Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_1661 and Pf1N1B4_1662 are separated by 3 nucleotidesPf1N1B4_1662 and Pf1N1B4_1663 are separated by 329 nucleotidesPf1N1B4_1663 and Pf1N1B4_1664 are separated by 886 nucleotidesPf1N1B4_1664 and Pf1N1B4_1665 are separated by 160 nucleotides Pf1N1B4_1661 - Agmatine deiminase (EC 3.5.3.12), at 1,901,848 to 1,902,954 _1661 Pf1N1B4_1662 - N-carbamoylputrescine amidase (3.5.1.53) / Aliphatic amidase AmiE (EC 3.5.1.4), at 1,902,958 to 1,903,836 _1662 Pf1N1B4_1663 - 4-aminobutyrate aminotransferase (EC 2.6.1.19), at 1,904,166 to 1,907,078 _1663 Pf1N1B4_1664 - dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13), at 1,907,965 to 1,908,510 _1664 Pf1N1B4_1665 - Signal transduction histidine kinase regulating C4-dicarboxylate transport system, at 1,908,671 to 1,910,425 _1665
Group Condition Pf1N1B4_1661 Pf1N1B4_1662 Pf1N1B4_1663 Pf1N1B4_1664 Pf1N1B4_1665
temperature LB 37C +0.2 -0.4 -0.2 -1.4 -0.0
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.2 -1.4 -0.1 +0.0 -0.1
stress Gentamicin 0.001 mg/ml -2.1 +0.1 -0.0 +0.1 +0.2
stress Carbenicillin 2 mg/ml -0.8 -0.4 -0.1 -0.0 -0.3
nitrogen source Cytidine (N) -0.8 -0.5 +0.1 -0.4 +0.0
carbon source L-Leucine (C) -0.7 -0.2 +0.2 -0.4 -0.3
stress Paraquat dichloride 0.016 mg/mL -1.5 -0.0 +0.1 -0.2 +0.1
pH Minimal media pH9 -0.6 -0.1 -0.0 +0.0 -0.8
pH Minimal media pH9 -0.9 +0.1 -0.0 -0.2 -0.2
carbon source carbon source Sucrose 1mM -0.4 -0.2 -0.3 -0.5 +0.2
nitrogen source L-Methionine (N) -1.7 +0.3 +0.2 +0.1 -0.1
stress Cisplatin 0.006 mg/ml -1.4 -0.1 +0.0 -0.1 +0.3
nitrogen source nitrogen source nitrate 20mM -0.4 -0.3 -0.2 +0.2 -0.4
stress Cobalt chloride 0.3125 mM -0.7 -0.1 +0.2 -0.5 +0.1
nitrogen source Uridine (N) -0.8 -0.5 +0.3 +0.0 -0.0
carbon source octanoate (C) -0.9 -0.4 +0.3 +0.1 -0.2
stress Cisplatin 0.006 mg/ml -1.0 -0.2 -0.0 +0.0 +0.3
carbon source a-Ketoglutaric (C) -0.6 -0.4 +0.0 -0.4 +0.6
stress Sisomicin 0.001 mg/ml -0.8 -0.4 -0.1 +0.2 +0.5
carbon source Uridine (C) -0.9 -0.2 +0.1 +0.2 +0.3
stress Spectinomycin 0.05 mg/ml -0.8 +0.0 -0.2 +0.1 +0.3
carbon source carbon source Sucrose 0.25mM +0.4 -0.0 -0.3 -0.4 +0.3
stress Doxycycline hyclate 0.0001 mg/ml -0.3 -1.0 +0.2 +0.3 +0.9
stress Chloride 300 mM -0.7 +0.3 +0.0 +0.3 +0.3
carbon source carbon source Sucrose 0.25mM +0.5 +0.4 -0.1 -0.0 -0.4
anaerobic Nitrate reduction +0.6 +0.2 -0.1 -0.3 +0.2
nitrogen source nitrogen source nitrite 0.1mM +0.7 -0.0 -0.0 +0.4 -0.3
stress Nalidixic 0.004 mg/ml +0.4 +0.5 -0.1 +0.2 +0.3
stress Choline Lysine 6 mM +0.5 +0.4 -0.2 +0.3 +0.4
stress Fusidic 0.0625 mg/ml +0.8 +0.3 +0.1 +0.5 +0.5
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