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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_1350 and Pf1N1B4_1351 are separated by 5 nucleotides
Pf1N1B4_1351 and Pf1N1B4_1352 overlap by 1 nucleotides
Pf1N1B4_1352 and Pf1N1B4_1353 are separated by 126 nucleotides
Pf1N1B4_1353 and Pf1N1B4_1354 are separated by 67 nucleotides
Pf1N1B4_1350 - GCN5-related N-acetyltransferase, at 1,546,404 to 1,546,937
_1350
Pf1N1B4_1351 - Tabtoxin resistance protein, at 1,546,943 to 1,547,476
_1351
Pf1N1B4_1352 - Urease beta subunit (EC 3.5.1.5), at 1,547,476 to 1,547,784
_1352
Pf1N1B4_1353 - Urease alpha subunit (EC 3.5.1.5), at 1,547,911 to 1,549,611
_1353
Pf1N1B4_1354 - hypothetical protein, at 1,549,679 to 1,552,135
_1354
Group
Condition
Pf1N1B4
_1350
Pf1N1B4
_1351
Pf1N1B4
_1352
Pf1N1B4
_1353
Pf1N1B4
_1354
anaerobic
N2E2wt with N1B4m denitrifying repB
-2.4
-0.5
N.D.
-0.5
-0.1
nitrogen source
nitrogen source nitrite 0.1mM
+0.2
-1.7
N.D.
+0.0
-0.1
nitrogen source
Adenine (N)
+0.6
-1.1
N.D.
-1.1
+0.1
anaerobic
Nitrate reduction
-0.4
-0.9
N.D.
-0.3
+0.1
nitrogen source
L-Lysine (N)
-1.0
-0.3
N.D.
+0.1
+0.1
carbon source
carbon source Sucrose 1mM
-0.1
+0.4
N.D.
-1.5
+0.2
carbon source
carbon source Sucrose 0.5mM
+0.6
-0.0
N.D.
-1.1
-0.4
denitrifying
Nitrate reduction, replicate C t3
-0.7
-0.4
N.D.
+0.3
-0.1
nitrogen source
L-Leucine (N)
-0.8
+0.2
N.D.
-0.5
+0.2
carbon source
L-Asparagine (C)
-0.5
+0.4
N.D.
-0.5
+0.0
stress
Choline chloride 200 mM
+0.7
-1.1
N.D.
+0.0
-0.0
stress
Nalidixic 0.004 mg/ml
+0.3
-0.8
N.D.
+0.2
-0.0
carbon source
L-Histidine (C)
-0.8
+0.3
N.D.
+0.2
+0.1
nitrogen source
nitrogen source nitrite 0.5mM
+0.7
-0.4
N.D.
-0.3
-0.1
nitrogen source
nitrogen source nitrite 0.5mM
+0.8
-0.0
N.D.
-0.7
-0.2
carbon source
carbon source Sucrose 1mM
+0.5
+0.2
N.D.
-0.6
-0.1
carbon source
Tween 20 (C)
-0.5
-0.2
N.D.
+0.8
-0.1
stress
Sisomicin 0.001 mg/ml
+0.5
-0.5
N.D.
+0.4
-0.1
carbon source
carbon source Sucrose 5mM
-0.6
+0.6
N.D.
+0.6
-0.3
carbon source
carbon source Sucrose 0.25mM
+1.4
+0.3
N.D.
-1.6
+0.1
stress
Spectinomycin 0.05 mg/ml
+0.3
-0.6
N.D.
+0.6
+0.1
stress
Cholin acetate 20 mM
+0.9
-0.1
N.D.
-0.5
+0.0
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
+0.8
-0.3
N.D.
+0.3
-0.2
nitrogen source
nitrogen source nitrate 20mM
+1.0
+0.4
N.D.
-0.5
-0.2
stress
Tetracycline 0.0004 mg/ml
+0.4
-0.3
N.D.
+0.6
+0.2
lb
LB
+0.5
-0.3
N.D.
+0.6
+0.1
denitrifying
Nitrate reduction, replicate A
+0.5
+0.8
N.D.
-0.0
-0.1
denitrifying
Nitrate reduction, replicate C
+0.7
+0.3
N.D.
+0.4
+0.0
stress
Doxycycline hyclate 0.0001 mg/ml
+0.8
-0.1
N.D.
+0.9
-0.0
stress
Benzethonium chloride 0.0125 mM
+0.9
+0.6
N.D.
+1.0
+0.3
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