Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS11015 and PFLU_RS31540 overlap by 36 nucleotidesPFLU_RS31540 and PFLU_RS30670 are separated by 507 nucleotidesPFLU_RS30670 and PFLU_RS30675 are separated by 288 nucleotidesPFLU_RS30675 and PFLU_RS11020 are separated by 17 nucleotides PFLU_RS11015: PFLU_RS11015 - UTRA domain-containing protein, at 2,430,420 to 2,431,115 _RS11015 PFLU_RS31540: PFLU_RS31540 - ABC transporter ATP-binding protein, at 2,431,080 to 2,431,169 _RS31540 PFLU_RS30670: PFLU_RS30670 - DUF4102 domain-containing protein, at 2,431,677 to 2,432,003 _RS30670 PFLU_RS30675: PFLU_RS30675 - integrase, at 2,432,292 to 2,432,771 _RS30675 PFLU_RS11020: PFLU_RS11020 - OmpA family protein, at 2,432,789 to 2,433,757 _RS11020
Group Condition PFLU_RS11015 PFLU_RS31540 PFLU_RS30670 PFLU_RS30675 PFLU_RS11020
carbon source 4-Aminobutyric (C) -2.3 N.D. -0.8 -0.6 -0.0
nitrogen source NAG (N); with MOPS -1.2 N.D. -1.2 -0.2 -0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.1 N.D. -2.2 +0.0 -0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.1 N.D. -1.1 -0.3 -1.1
carbon source 4-Aminobutyric (C) -1.1 N.D. -0.6 -0.5 +0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.5 N.D. -1.4 +0.0 -0.2
carbon source Shikimic Acid (C) -1.2 N.D. -0.9 +0.0 +0.2
stress Betaine (C)(N); with TAPS; with chloride -1.9 N.D. -0.3 -0.2 +0.5
carbon source L-Lysine (C) +0.0 N.D. -0.9 -1.7 +0.6
carbon source D,L-Malic Acid (C) +0.2 N.D. -1.7 -0.3 -0.0
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -1.4 N.D. +0.3 -0.5 -0.2
carbon source Choline chloride 10 mM (C) -0.5 N.D. -1.0 -0.4 +0.2
no stress control Glucose (C) and L-Glutamine (N); with MOPS -0.4 N.D. -1.3 +0.3 -0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.5 N.D. -1.3 +0.2 +0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.8 N.D. -0.9 +0.3 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.0 N.D. -1.7 +0.4 +0.2
pH Growth at pH9 and (C) Trisodium citrate +0.2 N.D. -0.9 +0.5 -0.4
carbon source 2-Deoxy-D-Ribose 10 mM (C) +0.3 N.D. -1.1 +0.3 +0.2
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS -1.0 N.D. +0.6 -0.3 +0.4
stress Betaine (C)(N); with TAPS; with chloride +0.3 N.D. +0.8 -0.7 -0.4
carbon source NAG (C) -0.9 N.D. +1.0 -0.1 +0.0
carbon source D-Glucosamine Hydrochloride 10 mM (C) -0.9 N.D. +1.8 -0.2 -0.5
no stress control Glycine betaine (C)(N); with MOPS +0.9 N.D. -0.9 +0.5 -0.1
stress Betaine (C)(N); with MOPS; with chloride +0.8 N.D. +0.5 -0.4 -0.4
stress L-Glutamine (C)(N); with MOPS; with chloride -0.6 N.D. +0.9 +0.4 -0.2
carbon source NAG (C) -0.7 N.D. +0.9 -0.0 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.3 N.D. +0.7 -0.7 +0.2
carbon source L-Carnitine (C) -0.1 N.D. +1.4 -0.3 -0.4
carbon source L-Valine (C) +0.3 N.D. -0.5 +0.7 +0.4
stress Betaine (C)(N); with MOPS; with chloride +1.3 N.D. -0.1 +0.1 +0.5
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