Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS27580 and PFLU_RS27585 overlap by 8 nucleotidesPFLU_RS27585 and PFLU_RS31180 are separated by 5 nucleotidesPFLU_RS31180 and PFLU_RS27595 are separated by 185 nucleotidesPFLU_RS27595 and PFLU_RS27600 are separated by 209 nucleotides PFLU_RS27580: PFLU_RS27580 - pyrroloquinoline quinone biosynthesis peptide chaperone PqqD, at 6,137,629 to 6,137,904 _RS27580 PFLU_RS27585: PFLU_RS27585 - pyrroloquinoline quinone biosynthesis protein PqqE, at 6,137,897 to 6,139,027 _RS27585 PFLU_RS31180: PFLU_RS31180 - YqaE/Pmp3 family membrane protein, at 6,139,033 to 6,139,191 _RS31180 PFLU_RS27595: PFLU_RS27595 - aminotransferase class III-fold pyridoxal phosphate-dependent enzyme, at 6,139,377 to 6,140,645 _RS27595 PFLU_RS27600: PFLU_RS27600 - LysR family transcriptional regulator, at 6,140,855 to 6,141,754 _RS27600
Group Condition PFLU_RS27580 PFLU_RS27585 PFLU_RS31180 PFLU_RS27595 PFLU_RS27600
carbon source acetate 30 mM (C) -0.5 -0.2 -0.1 -1.9 -2.2
carbon source acetate (C) -0.9 -0.3 +0.3 -1.5 -1.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -2.5 -0.0 +0.6 -0.8 -0.9
carbon source p-Coumaric (C) 5 mM -2.2 +0.3 +0.3 -0.9 -0.9
no stress control L-Glutamine (C)(N); with MOPS -2.1 +0.1 -0.8 -0.5 +0.1
stress Betaine (C)(N); with MOPS; with chloride -3.1 -0.0 +0.1 -0.1 +0.0
carbon source acetate (C) -0.3 +0.0 +0.1 -1.3 -1.3
carbon source L-Valine 10 mM (C) -0.8 -0.0 -1.5 -0.1 -0.1
carbon source acetate 30 mM (C) -0.5 +0.1 +0.2 -1.0 -1.2
carbon source acetate 30 mM (C) +0.5 +0.4 +0.2 -1.7 -1.8
nitrogen source nitrate (N); with MOPS -2.5 -0.6 +0.3 +0.1 +0.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -0.2 -0.0 +0.3 -1.0 -1.3
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -1.1 +0.3 -0.3 -0.2 -0.7
carbon source acetate (C) +0.3 -0.2 -0.2 -0.9 -1.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -2.0 +0.4 +0.5 -0.3 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.8 +0.4 +0.5 -0.2 +0.1
carbon source p-Coumaric (C) 5 mM +0.4 +0.3 +0.3 -1.0 -0.7
pH Growth at pH7 and (C) D-Glucose -1.3 -0.3 +0.5 +0.4 +0.2
pH Growth at pH8 and (C) D-Glucose -1.1 -0.3 +0.5 +0.2 +0.4
carbon source p-Coumaric (C) 5 mM +0.8 +0.1 +0.3 -0.5 -0.8
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.7 +0.3 +0.3 -0.5 -0.9
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +1.0 -0.1 +0.9 -0.8 -0.9
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.9 -0.1 +0.5 -0.3 -0.8
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +1.1 +0.2 +0.5 -0.8 -0.6
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +1.2 +0.2 +0.3 -0.2 -0.8
carbon source L-Valine 10 mM (C) +1.3 -0.2 +0.7 -0.3 +0.0
carbon source propionate (C) -0.3 +0.2 -0.3 +1.0 +1.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +1.2 +0.2 +0.5 -0.3 +0.4
carbon source propionate (C) -0.4 +0.1 -0.1 +1.5 +1.3
carbon source propionate (C) +0.5 +0.2 +0.4 +1.6 +1.5
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