Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS19725 and PFLU_RS19730 are separated by 195 nucleotidesPFLU_RS19730 and PFLU_RS31700 are separated by 172 nucleotidesPFLU_RS31700 and PFLU_RS19735 are separated by 534 nucleotidesPFLU_RS19735 and PFLU_RS19740 are separated by 20 nucleotides PFLU_RS19725: PFLU_RS19725 - 4-aminobutyrate--2-oxoglutarate transaminase, at 4,462,242 to 4,463,534 _RS19725 PFLU_RS19730: PFLU_RS19730 - glyoxylate/hydroxypyruvate reductase A, at 4,463,730 to 4,464,656 _RS19730 PFLU_RS31700: PFLU_RS31700 - helix-turn-helix domain-containing protein, at 4,464,829 to 4,464,924 _RS31700 PFLU_RS19735: PFLU_RS19735 - tartrate dehydrogenase, at 4,465,459 to 4,466,544 _RS19735 PFLU_RS19740: PFLU_RS19740 - NAD-dependent succinate-semialdehyde dehydrogenase, at 4,466,565 to 4,468,025 _RS19740
Group Condition PFLU_RS19725 PFLU_RS19730 PFLU_RS31700 PFLU_RS19735 PFLU_RS19740
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.2 -3.4 N.D. +0.3 -0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.0 -3.0 N.D. -0.4 +0.1
stress Betaine (C)(N); with TAPS; with chloride -0.1 -2.5 N.D. -0.3 -0.3
stress Betaine (C)(N); with MOPS; with chloride +0.1 -2.6 N.D. +0.7 -1.3
carbon source L-Valine (C) -0.1 -2.4 N.D. +0.3 -0.7
carbon source p-Coumaric (C) -0.3 -1.6 N.D. -0.3 -0.5
carbon source L-Valine 10 mM (C) -0.6 -0.8 N.D. -0.2 -0.9
carbon source D-Mannitol (C) -0.3 -1.8 N.D. -0.4 -0.0
carbon source L-Valine (C) +0.0 -1.4 N.D. -0.4 -0.7
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.2 -1.4 N.D. -0.2 -1.0
nitrogen source NAG (N); with MOPS -0.5 -1.1 N.D. -0.5 -0.3
carbon source D-Glucuronic (C) -0.0 -2.2 N.D. +0.2 -0.3
no stress control Glycine betaine (C)(N); with TAPS +0.1 -1.9 N.D. +0.1 -0.4
carbon source p-Coumaric (C) 2.5 mM +0.4 -1.6 N.D. +0.1 -0.9
carbon and nitrogen source NAG carbon and (N) +0.1 -1.3 N.D. -0.4 -0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.8 +0.2 N.D. -0.4 -1.0
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.2 -1.8 N.D. -0.1 +0.2
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.2 -1.2 N.D. -0.2 -0.7
carbon and nitrogen source NAG carbon and (N) +0.4 -1.0 N.D. -0.4 -0.9
stress Betaine (C)(N); with TAPS; with chloride -0.1 -1.5 N.D. -0.5 +0.3
nitrogen source Urea (N); with MOPS +0.4 -2.0 N.D. -0.0 -0.1
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.1 -1.3 N.D. -0.5 +0.2
carbon source p-Coumaric 2 mM (C) +0.9 -2.1 N.D. -0.2 +0.0
carbon source L-Carnitine (C) -0.2 +0.8 N.D. +0.0 -1.8
carbon source 4-Guanidinobutyric (C) +0.4 -1.3 N.D. +0.2 -0.4
carbon source 2-Keto-D-gluconic hemicalcium salt (C) +0.2 -1.6 N.D. +0.1 +0.4
carbon source L-Ornithine 10 mM (C) -0.0 -1.7 N.D. +0.4 +0.5
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS +0.4 -1.0 N.D. -0.2 +0.7
stress Betaine (C)(N); with MOPS; with chloride +0.1 +0.6 N.D. +0.6 -1.0
pH Growth at pH9 and (C) Trisodium citrate +0.8 -1.1 N.D. +0.2 +0.5
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