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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_1435 and Pf1N1B4_1436 are separated by 249 nucleotides
Pf1N1B4_1436 and Pf1N1B4_1437 are separated by 176 nucleotides
Pf1N1B4_1437 and Pf1N1B4_1438 are separated by 10 nucleotides
Pf1N1B4_1438 and Pf1N1B4_1439 overlap by 4 nucleotides
Pf1N1B4_1435 - HAMP domain protein, at 1,645,080 to 1,646,606
_1435
Pf1N1B4_1436 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 1,646,856 to 1,647,569
_1436
Pf1N1B4_1437 - Topoisomerase IV subunit A (EC 5.99.1.-), at 1,647,746 to 1,650,013
_1437
Pf1N1B4_1438 - Transporter, at 1,650,024 to 1,650,545
_1438
Pf1N1B4_1439 - FIG00953138: hypothetical protein, at 1,650,542 to 1,651,531
_1439
Group
Condition
Pf1N1B4
_1435
Pf1N1B4
_1436
Pf1N1B4
_1437
Pf1N1B4
_1438
Pf1N1B4
_1439
nitrogen source
nitrogen source Ammonium chloride 20mM
N.D.
N.D.
N.D.
N.D.
-0.7
carbon source
L-Arabinose (C)
N.D.
N.D.
N.D.
N.D.
-0.7
denitrifying
Nitrate reduction, replicate C
N.D.
N.D.
N.D.
N.D.
-0.6
nitrogen source
nitrogen source Ammonium chloride 20mM
N.D.
N.D.
N.D.
N.D.
-0.5
carbon source
D-Lactate (C)
N.D.
N.D.
N.D.
N.D.
-0.5
stress
copper (II) chloride 2.5 mM
N.D.
N.D.
N.D.
N.D.
-0.5
nitrogen source
Ammonium chloride (N)
N.D.
N.D.
N.D.
N.D.
-0.5
stress
Doxycycline hyclate 0.0001 mg/ml
N.D.
N.D.
N.D.
N.D.
-0.5
carbon source
D,L-Lactate (C)
N.D.
N.D.
N.D.
N.D.
-0.4
carbon source
carbon source Sucrose 5mM
N.D.
N.D.
N.D.
N.D.
-0.4
nitrogen source
Cytosine (N)
N.D.
N.D.
N.D.
N.D.
-0.4
nitrogen source
L-Citrulline (N)
N.D.
N.D.
N.D.
N.D.
+0.5
stress
sodium fluoride 12.5 mM
N.D.
N.D.
N.D.
N.D.
+0.5
carbon source
Carnitine Hydrochloride (C)
N.D.
N.D.
N.D.
N.D.
+0.5
carbon source
carbon source Sucrose 0.25mM
N.D.
N.D.
N.D.
N.D.
+0.5
carbon source
D-Ribose (C)
N.D.
N.D.
N.D.
N.D.
+0.5
nitrogen source
Uridine (N)
N.D.
N.D.
N.D.
N.D.
+0.5
carbon source
Cytidine (C)
N.D.
N.D.
N.D.
N.D.
+0.5
anaerobic
N2E2wt with N1B4m denitrifying repA
N.D.
N.D.
N.D.
N.D.
+0.5
stress
Dimethyl Sulfoxide 5 vol%
N.D.
N.D.
N.D.
N.D.
+0.5
stress
D-Cycloserine 0.1 mg/ml
N.D.
N.D.
N.D.
N.D.
+0.5
carbon source
L-Glutamine (C)
N.D.
N.D.
N.D.
N.D.
+0.6
nitrogen source
L-Leucine (N)
N.D.
N.D.
N.D.
N.D.
+0.6
nitrogen source
Putrescine (N)
N.D.
N.D.
N.D.
N.D.
+0.6
temperature
LB 37C
N.D.
N.D.
N.D.
N.D.
+0.7
pH
Minimal media pH9
N.D.
N.D.
N.D.
N.D.
+0.7
stress
Fusidic 0.125 mg/ml
N.D.
N.D.
N.D.
N.D.
+0.8
nitrogen source
L-Methionine (N)
N.D.
N.D.
N.D.
N.D.
+0.8
stress
Aluminum chloride 2.5 mM
N.D.
N.D.
N.D.
N.D.
+0.9
anaerobic
N2E2wt with N1B4m denitrifying repA
N.D.
N.D.
N.D.
N.D.
+0.9
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