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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_114 and Pf1N1B4_115 are separated by 169 nucleotides
Pf1N1B4_115 and Pf1N1B4_116 are separated by 113 nucleotides
Pf1N1B4_116 and Pf1N1B4_117 are separated by 88 nucleotides
Pf1N1B4_117 and Pf1N1B4_118 are separated by 40 nucleotides
Pf1N1B4_114 - Diaminopimelate epimerase (EC 5.1.1.7), at 118,869 to 119,858
_114
Pf1N1B4_115 - Membrane protein mosC, at 120,028 to 121,179
_115
Pf1N1B4_116 - Iron(III) dicitrate transport protein FecA @ Iron siderophore receptor protein, at 121,293 to 123,725
_116
Pf1N1B4_117 - Iron siderophore sensor protein, at 123,814 to 124,788
_117
Pf1N1B4_118 - Sigma-70 factor FpvI (ECF subfamily), controling pyoverdin biosynthesis @ FIG006045: Sigma factor, ECF subfamily, at 124,829 to 125,329
_118
Group
Condition
Pf1N1B4
_114
Pf1N1B4
_115
Pf1N1B4
_116
Pf1N1B4
_117
Pf1N1B4
_118
carbon source
carbon source Sucrose 0.25mM
-0.6
-1.4
-0.2
-0.0
-0.7
temperature
LB 37C
+0.2
-0.7
-0.2
+0.2
-2.2
stress
nitrate 100 mM
-0.6
-1.7
-0.0
-0.4
+0.3
carbon source
carbon source Sucrose 0.5mM
-0.5
+0.8
-0.2
-0.2
-1.9
carbon source
carbon source Sucrose 5mM
-0.5
-1.2
+0.1
+0.4
-0.5
stress
benzoic 0.58 mg/ml
+0.8
-1.4
-0.2
-0.3
-0.6
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
-0.7
-1.4
-0.3
+0.3
+0.7
carbon source
carbon source Sucrose 1mM
+0.4
-2.1
-0.1
+0.5
+0.0
nitrogen source
Cytidine (N)
-1.5
-0.2
+0.1
+0.9
-0.5
pH
Minimal media pH9
-0.8
+0.6
-0.1
-0.3
-0.4
anaerobic
Nitrate reduction
+0.4
-1.2
+0.1
+0.2
-0.4
carbon source
octanoate (C)
-0.0
-1.7
-0.0
+0.5
+0.3
denitrifying
Nitrate reduction, replicate B t2
+0.5
-0.8
-0.3
+0.2
-0.3
nitrogen source
Thymine (N)
-1.1
-0.4
-0.1
+0.9
+0.2
anaerobic
N2E2wt with N1B4m denitrifying repA
-1.3
-0.2
-0.2
+0.3
+0.9
carbon source
carbon source Sucrose 20mM
+0.3
-1.2
-0.1
+0.1
+0.5
carbon source
D-Mannose (C)
+0.3
-0.8
+0.4
+0.7
-1.0
denitrifying
Nitrate reduction, replicate A t2
+1.3
-0.3
-0.2
-0.8
-0.3
stress
Rifampicin 0.004 mg/ml
+0.2
+0.7
+0.0
-0.3
-0.9
stress
Doxycycline hyclate 0.0001 mg/ml
+0.7
-1.4
-0.4
+0.4
+0.4
carbon source
L-Leucine (C)
+0.6
-0.7
+0.0
+0.5
-0.4
stress
Spectinomycin 0.025 mg/ml
+0.9
-0.8
-0.0
+0.3
-0.2
denitrifying
Nitrate reduction, replicate A t1
+1.2
+0.3
-0.2
-0.2
-0.6
lb
LB
-0.5
+0.3
+0.1
-0.3
+0.9
carbon source
carbon source Sucrose 1mM
-1.1
+0.4
+0.0
+0.7
+0.8
stress
copper (II) chloride 2.5 mM
+0.0
+0.7
+0.1
-0.7
+0.7
denitrifying
Nitrate reduction, replicate C t3
+1.5
-0.5
-0.1
+0.2
-0.1
phagefrs
Time6-FRS
+0.7
+0.3
-0.1
+0.6
+0.6
anaerobic
Nitrate reduction
+0.7
+1.8
+0.2
+0.3
-0.1
carbon source
carbon source Sucrose 5mM
+1.1
+0.9
+0.1
+0.6
+0.9
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