Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS25255 and PFLU_RS25260 are separated by 26 nucleotidesPFLU_RS25260 and PFLU_RS31800 are separated by 79 nucleotidesPFLU_RS31800 and PFLU_RS25265 are separated by 162 nucleotidesPFLU_RS25265 and PFLU_RS25270 are separated by 173 nucleotides PFLU_RS25255: PFLU_RS25255 - DUF1329 domain-containing protein, at 5,645,161 to 5,646,519 _RS25255 PFLU_RS25260: PFLU_RS25260 - DUF1302 domain-containing protein, at 5,646,546 to 5,648,336 _RS25260 PFLU_RS31800: PFLU_RS31800 - hypothetical protein, at 5,648,416 to 5,648,658 _RS31800 PFLU_RS25265: PFLU_RS25265 - SDR family oxidoreductase, at 5,648,821 to 5,649,732 _RS25265 PFLU_RS25270: PFLU_RS25270 - alpha/beta fold hydrolase, at 5,649,906 to 5,650,811 _RS25270
Group Condition PFLU_RS25255 PFLU_RS25260 PFLU_RS31800 PFLU_RS25265 PFLU_RS25270
carbon source 2-Keto-D-gluconic hemicalcium salt (C) +0.1 +0.0 -6.0 -0.2 -0.1
temperature shift Temperature shift 10_to_25; with MOPS -0.0 +0.0 -5.4 -0.0 +0.1
carbon and nitrogen source NAG carbon and (N) -0.1 +0.1 -5.1 -0.1 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.1 +0.2 -6.0 +0.2 +0.4
no stress control Glycine betaine (C)(N); with MOPS -0.0 -0.0 -4.5 -0.0 -0.2
carbon source p-Coumaric (C) 2.5 mM +0.0 +0.1 -5.1 -0.1 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.1 +0.1 -4.4 -0.2 -0.1
pH Growth at pH9 and (C) Trisodium citrate -0.0 -0.1 -4.5 -0.0 +0.0
stress Betaine (C)(N); with MOPS; with chloride -0.1 +0.3 -5.0 +0.3 -0.2
stress Glucose (C) and Glycine betaine (N); with MOPS; with chloride +0.0 -0.1 -4.3 +0.1 -0.2
carbon source Quinic Acid (C) +0.1 +0.0 -4.2 +0.0 -0.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.1 +0.1 -4.6 +0.4 +0.1
stress Betaine (C)(N); with TAPS; with chloride -0.0 -0.1 -4.1 -0.2 +0.5
pH Growth at pH7 and (C) D-Glucose -0.1 +0.1 -3.9 +0.0 +0.2
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS -0.0 +0.0 -3.6 +0.1 -0.1
carbon source p-Coumaric (C) 5 mM +0.1 +0.1 -3.9 -0.1 +0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.1 -0.0 -3.3 -0.0 +0.1
carbon source Shikimic Acid (C) -0.1 +0.2 -2.9 +0.1 -0.3
pH Growth at pH8 and (C) Trisodium citrate -0.1 -0.3 -2.7 -0.2 +0.3
carbon source L-Carnitine (C) -0.1 +0.1 -2.3 -0.2 -0.6
carbon source 2-Keto-D-gluconic hemicalcium salt (C) -0.0 +0.2 -3.1 -0.1 +0.0
carbon source Trisodium citrate (C) -0.0 +0.1 -2.9 +0.2 -0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.2 +0.0 -2.2 +0.1 -0.8
carbon source Phloretic Acid 2 mM (C) +0.2 +0.4 -3.5 +0.4 -0.1
stress Betaine (C)(N); with TAPS; with chloride +0.2 +0.1 -3.0 -0.0 +0.3
carbon source Inosine 10 mM (C) +0.0 +0.1 -3.1 +0.3 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.0 +0.3 -3.0 +0.3 +0.1
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS +0.0 -0.0 -2.9 -0.1 +0.8
carbon source 2-Deoxy-D-Ribose 10 mM (C) -0.2 -0.1 +2.3 +0.1 -0.7
carbon source L-Valine 10 mM (C) -0.1 +0.1 +3.2 -0.3 +0.1
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