Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_1348 and Pf1N1B4_1349 are separated by 158 nucleotidesPf1N1B4_1349 and Pf1N1B4_1350 are separated by 9 nucleotidesPf1N1B4_1350 and Pf1N1B4_1351 are separated by 5 nucleotidesPf1N1B4_1351 and Pf1N1B4_1352 overlap by 1 nucleotides Pf1N1B4_1348 - Urease accessory protein UreD, at 1,545,094 to 1,545,933 _1348 Pf1N1B4_1349 - Urease gamma subunit (EC 3.5.1.5), at 1,546,092 to 1,546,394 _1349 Pf1N1B4_1350 - GCN5-related N-acetyltransferase, at 1,546,404 to 1,546,937 _1350 Pf1N1B4_1351 - Tabtoxin resistance protein, at 1,546,943 to 1,547,476 _1351 Pf1N1B4_1352 - Urease beta subunit (EC 3.5.1.5), at 1,547,476 to 1,547,784 _1352
Group Condition Pf1N1B4_1348 Pf1N1B4_1349 Pf1N1B4_1350 Pf1N1B4_1351 Pf1N1B4_1352
anaerobic N2E2wt with N1B4m denitrifying repB +0.7 N.D. -2.4 -0.5 N.D.
nitrogen source Adenine (N) -1.5 N.D. +0.6 -1.1 N.D.
denitrifying Nitrate reduction, replicate C t3 -0.3 N.D. -0.7 -0.4 N.D.
nitrogen source nitrogen source nitrite 0.1mM +0.0 N.D. +0.2 -1.7 N.D.
pH Minimal media pH6 -0.5 N.D. -0.8 +0.2 N.D.
nitrogen source nitrogen source nitrite 1mM -0.7 N.D. +0.3 -0.6 N.D.
denitrifying Nitrate reduction, replicate B -0.9 N.D. +0.3 -0.3 N.D.
stress Spectinomycin 0.05 mg/ml -0.5 N.D. +0.3 -0.6 N.D.
carbon source carbon source Sucrose 1mM -1.6 N.D. +0.5 +0.2 N.D.
pH Minimal media pH9 -0.7 N.D. -0.4 +0.4 N.D.
carbon source L-Lactate (C) -1.0 N.D. +0.2 +0.1 N.D.
nitrogen source Uridine (N) -1.5 N.D. +0.7 +0.3 N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A -0.4 N.D. +0.5 -0.5 N.D.
carbon source carbon source Sucrose 5mM -0.4 N.D. -0.6 +0.6 N.D.
stress Nalidixic 0.004 mg/ml +0.2 N.D. +0.3 -0.8 N.D.
lb LB +0.7 N.D. -0.7 -0.2 N.D.
anaerobic N2E2wt with N1B4m denitrifying repA +0.7 N.D. -0.5 -0.4 N.D.
nitrogen source L-Leucine (N) +0.4 N.D. -0.8 +0.2 N.D.
carbon source D,L-Lactate (C) -0.7 N.D. +0.5 +0.2 N.D.
stress Choline chloride 200 mM +0.3 N.D. +0.7 -1.1 N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A -0.4 N.D. +0.8 -0.3 N.D.
stress Benzethonium chloride 0.0125 mM -1.1 N.D. +0.9 +0.6 N.D.
stress Cisplatin 0.006 mg/ml -0.5 N.D. +0.5 +0.3 N.D.
carbon source a-Ketoglutaric (C) +0.5 N.D. +0.5 -0.5 N.D.
carbon source carbon source Sucrose 0.25mM -1.0 N.D. +1.4 +0.3 N.D.
denitrifying Nitrate reduction, replicate A -0.4 N.D. +0.5 +0.8 N.D.
stress Fusidic 0.125 mg/ml +1.1 N.D. +0.4 -0.1 N.D.
carbon source carbon source Sucrose 0.25mM +0.8 N.D. +0.7 -0.1 N.D.
nitrogen source nitrogen source nitrate 20mM +0.1 N.D. +1.0 +0.4 N.D.
carbon source octanoate (C) +1.7 N.D. +0.3 -0.2 N.D.
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