Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS19475 and PFLU_RS19480 overlap by 4 nucleotidesPFLU_RS19480 and PFLU_RS31695 are separated by 741 nucleotidesPFLU_RS31695 and PFLU_RS19485 are separated by 263 nucleotidesPFLU_RS19485 and PFLU_RS19490 are separated by 58 nucleotides PFLU_RS19475: PFLU_RS19475 - Zn-dependent hydrolase, at 4,402,813 to 4,404,081 _RS19475 PFLU_RS19480: PFLU_RS19480 - amidase, at 4,404,078 to 4,405,484 _RS19480 PFLU_RS31695: PFLU_RS31695 - helix-turn-helix domain-containing protein, at 4,406,226 to 4,406,321 _RS31695 PFLU_RS19485: PFLU_RS19485 - LysR family transcriptional regulator, at 4,406,585 to 4,407,448 _RS19485 PFLU_RS19490: PFLU_RS19490 - LacI family transcriptional regulator, at 4,407,507 to 4,408,511 _RS19490
Group Condition PFLU_RS19475 PFLU_RS19480 PFLU_RS31695 PFLU_RS19485 PFLU_RS19490
pH Growth at pH9 and (C) Trisodium citrate -3.3 -0.6 N.D. -0.4 -0.5
carbon source L-Ornithine 10 mM (C) -1.2 -0.0 N.D. -0.2 -1.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -2.1 -0.2 N.D. -0.5 +0.3
carbon source L-Ornithine (C) -0.8 +0.2 N.D. -0.3 -1.6
carbon source L-Ornithine (C) -0.5 -0.1 N.D. -0.2 -1.7
carbon source L-Valine (C) -0.9 -0.4 N.D. +0.2 -1.4
no stress control Glycine betaine (C)(N); with MOPS -1.1 -0.0 N.D. -0.2 -1.0
carbon source NAG (C) -0.3 -0.6 N.D. -0.3 -1.1
carbon source D-Xylose 10 mM (C) -0.1 +0.4 N.D. -0.0 -2.4
carbon source caffeic 2 mM (C) -2.6 +0.7 N.D. -0.2 +0.0
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS -1.0 +0.1 N.D. +0.2 -1.2
stress Betaine (C)(N); with MOPS; with chloride -1.0 +0.4 N.D. -0.1 -0.9
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS -0.8 +0.5 N.D. -0.4 -0.6
temperature Growth at 25C -1.9 +0.4 N.D. +0.2 -0.0
carbon source D-Xylose (C) +0.2 +0.3 N.D. -0.0 -1.8
carbon source D-Xylose (C) +0.2 +0.3 N.D. +0.0 -1.8
carbon source propionate (C) -1.2 +0.5 N.D. -0.6 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -1.5 +0.0 N.D. +0.3 +0.4
carbon source D-Glucosamine Hydrochloride 10 mM (C) -1.2 -0.4 N.D. +0.1 +0.8
carbon source D-Xylose (C) -0.3 +0.8 N.D. +0.1 -1.1
carbon source NAG (C) +1.1 -0.2 N.D. -0.0 -1.2
temperature Growth at 25C +1.0 -0.2 N.D. -0.4 -0.7
stress Betaine (C)(N); with TAPS; with chloride +1.0 -0.2 N.D. +0.5 -0.8
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +1.1 +0.7 N.D. +0.6 -1.3
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +1.0 +0.4 N.D. +0.5 -0.5
no stress control Glycine betaine (C)(N); with TAPS +1.2 +0.5 N.D. +0.3 -0.5
carbon source L-Valine 10 mM (C) -0.0 +0.4 N.D. -0.4 +1.7
carbon source L-Valine 10 mM (C) -0.5 +0.6 N.D. +0.0 +2.6
carbon source Betaine 10 mM (C) +0.2 +0.1 N.D. +0.2 +2.3
carbon source D-Xylose 10 mM (C) -0.2 -0.3 N.D. +0.1 +5.8
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