Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_1332 and Pf1N1B4_1333 overlap by 4 nucleotidesPf1N1B4_1333 and Pf1N1B4_1334 are separated by 272 nucleotidesPf1N1B4_1334 and Pf1N1B4_1335 are separated by 48 nucleotidesPf1N1B4_1335 and Pf1N1B4_1336 are separated by 66 nucleotides Pf1N1B4_1332 - Cobalt-precorrin-6 synthase, anaerobic, at 1,527,328 to 1,528,425 _1332 Pf1N1B4_1333 - Cobalt-precorrin-6x reductase (EC 1.3.1.54), at 1,528,422 to 1,529,156 _1333 Pf1N1B4_1334 - FIG00955840: hypothetical protein, at 1,529,429 to 1,529,827 _1334 Pf1N1B4_1335 - Copper metallochaperone, bacterial analog of Cox17 protein, at 1,529,876 to 1,530,355 _1335 Pf1N1B4_1336 - Putative multicopper oxidases, at 1,530,422 to 1,530,826 _1336
Group Condition Pf1N1B4_1332 Pf1N1B4_1333 Pf1N1B4_1334 Pf1N1B4_1335 Pf1N1B4_1336
temperature LB 20C -0.0 +0.1 -1.5 -1.6 -0.8
stress Chlorite 1 mM +0.1 -0.4 -0.2 -1.8 -1.4
nophagecontrol Time16-nophage +0.0 -0.0 -2.2 -0.5 -0.3
carbon source D-Ribose (C) -1.0 -0.2 -0.5 +0.4 -1.1
stress Cephalothin 2.5 mg/ml +0.4 +0.1 +0.1 -1.6 -1.4
anaerobic N2E2wt with N1B4m denitrifying repA -1.9 +0.2 -1.0 +0.1 +0.3
temperature LB 37C -2.9 +0.1 +1.6 +0.9 -1.7
carbon source Fumarate (C) -1.0 -0.3 -1.3 +0.2 +0.6
nophagecontrol Time16-nophage -0.2 +0.1 -1.3 -0.9 +0.7
carbon source D-Glucose (C) +0.1 -0.0 -2.5 +0.3 +0.7
survival 4C survival; 24 hrs +0.2 +0.1 -0.6 -1.7 +1.4
carbon source Putrescine (C) -1.4 -1.2 +0.9 +0.3 +0.7
carbon source L-Arginine (C) -0.5 -1.2 +0.8 +0.7 -0.1
carbon source L-Asparagine (C) +0.3 -1.0 +0.9 +0.3 +0.7
stress Spectinomycin 0.05 mg/ml -0.2 +0.3 +1.4 +1.0 -1.1
stress Fusidic 0.0625 mg/ml -0.5 -0.0 +1.6 +1.0 -0.1
carbon source carbon source Sucrose 0.25mM +0.9 +0.2 +1.5 +1.5 -2.1
carbon source carbon source Sucrose 1mM -1.1 -0.6 +1.1 +1.6 +1.2
stress methylglyoxal 0.008 vol% -0.2 +0.3 +1.4 +1.0 -0.4
stress nitrate 100 mM +0.5 +0.5 +1.2 +0.8 -0.5
nitrogen source Cytidine (N) -0.1 +0.5 +1.6 +1.1 -0.5
pH Minimal media pH8 +0.4 -0.3 +1.2 +0.7 +0.9
stress Choline chloride 200 mM +0.3 -0.2 +1.5 +1.3 +0.5
stress Sisomicin 0.001 mg/ml +0.2 +0.6 +1.5 +1.3 +0.3
carbon source carbon source Sucrose 0.5mM +0.9 +0.4 +1.3 +1.7 -0.1
stress Paraquat dichloride 0.016 mg/mL +1.2 -0.2 +1.2 +1.7 +0.7
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 -0.1 +0.4 +2.2 +0.4 +1.9
stress Doxycycline hyclate 0.0001 mg/ml -1.0 +0.3 +1.8 +1.2 +2.4
pH Minimal media pH9 -0.1 -0.3 +2.3 +2.3 +1.6
pH Minimal media pH9 +0.3 +0.1 +2.6 +2.3 +1.8
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