Fitness for 5 genes in Pseudomonas fluorescens FW300-N1B4

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500 ntPf1N1B4_1103 and Pf1N1B4_1104 are separated by 50 nucleotidesPf1N1B4_1104 and Pf1N1B4_1105 overlap by 4 nucleotidesPf1N1B4_1105 and Pf1N1B4_1106 are separated by 155 nucleotidesPf1N1B4_1106 and Pf1N1B4_1107 are separated by 167 nucleotides Pf1N1B4_1103 - Rare lipoprotein A, at 1,236,771 to 1,237,145 _1103 Pf1N1B4_1104 - K+-dependent Na+/Ca+ exchanger related-protein, at 1,237,196 to 1,238,257 _1104 Pf1N1B4_1105 - 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), at 1,238,254 to 1,238,634 _1105 Pf1N1B4_1106 - membrane protein, putative, at 1,238,790 to 1,239,731 _1106 Pf1N1B4_1107 - D-galactarate dehydratase (EC 4.2.1.42), at 1,239,899 to 1,241,452 _1107
Group Condition Pf1N1B4_1103 Pf1N1B4_1104 Pf1N1B4_1105 Pf1N1B4_1106 Pf1N1B4_1107
nitrogen source nitrogen source nitrate 20mM +0.1 -0.5 -2.8 -0.2 -0.1
stress Benzethonium chloride 0.0125 mM -0.1 -0.5 -1.8 +0.0 -0.3
denitrifying Nitrate reduction, replicate C -0.3 +0.3 -2.0 -0.1 +0.0
nitrogen source nitrogen source nitrite 1mM +0.2 -0.1 -2.1 +0.2 -0.3
pH Minimal media pH9 -0.5 +0.2 -0.5 -0.6 -0.7
stress Spectinomycin 0.05 mg/ml -0.1 +0.3 -1.5 -0.1 -0.7
carbon source D-Galacturonic Acid (C) -0.4 +0.2 -0.4 +0.3 -1.7
anaerobic Nitrate reduction -0.9 -0.5 +0.0 +0.0 -0.6
carbon source L-Histidine (C) -0.1 +0.1 -1.4 -0.0 -0.4
anaerobic N2E2wt with N1B4m denitrifying repB -0.1 +0.2 -1.5 +0.0 -0.4
stress copper (II) chloride 2.5 mM -0.3 +0.2 -1.6 -0.3 +0.2
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A -0.1 +0.1 -0.5 +0.0 -1.1
stress Cephalothin 2.5 mg/ml -0.4 +0.3 -1.2 +0.1 -0.3
nitrogen source nitrogen source nitrite 0.1mM -0.5 +0.0 -0.6 +0.4 -0.6
stress Cisplatin 0.006 mg/ml -0.2 +0.4 -1.3 -0.3 +0.1
carbon source L-Asparagine (C) -0.5 -0.0 -1.3 +0.4 +0.2
stress benzoic 0.58 mg/ml +0.3 +0.0 -2.0 +0.4 +0.2
temperature LB 37C +0.2 +0.7 -0.8 -0.5 -0.5
stress Bacitracin 2 mg/ml +0.3 -0.3 -1.3 +0.1 +0.3
nitrogen source Carnitine Hydrochloride (N) -0.6 +0.4 -0.4 +0.3 -0.3
stress methylglyoxal 0.008 vol% -0.9 +0.3 +0.7 +0.0 -0.4
stress Fusidic 0.125 mg/ml -0.6 +0.4 +0.2 +0.3 -0.6
stress nitrate 100 mM -0.1 +0.2 +0.6 -0.1 -0.9
carbon source carbon source Sucrose 0.5mM +0.3 -0.6 +0.1 -0.4 +0.5
carbon source pyruvate (C) -0.2 +0.0 +1.0 -0.1 -0.8
carbon source carbon source Sucrose 1mM +0.3 -0.3 -0.6 +0.1 +0.7
stress Chloride 300 mM -0.6 +0.4 +0.9 +0.2 -0.6
denitrifying Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1 +0.5 -0.0 +0.8 +0.4 -1.2
carbon source D-Glucose (C) +0.4 -0.3 +0.9 +0.2 -0.5
carbon source carbon source Sucrose 5mM +0.3 +0.3 +1.0 -0.1 -0.8
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