Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_1386 and Pf1N1B4_1387 are separated by 4 nucleotidesPf1N1B4_1387 and Pf1N1B4_1388 are separated by 192 nucleotidesPf1N1B4_1388 and Pf1N1B4_1389 are separated by 344 nucleotidesPf1N1B4_1389 and Pf1N1B4_1390 are separated by 68 nucleotides Pf1N1B4_1386 - Nicotinate phosphoribosyltransferase (EC 2.4.2.11), at 1,586,563 to 1,587,789 _1386 Pf1N1B4_1387 - NAD synthetase (EC 6.3.1.5), at 1,587,794 to 1,588,621 _1387 Pf1N1B4_1388 - Azurin, at 1,588,814 to 1,589,260 _1388 Pf1N1B4_1389 - Lysine decarboxylase family, at 1,589,605 to 1,590,192 _1389 Pf1N1B4_1390 - FIG00956548: hypothetical protein, at 1,590,261 to 1,590,449 _1390
Group Condition Pf1N1B4_1386 Pf1N1B4_1387 Pf1N1B4_1388 Pf1N1B4_1389 Pf1N1B4_1390
temperature LB 37C -0.8 N.D. -1.6 -0.7 -0.3
stress Sisomicin 0.001 mg/ml -0.2 N.D. -1.8 -0.9 -0.1
stress nitrate 100 mM -0.4 N.D. -1.0 -1.0 -0.5
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.9 N.D. +0.0 -1.2 -0.6
carbon source carbon source Sucrose 0.25mM -0.2 N.D. +0.8 -1.7 -1.2
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate B -0.3 N.D. -0.8 -1.0 -0.3
stress Choline chloride 200 mM -0.4 N.D. -0.5 -0.8 -0.4
carbon source D-Lactate (C) -0.7 N.D. -0.2 -0.9 -0.0
stress Nalidixic 0.004 mg/ml -0.7 N.D. -0.5 -0.2 -0.4
pH Minimal media pH9 -0.6 N.D. +0.3 -1.5 +0.1
carbon source carbon source Sucrose 1mM -1.1 N.D. +0.4 -0.5 -0.3
nitrogen source Thymine (N) +0.3 N.D. -0.6 -0.7 -0.5
nitrogen source nitrate (N) +0.3 N.D. -0.2 -1.1 -0.5
stress acetate 20 mM +0.3 N.D. -0.8 -0.7 -0.2
stress 1-ethyl-3-methylimidazolium chloride 80 mM +0.3 N.D. +0.1 -0.9 -0.6
nitrogen source Uridine (N) -0.3 N.D. +0.5 -0.9 -0.3
stress Gentamicin 0.001 mg/ml +0.4 N.D. -0.8 -0.1 -0.5
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1 -0.2 N.D. -1.2 +0.5 +0.2
denitrifying Nitrate reduction, replicate B t1 -0.4 N.D. -0.6 +0.6 -0.2
stress Chloride 300 mM +0.7 N.D. -0.5 -0.5 -0.3
stress Aluminum chloride 2.5 mM -0.9 N.D. +0.6 -0.3 +0.2
carbon source carbon source Sucrose 0.5mM +0.3 N.D. +0.4 +0.4 -0.9
stress Spectinomycin 0.025 mg/ml -0.9 N.D. +0.5 +0.4 +0.5
carbon source Tween 20 (C) +0.2 N.D. +0.9 -0.5 +0.3
carbon source octanoate (C) +0.6 N.D. +0.5 -0.7 +0.4
nitrogen source nitrogen source nitrite 0.1mM +0.8 N.D. -0.2 +0.7 -0.2
temperature LB 37C -0.5 N.D. +0.6 +1.1 -0.1
anaerobic Nitrate reduction -0.2 N.D. +1.5 -0.0 -0.2
stress Benzethonium chloride 0.0125 mM +0.7 N.D. +0.3 +0.7 -0.1
stress Chlorite 1 mM +0.6 N.D. +0.5 +0.6 +0.1
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