Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_1129 and Pf1N1B4_1130 overlap by 19 nucleotidesPf1N1B4_1130 and Pf1N1B4_1131 are separated by 502 nucleotidesPf1N1B4_1131 and Pf1N1B4_1132 are separated by 20 nucleotidesPf1N1B4_1132 and Pf1N1B4_1133 are separated by 12 nucleotides Pf1N1B4_1129 - Peptide chain release factor 3, at 1,267,440 to 1,267,691 _1129 Pf1N1B4_1130 - Peptide chain release factor 3, at 1,267,673 to 1,269,022 _1130 Pf1N1B4_1131 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,269,525 to 1,270,241 _1131 Pf1N1B4_1132 - L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1) / Osmotic adaptation, at 1,270,262 to 1,271,155 _1132 Pf1N1B4_1133 - L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1), at 1,271,168 to 1,271,821 _1133
Group Condition Pf1N1B4_1129 Pf1N1B4_1130 Pf1N1B4_1131 Pf1N1B4_1132 Pf1N1B4_1133
stress Chloride 300 mM N.D. N.D. -0.9 -0.6 -1.2
stress 1-ethyl-3-methylimidazolium chloride 80 mM N.D. N.D. -0.5 -0.7 -0.4
nitrogen source L-Serine (N) N.D. N.D. -0.6 -0.3 -0.6
carbon source carbon source Sucrose 0.5mM N.D. N.D. -0.7 -0.9 +0.1
stress Chloride 200 mM N.D. N.D. -0.3 -0.4 -0.6
stress nitrate 100 mM N.D. N.D. -0.1 -0.6 -0.5
anaerobic N2E2wt with N1B4m denitrifying repA N.D. N.D. -0.5 -0.1 -0.5
nophagecontrol TIme6-nophage N.D. N.D. -0.3 -0.4 -0.4
carbon source carbon source Sucrose 5mM N.D. N.D. -0.7 -0.3 +0.1
carbon source carbon source Sucrose 0.25mM N.D. N.D. +0.0 -1.3 +0.6
stress sodium fluoride 12.5 mM N.D. N.D. -0.3 +0.2 -0.5
stress D-Cycloserine 0.1 mg/ml N.D. N.D. -0.7 -0.2 +0.5
nitrogen source nitrogen source nitrite 0.1mM N.D. N.D. +0.3 -0.7 +0.1
carbon source L-Leucine (C) N.D. N.D. +0.0 -0.6 +0.4
carbon source L-Glutamine (C) N.D. N.D. -0.1 -0.4 +0.4
carbon source m-Inositol (C) N.D. N.D. +0.2 -0.5 +0.2
nitrogen source L-Methionine (N) N.D. N.D. +0.2 -0.5 +0.2
carbon source carbon source Sucrose 1mM N.D. N.D. -0.1 -0.5 +0.8
carbon source D-Trehalose (C) N.D. N.D. +0.2 -0.5 +0.6
carbon source Carnitine Hydrochloride (C) N.D. N.D. +0.4 -0.5 +0.4
carbon source L-Alanine (C) N.D. N.D. -0.1 -0.3 +0.7
carbon source L-Valine (C) N.D. N.D. +0.2 -0.3 +0.5
stress Cisplatin 0.006 mg/ml N.D. N.D. +0.5 +0.3 -0.2
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 N.D. N.D. +0.7 -0.3 +0.5
stress methylglyoxal 0.008 vol% N.D. N.D. +0.3 +0.3 +0.4
pH Minimal media pH9 N.D. N.D. +0.5 +0.3 +0.4
nitrogen source nitrogen source Ammonium chloride 20mM N.D. N.D. +0.6 +0.6 +0.3
carbon source carbon source Sucrose 0.25mM N.D. N.D. -0.1 +0.8 +0.9
stress Fusidic 0.125 mg/ml N.D. N.D. +0.3 +0.3 +1.0
stress Paraquat dichloride 0.016 mg/mL N.D. N.D. +0.6 +0.5 +0.8
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