Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS31520 and PFLU_RS10265 are separated by 155 nucleotidesPFLU_RS10265 and PFLU_RS30630 are separated by 261 nucleotidesPFLU_RS30630 and PFLU_RS10270 overlap by 4 nucleotidesPFLU_RS10270 and PFLU_RS10275 are separated by 90 nucleotides PFLU_RS31520: PFLU_RS31520 - TonB-dependent receptor, at 2,263,698 to 2,263,829 _RS31520 PFLU_RS10265: PFLU_RS10265 - LysE family translocator, at 2,263,985 to 2,264,614 _RS10265 PFLU_RS30630: PFLU_RS30630 - hypothetical protein, at 2,264,876 to 2,265,213 _RS30630 PFLU_RS10270: PFLU_RS10270 - DJ-1/PfpI family protein, at 2,265,210 to 2,265,890 _RS10270 PFLU_RS10275: PFLU_RS10275 - GlxA family transcriptional regulator, at 2,265,981 to 2,266,985 _RS10275
Group Condition PFLU_RS31520 PFLU_RS10265 PFLU_RS30630 PFLU_RS10270 PFLU_RS10275
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.2 -0.3 -4.4 -0.1 +0.0
carbon source acetate (C) -0.1 +0.0 -4.1 -0.1 -0.3
stress Betaine (C)(N); with MOPS; with chloride -0.5 -0.7 -3.7 +0.1 +0.3
stress Betaine (C)(N); with TAPS; with chloride -2.0 -0.2 -1.8 -0.3 +0.4
carbon source L-Valine 10 mM (C) -0.6 -0.2 -2.6 -0.3 -0.1
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.3 +0.4 -3.5 -0.5 +0.2
pH Growth at pH7 and (C) D-Glucose +0.0 -0.3 -2.9 -0.1 -0.5
carbon source L-Carnitine (C) +0.1 -0.4 -3.5 -0.2 +0.4
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -0.4 +0.0 -3.4 -0.0 +0.3
pH Growth at pH7 and (C) D-Glucose -0.2 +0.1 -2.9 -0.0 -0.4
carbon source 4-Aminobutyric (C) -0.1 +0.7 -4.1 -0.3 +0.3
no stress control L-Glutamine (C)(N); with TAPS -0.6 -0.5 -2.6 -0.0 +0.2
carbon source Inosine (C) -0.4 -0.0 -2.8 -0.2 +0.3
stress Glucose (C) and L-Glutamine (N); with MOPS; with chloride +0.5 +0.1 -3.6 -0.1 +0.1
no stress control Glycine betaine (C)(N); with TAPS +0.9 -0.1 -3.8 -0.5 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs +0.3 +0.1 -3.3 +0.0 -0.0
temperature Growth at 25C; with MOPS +0.0 -0.2 -3.0 -0.0 +0.3
carbon source L-Arabinose (C) +0.5 -0.1 -2.7 -0.0 -0.5
carbon source Inosine (C) +0.5 -0.4 -2.0 -0.6 -0.2
carbon source D,L-Malic Acid (C) +0.3 +0.6 -2.8 -0.2 -0.2
carbon source L-Lysine (C) -0.6 -0.5 -2.0 +0.7 +0.3
stress Betaine (C)(N); with TAPS; with chloride +0.4 -0.7 -2.0 +0.4 +0.2
carbon source 2-Deoxy-D-Ribose 10 mM (C) +0.7 -0.4 -2.2 -0.3 +0.5
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs +0.7 +0.6 -1.7 -0.4 +0.4
stress Betaine (C)(N); with MOPS; with chloride +0.8 +1.0 -2.2 +0.4 -0.0
pH Growth at pH9 and (C) Trisodium citrate -1.4 -0.4 +2.2 +0.5 -0.1
carbon source p-Coumaric (C) 5 mM +0.4 -0.6 +1.4 +0.7 -0.7
carbon source L-Valine (C) +1.0 +0.5 +0.8 -1.5 +0.4
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +1.7 +1.0 -1.7 +0.2 +0.1
carbon source p-Coumaric (C) 5 mM +1.0 -0.2 +1.7 -0.5 +0.6
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