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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
Pf1N1B4_10 and Pf1N1B4_11 are separated by 280 nucleotides
Pf1N1B4_11 and Pf1N1B4_12 are separated by 67 nucleotides
Pf1N1B4_12 and Pf1N1B4_13 are separated by 93 nucleotides
Pf1N1B4_13 and Pf1N1B4_14 are separated by 152 nucleotides
Pf1N1B4_10 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 10,802 to 11,812
_10
Pf1N1B4_11 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 12,093 to 13,205
_11
Pf1N1B4_12 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 13,273 to 14,355
_12
Pf1N1B4_13 - SAM-dependent methyltransferase YafE (UbiE paralog), at 14,449 to 15,216
_13
Pf1N1B4_14 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 15,369 to 16,013
_14
Group
Condition
Pf1N1B4
_10
Pf1N1B4
_11
Pf1N1B4
_12
Pf1N1B4
_13
Pf1N1B4
_14
carbon source
carbon source Sucrose 0.25mM
+0.0
N.D.
-3.8
-4.2
N.D.
carbon source
carbon source Sucrose 0.25mM
-0.3
N.D.
-3.4
-2.6
N.D.
carbon source
carbon source Sucrose 0.5mM
-0.3
N.D.
-2.3
-2.8
N.D.
pH
Minimal media pH9
-1.6
N.D.
-3.0
-0.8
N.D.
pH
Minimal media pH8
-0.6
N.D.
-3.5
-0.8
N.D.
nitrogen source
nitrogen source nitrate 5mM
-0.1
N.D.
-3.5
-1.0
N.D.
nitrogen source
nitrogen source nitrate 5mM
-0.3
N.D.
-3.8
-0.4
N.D.
carbon source
L-Arabinose (C)
+0.1
N.D.
-3.4
-1.0
N.D.
nitrogen source
nitrogen source Ammonium chloride 20mM
-0.6
N.D.
-3.4
-0.0
N.D.
nitrogen source
L-Serine (N)
-0.0
N.D.
-3.2
-0.7
N.D.
carbon source
D-Lactate (C)
-0.1
N.D.
-3.0
-0.8
N.D.
carbon source
L-Arabinose (C)
+0.2
N.D.
-3.4
-0.7
N.D.
carbon source
D-Gluconic Acid (C)
-0.3
N.D.
-2.5
-1.1
N.D.
pH
Minimal media pH7
-0.1
N.D.
-3.0
-0.8
N.D.
carbon source
carbon source Sucrose 1mM
+0.9
N.D.
-3.2
-1.4
N.D.
nitrogen source
nitrogen source nitrate 20mM
+0.2
N.D.
-3.6
-0.3
N.D.
pH
Minimal media pH6
+0.1
N.D.
-3.2
-0.5
N.D.
carbon source
L-Lactate (C)
+0.1
N.D.
-3.0
-0.8
N.D.
nitrogen source
nitrogen source Ammonium chloride 20mM
+0.4
N.D.
-3.1
-0.8
N.D.
carbon source
a-Ketoglutaric (C)
+0.5
N.D.
-3.6
-0.4
N.D.
nitrogen source
L-Arginine (N)
+0.3
N.D.
-3.2
-0.4
N.D.
carbon source
a-Ketoglutaric (C)
+0.3
N.D.
-3.2
-0.3
N.D.
carbon source
octanoate (C)
-1.0
N.D.
-2.6
+0.5
N.D.
carbon source
D-Ribose (C)
+0.6
N.D.
-3.0
-0.4
N.D.
carbon source
carbon source Sucrose 1mM
+0.6
N.D.
-2.2
-1.0
N.D.
nitrogen source
nitrate (N)
+1.1
N.D.
-2.7
-1.0
N.D.
nitrogen source
Ammonium chloride (N)
+0.9
N.D.
-2.7
-0.8
N.D.
carbon source
m-Inositol (C)
-0.5
N.D.
-2.5
+0.9
N.D.
carbon source
Uridine (C)
-0.4
N.D.
-2.5
+0.9
N.D.
nitrogen source
D-Alanine (N)
+0.3
N.D.
-2.9
+0.6
N.D.
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