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Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_1348 and Pf1N1B4_1349 are separated by 158 nucleotides
Pf1N1B4_1349 and Pf1N1B4_1350 are separated by 9 nucleotides
Pf1N1B4_1350 and Pf1N1B4_1351 are separated by 5 nucleotides
Pf1N1B4_1351 and Pf1N1B4_1352 overlap by 1 nucleotides
Pf1N1B4_1348 - Urease accessory protein UreD, at 1,545,094 to 1,545,933
_1348
Pf1N1B4_1349 - Urease gamma subunit (EC 3.5.1.5), at 1,546,092 to 1,546,394
_1349
Pf1N1B4_1350 - GCN5-related N-acetyltransferase, at 1,546,404 to 1,546,937
_1350
Pf1N1B4_1351 - Tabtoxin resistance protein, at 1,546,943 to 1,547,476
_1351
Pf1N1B4_1352 - Urease beta subunit (EC 3.5.1.5), at 1,547,476 to 1,547,784
_1352
Group
Condition
Pf1N1B4
_1348
Pf1N1B4
_1349
Pf1N1B4
_1350
Pf1N1B4
_1351
Pf1N1B4
_1352
anaerobic
N2E2wt with N1B4m denitrifying repB
+0.7
N.D.
-2.4
-0.5
N.D.
nitrogen source
Adenine (N)
-1.5
N.D.
+0.6
-1.1
N.D.
denitrifying
Nitrate reduction, replicate C t3
-0.3
N.D.
-0.7
-0.4
N.D.
nitrogen source
nitrogen source nitrite 0.1mM
+0.0
N.D.
+0.2
-1.7
N.D.
pH
Minimal media pH6
-0.5
N.D.
-0.8
+0.2
N.D.
nitrogen source
nitrogen source nitrite 1mM
-0.7
N.D.
+0.3
-0.6
N.D.
denitrifying
Nitrate reduction, replicate B
-0.9
N.D.
+0.3
-0.3
N.D.
stress
Spectinomycin 0.05 mg/ml
-0.5
N.D.
+0.3
-0.6
N.D.
carbon source
carbon source Sucrose 1mM
-1.6
N.D.
+0.5
+0.2
N.D.
pH
Minimal media pH9
-0.7
N.D.
-0.4
+0.4
N.D.
carbon source
L-Lactate (C)
-1.0
N.D.
+0.2
+0.1
N.D.
nitrogen source
Uridine (N)
-1.5
N.D.
+0.7
+0.3
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
-0.4
N.D.
+0.5
-0.5
N.D.
carbon source
carbon source Sucrose 5mM
-0.4
N.D.
-0.6
+0.6
N.D.
stress
Nalidixic 0.004 mg/ml
+0.2
N.D.
+0.3
-0.8
N.D.
lb
LB
+0.7
N.D.
-0.7
-0.2
N.D.
anaerobic
N2E2wt with N1B4m denitrifying repA
+0.7
N.D.
-0.5
-0.4
N.D.
nitrogen source
L-Leucine (N)
+0.4
N.D.
-0.8
+0.2
N.D.
carbon source
D,L-Lactate (C)
-0.7
N.D.
+0.5
+0.2
N.D.
stress
Choline chloride 200 mM
+0.3
N.D.
+0.7
-1.1
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
-0.4
N.D.
+0.8
-0.3
N.D.
stress
Benzethonium chloride 0.0125 mM
-1.1
N.D.
+0.9
+0.6
N.D.
stress
Cisplatin 0.006 mg/ml
-0.5
N.D.
+0.5
+0.3
N.D.
carbon source
a-Ketoglutaric (C)
+0.5
N.D.
+0.5
-0.5
N.D.
carbon source
carbon source Sucrose 0.25mM
-1.0
N.D.
+1.4
+0.3
N.D.
denitrifying
Nitrate reduction, replicate A
-0.4
N.D.
+0.5
+0.8
N.D.
stress
Fusidic 0.125 mg/ml
+1.1
N.D.
+0.4
-0.1
N.D.
carbon source
carbon source Sucrose 0.25mM
+0.8
N.D.
+0.7
-0.1
N.D.
nitrogen source
nitrogen source nitrate 20mM
+0.1
N.D.
+1.0
+0.4
N.D.
carbon source
octanoate (C)
+1.7
N.D.
+0.3
-0.2
N.D.
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