Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_1744 and Pf1N1B4_1745 are separated by 12 nucleotidesPf1N1B4_1745 and Pf1N1B4_1746 are separated by 418 nucleotidesPf1N1B4_1746 and Pf1N1B4_1747 are separated by 181 nucleotidesPf1N1B4_1747 and Pf1N1B4_1748 are separated by 5 nucleotides Pf1N1B4_1744 - Biofilm PGA synthesis deacetylase PgaB (EC 3.-), at 1,993,461 to 1,995,416 _1744 Pf1N1B4_1745 - Biofilm PGA outer membrane secretin PgaA, at 1,995,429 to 1,997,909 _1745 Pf1N1B4_1746 - GTP-binding protein, at 1,998,328 to 1,999,803 _1746 Pf1N1B4_1747 - hypothetical protein, at 1,999,985 to 2,000,116 _1747 Pf1N1B4_1748 - NADH:ubiquinone oxidoreductase subunit 2 (chain N), at 2,000,122 to 2,000,757 _1748
Group Condition Pf1N1B4_1744 Pf1N1B4_1745 Pf1N1B4_1746 Pf1N1B4_1747 Pf1N1B4_1748
carbon source carbon source Sucrose 5mM -0.5 -0.1 -1.5 N.D. -0.3
carbon source carbon source Sucrose 1mM -0.3 -0.6 -0.6 N.D. -0.0
carbon source carbon source Sucrose 1mM +0.4 -0.2 -0.6 N.D. -1.0
carbon source carbon source Sucrose 0.25mM -0.1 -0.0 -0.1 N.D. -0.9
nitrogen source nitrogen source nitrate 20mM +0.0 -0.3 +0.0 N.D. -0.7
pH Minimal media pH9 +0.1 +0.2 -0.5 N.D. -0.7
denitrifying Nitrate reduction, replicate B +0.0 -0.1 -0.5 N.D. -0.4
stress Rifampicin 0.004 mg/ml -0.2 +0.0 +0.2 N.D. -0.8
carbon source carbon source Sucrose 0.5mM +0.2 -0.3 +0.1 N.D. -0.6
nitrogen source nitrogen source Ammonium chloride 20mM -0.2 -0.2 -0.5 N.D. +0.2
stress 1-ethyl-3-methylimidazolium lysine 5 mM -0.3 +0.1 +0.1 N.D. -0.4
temperature LB 37C -0.1 -0.2 -0.5 N.D. +0.3
stress Carbenicillin 2 mg/ml -0.1 +0.2 -0.3 N.D. -0.3
stress acetate 20 mM -0.2 -0.1 +0.3 N.D. -0.4
carbon source L-Citrulline (C) -0.3 +0.1 +0.2 N.D. -0.4
carbon source carbon source Sucrose 5mM +0.1 -0.3 -0.3 N.D. +0.4
carbon source octanoate (C) -0.0 +0.1 +0.6 N.D. -0.8
stress Paraquat dichloride 0.016 mg/mL +0.1 -0.1 +0.4 N.D. -0.5
carbon source L-Glutamine (C) -0.3 +0.2 +0.3 N.D. -0.2
stress Chloride 300 mM -0.3 +0.0 +0.6 N.D. -0.3
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.3 +0.1 -0.4 N.D. +0.7
carbon source L-Alanine (C) -0.1 +0.3 +0.2 N.D. -0.4
stress Doxycycline hyclate 0.0001 mg/ml -0.1 +0.1 +0.4 N.D. -0.4
anaerobic Nitrate reduction -0.2 -0.3 +0.4 N.D. +0.2
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 +0.6 +0.2 -0.1 N.D. -0.3
stress Benzethonium chloride 0.0125 mM -0.0 -0.1 +0.7 N.D. -0.2
stress Aluminum chloride 2.5 mM +0.0 +0.2 -0.4 N.D. +0.5
denitrifying Nitrate reduction, replicate C +0.2 +0.1 +0.6 N.D. -0.4
stress Fusidic 0.125 mg/ml -0.2 +0.1 +1.0 N.D. -0.3
stress Fusidic 0.0625 mg/ml +0.0 +0.2 +0.7 N.D. -0.3
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