Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_1131 and Pf1N1B4_1132 are separated by 20 nucleotidesPf1N1B4_1132 and Pf1N1B4_1133 are separated by 12 nucleotidesPf1N1B4_1133 and Pf1N1B4_1134 overlap by 4 nucleotidesPf1N1B4_1134 and Pf1N1B4_1135 are separated by 179 nucleotides Pf1N1B4_1131 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,269,525 to 1,270,241 _1131 Pf1N1B4_1132 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) / Osmotic adaptation, at 1,270,262 to 1,271,155 _1132 Pf1N1B4_1133 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,271,168 to 1,271,821 _1133 Pf1N1B4_1134 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 1,271,818 to 1,272,975 _1134 Pf1N1B4_1135 - FIG00960055: hypothetical protein, at 1,273,155 to 1,273,307 _1135
Group Condition Pf1N1B4_1131 Pf1N1B4_1132 Pf1N1B4_1133 Pf1N1B4_1134 Pf1N1B4_1135
stress Chloride 300 mM -0.9 -0.6 -1.2 -0.7 +0.6
carbon source carbon source Sucrose 0.5mM -0.7 -0.9 +0.1 -0.4 -0.4
carbon source carbon source Sucrose 5mM -0.7 -0.3 +0.1 -0.4 -0.8
anaerobic N2E2wt with N1B4m denitrifying repB +0.0 +0.2 -0.3 +0.1 -2.2
denitrifying Nitrate reduction, replicate C t3 -0.3 -0.4 -0.1 -0.1 -1.0
carbon source carbon source Sucrose 1mM -0.1 -0.5 +0.8 -0.4 -1.7
temperature LB 37C +0.3 +0.0 -0.4 +0.1 -1.7
stress 1-ethyl-3-methylimidazolium chloride 80 mM -0.5 -0.7 -0.4 -0.7 +0.8
nitrogen source nitrogen source nitrate 20mM +0.2 -0.1 +0.1 -0.7 -1.0
carbon source carbon source Sucrose 0.25mM +0.0 -1.3 +0.6 -0.6 -0.2
nitrogen source nitrogen source nitrite 0.1mM +0.3 -0.7 +0.1 +0.0 -1.1
stress nitrate 100 mM -0.1 -0.6 -0.5 -0.3 +0.3
carbon source m-Inositol (C) +0.2 -0.5 +0.2 +0.3 -1.0
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 +0.7 -0.3 +0.5 +0.2 -1.9
stress Chloride 200 mM -0.3 -0.4 -0.6 -0.5 +1.1
carbon source Uridine (C) +0.2 -0.2 +0.2 +0.4 -1.0
nitrogen source Cytidine (N) +0.4 -0.1 +0.4 -0.0 -0.9
stress Dimethyl Sulfoxide 5 vol% +0.1 -0.2 +0.4 +0.4 -0.7
carbon source L-Alanine (C) -0.1 -0.3 +0.7 +0.3 -0.6
carbon source Carnitine Hydrochloride (C) +0.4 -0.5 +0.4 +0.3 -0.5
stress Fusidic 0.125 mg/ml +0.3 +0.3 +1.0 +0.5 -2.0
denitrifying Nitrate reduction, replicate C +0.3 +0.1 +0.3 +0.4 -0.6
nitrogen source nitrogen source Ammonium chloride 20mM +0.6 +0.6 +0.3 -0.6 -0.1
carbon source carbon source Sucrose 0.25mM -0.1 +0.8 +0.9 -0.3 -0.2
stress Carbenicillin 2 mg/ml -0.1 +0.5 -0.2 +0.1 +1.0
stress Cisplatin 0.006 mg/ml +0.5 +0.3 -0.2 -0.2 +1.1
stress Fusidic 0.0625 mg/ml +0.3 +0.1 +0.4 +0.4 +0.7
stress methylglyoxal 0.008 vol% +0.3 +0.3 +0.4 +0.0 +0.9
pH Minimal media pH9 +0.5 +0.3 +0.4 +0.1 +1.3
stress Paraquat dichloride 0.016 mg/mL +0.6 +0.5 +0.8 +0.4 +0.9
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