Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_1684 and Pf1N1B4_1685 are separated by 165 nucleotidesPf1N1B4_1685 and Pf1N1B4_1686 are separated by 370 nucleotidesPf1N1B4_1686 and Pf1N1B4_1687 are separated by 125 nucleotidesPf1N1B4_1687 and Pf1N1B4_1688 are separated by 170 nucleotides Pf1N1B4_1684 - Oxidoreductase, aldo/keto reductase family, at 1,930,965 to 1,932,002 _1684 Pf1N1B4_1685 - Antitoxin 1, at 1,932,168 to 1,932,569 _1685 Pf1N1B4_1686 - Transcriptional regulator, LysR family, at 1,932,940 to 1,933,830 _1686 Pf1N1B4_1687 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 1,933,956 to 1,934,696 _1687 Pf1N1B4_1688 - hypothetical protein, at 1,934,867 to 1,935,127 _1688
Group Condition Pf1N1B4_1684 Pf1N1B4_1685 Pf1N1B4_1686 Pf1N1B4_1687 Pf1N1B4_1688
pH Minimal media pH9 -0.0 N.D. -0.7 -0.7 N.D.
denitrifying Nitrate reduction, replicate C -0.5 N.D. -0.4 -0.3 N.D.
carbon source D-Mannose (C) -1.4 N.D. +0.1 +0.2 N.D.
denitrifying Nitrate reduction, replicate A -0.3 N.D. -0.4 -0.6 N.D.
carbon source carbon source Sucrose 0.25mM -0.5 N.D. -0.7 +0.1 N.D.
carbon source carbon source Sucrose 0.5mM +0.2 N.D. +0.0 -1.3 N.D.
stress 1-ethyl-3-methylimidazolium chloride 80 mM -0.0 N.D. -0.4 -0.6 N.D.
stress 1-ethyl-3-methylimidazolium lysine 5 mM -0.3 N.D. -0.5 -0.1 N.D.
stress Carbenicillin 2 mg/ml -0.4 N.D. -0.1 -0.3 N.D.
stress Spectinomycin 0.05 mg/ml +0.1 N.D. -0.6 -0.2 N.D.
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 -0.3 N.D. -0.0 -0.3 N.D.
lb LB -0.2 N.D. +0.1 -0.5 N.D.
carbon source carbon source Sucrose 1mM +0.3 N.D. -0.4 -0.5 N.D.
stress Benzethonium chloride 0.0125 mM +0.3 N.D. -0.3 -0.5 N.D.
nitrogen source Cytidine (N) +0.1 N.D. -0.5 -0.2 N.D.
temperature LB 37C -0.3 N.D. +0.3 -0.4 N.D.
denitrifying Nitrate reduction, replicate B t2 -0.1 N.D. -0.4 +0.1 N.D.
stress Dimethyl Sulfoxide 5 vol% +0.0 N.D. +0.1 -0.5 N.D.
stress Chloride 300 mM -0.3 N.D. -0.3 +0.2 N.D.
stress D-Cycloserine 0.1 mg/ml -0.3 N.D. -0.2 +0.2 N.D.
carbon source carbon source Sucrose 0.25mM -0.2 N.D. -0.2 +0.2 N.D.
carbon source carbon source Sucrose 1mM -0.2 N.D. -0.5 +0.4 N.D.
carbon source carbon source Sucrose 5mM +0.3 N.D. +0.1 -0.6 N.D.
stress nitrate 100 mM -0.0 N.D. +0.5 -0.4 N.D.
denitrifying Nitrate reduction, replicate A t1 -0.1 N.D. -0.2 +0.5 N.D.
nitrogen source nitrogen source nitrite 1mM +0.2 N.D. -0.2 +0.3 N.D.
denitrifying Nitrate reduction, replicate A t2 -0.2 N.D. +0.2 +0.4 N.D.
stress Paraquat dichloride 0.016 mg/mL -0.2 N.D. +0.4 +0.4 N.D.
carbon source Fumarate (C) +0.1 N.D. +0.3 +0.4 N.D.
carbon source carbon source Sucrose 5mM +0.1 N.D. -0.1 +0.9 N.D.
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