Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_149 and Pf1N1B4_150 are separated by 304 nucleotidesPf1N1B4_150 and Pf1N1B4_151 are separated by 42 nucleotidesPf1N1B4_151 and Pf1N1B4_152 are separated by 274 nucleotidesPf1N1B4_152 and Pf1N1B4_153 are separated by 213 nucleotides Pf1N1B4_149 - Pyoverdine sidechain non-ribosomal peptide synthetase PvdD, at 188,375 to 191,797 _149 Pf1N1B4_150 - SyrP-like protein, at 192,102 to 193,085 _150 Pf1N1B4_151 - Thioesterase in siderophore biosynthesis gene cluster, at 193,128 to 193,868 _151 Pf1N1B4_152 - ABC transporter, permease protein, at 194,143 to 195,264 _152 Pf1N1B4_153 - Glucoamylase (EC 3.2.1.3), at 195,478 to 197,301 _153
Group Condition Pf1N1B4_149 Pf1N1B4_150 Pf1N1B4_151 Pf1N1B4_152 Pf1N1B4_153
carbon source carbon source Sucrose 0.25mM -0.9 +0.4 N.D. -1.9 -0.4
stress Carbenicillin 2 mg/ml -0.4 -0.2 N.D. -1.5 -0.2
denitrifying Nitrate reduction, replicate A t2 +0.2 -0.7 N.D. -0.7 -0.5
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.2 -0.7 N.D. +0.1 -0.8
anaerobic N2E2wt with N1B4m denitrifying repB -0.0 -0.6 N.D. -0.2 -0.7
lb LB -0.6 -0.1 N.D. -0.8 +0.0
temperature LB 37C -0.0 -1.1 N.D. -0.3 +0.0
anaerobic Nitrate reduction +0.0 -0.2 N.D. +0.1 -1.2
stress Gentamicin 0.001 mg/ml -0.5 -0.3 N.D. +0.1 -0.4
carbon source carbon source Sucrose 0.5mM -0.4 +0.4 N.D. -0.8 -0.5
carbon source D-Fructose (C) +0.2 -0.4 N.D. -0.8 -0.2
stress copper (II) chloride 2.5 mM -0.3 -0.5 N.D. -0.3 +0.4
nitrogen source nitrogen source nitrite 0.1mM -0.6 +0.9 N.D. -1.1 +0.2
nophagecontrol TIme6-nophage -0.6 -0.3 N.D. +0.3 +0.2
carbon source carbon source Sucrose 0.25mM -0.1 +1.0 N.D. -0.4 -0.9
denitrifying Nitrate reduction, replicate B +0.3 -0.7 N.D. +0.2 -0.2
temperature LB 37C +0.8 -0.5 N.D. -0.4 -0.1
carbon source D-Glucose (C) +0.4 -0.4 N.D. -0.5 +0.4
nitrogen source nitrogen source nitrate 20mM +0.1 +0.6 N.D. -0.7 +0.0
stress D-Cycloserine 0.1 mg/ml +0.2 -0.5 N.D. +0.6 -0.1
stress Fusidic 0.125 mg/ml +0.2 -0.6 N.D. +0.6 +0.1
lb LB +0.1 +0.0 N.D. +0.7 -0.6
pH Minimal media pH9 +0.2 -0.5 N.D. -0.3 +0.8
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A +0.1 +0.7 N.D. -0.5 -0.2
carbon source carbon source Sucrose 1mM +0.1 +0.9 N.D. +0.0 -0.6
carbon source L-Glutamic (C) +0.0 -0.6 N.D. +0.8 +0.1
carbon source carbon source Sucrose 1mM -0.4 +0.9 N.D. -0.3 +0.3
stress Doxycycline hyclate 0.0001 mg/ml +1.2 -0.3 N.D. -0.4 +0.1
stress Rifampicin 0.004 mg/ml +0.4 +0.6 N.D. -0.5 +0.3
stress Chloride 300 mM -0.1 +0.6 N.D. +0.4 +0.3
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