Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS30170 and PFLU_RS30175 overlap by 4 nucleotidesPFLU_RS30175 and PFLU_RS30180 are separated by 565 nucleotidesPFLU_RS30180 and PFLU_RS30185 overlap by 1 nucleotidesPFLU_RS30185 and PFLU_RS30190 are separated by 92 nucleotides PFLU_RS30170: PFLU_RS30170 - 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG, at 6,710,863 to 6,711,507 _RS30170 PFLU_RS30175: PFLU_RS30175 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG, at 6,711,504 to 6,713,396 _RS30175 PFLU_RS30180: PFLU_RS30180 - sigma-70 family RNA polymerase sigma factor, at 6,713,962 to 6,714,468 _RS30180 PFLU_RS30185: PFLU_RS30185 - DUF4880 domain-containing protein, at 6,714,468 to 6,715,412 _RS30185 PFLU_RS30190: PFLU_RS30190 - TonB-dependent siderophore receptor, at 6,715,505 to 6,717,943 _RS30190
Group Condition PFLU_RS30170 PFLU_RS30175 PFLU_RS30180 PFLU_RS30185 PFLU_RS30190
carbon source acetate 30 mM (C) -2.3 N.D. +0.0 -0.1 -0.2
carbon source 4-Hydroxyphenylpropionic (C) 1.5 mM -2.5 N.D. -0.1 +0.2 -0.0
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -1.2 N.D. -0.6 -0.6 +0.1
carbon source 4-Hydroxyphenylpropionic (C) 1.5 mM -2.2 N.D. +0.0 -0.0 -0.0
carbon source D,L-Malic Acid (C) -1.7 N.D. +0.1 -0.1 -0.2
no stress control L-Glutamine (C)(N); with MOPS -1.0 N.D. +0.0 -0.9 -0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -1.4 N.D. -0.2 -0.3 +0.0
carbon source 4-Hydroxyphenylpropionic (C) 1.5 mM -1.9 N.D. -0.1 +0.1 -0.1
carbon source 4-Hydroxyphenylpropionic (C) 1.5 mM -1.9 N.D. -0.0 +0.1 -0.1
carbon source Shikimic Acid (C) -1.3 N.D. -0.5 -0.0 -0.0
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS -1.6 N.D. -0.2 -0.0 -0.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -1.1 N.D. -0.2 -0.4 -0.1
carbon source L-Valine 10 mM (C) -1.7 N.D. +0.1 +0.0 -0.3
carbon source propionate 20 mM (C) -1.3 N.D. +0.2 -0.5 -0.2
carbon source propionate 20 mM (C) -1.7 N.D. +0.1 +0.1 -0.2
stress Betaine (C)(N); with MOPS; with chloride -1.5 N.D. -0.3 +0.2 +0.1
stress Glucose (C) and Betaine (N); with MOPS; with chloride -1.5 N.D. -0.4 +0.4 +0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.5 N.D. -0.0 +0.2 +0.1
carbon source L-Valine 10 mM (C) -1.7 N.D. -0.1 +0.4 +0.1
carbon source 4-Aminobutyric (C) -0.9 N.D. -0.9 +0.4 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -1.7 N.D. +0.1 +0.2 +0.1
stress Betaine (C)(N); with TAPS; with chloride -1.7 N.D. +0.3 +0.2 +0.0
no stress control Glucose (C) and nitrate (N); with TAPS -1.3 N.D. +0.3 -0.2 +0.0
carbon source Quinic Acid (C) -1.1 N.D. -0.3 +0.5 -0.1
carbon source 2-Deoxy-D-Ribose 10 mM (C) -1.0 N.D. +0.5 -0.2 -0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -1.3 N.D. +0.5 +0.0 +0.0
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -1.2 N.D. +0.4 +0.5 -0.0
carbon source L-Valine (C) +1.0 N.D. -0.6 -0.5 -0.1
carbon source Choline chloride 10 mM (C) -1.1 N.D. +0.2 +0.6 +0.1
carbon source Xanthosine (C) +1.3 N.D. -0.6 +0.3 -0.1
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