D. N.D. +0.4 -0.5 -0.0
carbon source L-Isoleucine (C) N.D. N.D. -0.4 +0.2 +0.0
carbon source D-Alanine (C) N.D. N.D. +0.1 +0.3 -0.3
nitrogen source L-Methionine (N) N.D. N.D. +0.1 +0.4 -0.3
nitrogen source nitrate (N) N.D. N.D. +0.2 +0.3 -0.2
anaerobic Nitrate reduction N.D. N.D. +0.4 -0.3 +0.1
denitrifying Nitrate reduction, replicate C t2 N.D. N.D. +0.3 +0.2 +0.2
stress Fusidic 0.0625 mg/ml N.D. N.D. +0.2 +0.4 +0.1
temperature LB 37C N.D. N.D. +0.5 +0.2 +0.0
denitrifying Nitrate reduction, replicate A t1 N.D. N.D. +0.3 +0.4 +0.2
denitrifying Nitrate reduction, replicate A t2 N.D. N.D. +0.6 +0.3 +0.0
stress Cisplatin 0.006 mg/ml N.D. N.D. +0.3 +0.3 +0.4
stress Cisplatin 0.006 mg/ml N.D. N.D. +0.1 +0.7 +0.3
temperature LB 37C N.D. N.D. +0.6 +0.3 +0.2
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