Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS29955 and PFLU_RS29960 are separated by 127 nucleotidesPFLU_RS29960 and PFLU_RS29965 overlap by 4 nucleotidesPFLU_RS29965 and PFLU_RS29970 are separated by 127 nucleotidesPFLU_RS29970 and PFLU_RS29975 overlap by 4 nucleotides PFLU_RS29955: PFLU_RS29955 - RNA polymerase-binding protein DksA, at 6,663,648 to 6,664,052 _RS29955 PFLU_RS29960: PFLU_RS29960 - hypothetical protein, at 6,664,180 to 6,664,548 _RS29960 PFLU_RS29965: PFLU_RS29965 - N-acetylmuramoyl-L-alanine amidase, at 6,664,545 to 6,665,747 _RS29965 PFLU_RS29970: PFLU_RS29970 - GTP cyclohydrolase I FolE2, at 6,665,875 to 6,666,771 _RS29970 PFLU_RS29975: PFLU_RS29975 - ATP-binding cassette domain-containing protein, at 6,666,768 to 6,667,436 _RS29975
Group Condition PFLU_RS29955 PFLU_RS29960 PFLU_RS29965 PFLU_RS29970 PFLU_RS29975
carbon source 4-Aminobutyric (C) -0.4 -1.0 +0.2 -0.1 -5.2
carbon source Inosine (C) -0.9 -0.1 +0.0 -0.4 -2.5
nitrogen source Ammonium chloride (N); with MOPS -0.2 -0.1 -0.1 -0.2 -2.4
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -2.0 +0.5 -0.6 +0.0 -1.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.4 -0.0 +0.4 -0.2 -2.7
carbon source Ferulic Acid 2 mM (C) -0.1 +0.4 -0.3 -0.1 -2.4
carbon source L-Ornithine 10 mM (C) +0.8 -0.4 -0.7 -0.0 -2.1
stress malate (C) and Ammonium chloride (N); with TAPS -0.8 +0.2 -0.0 +0.1 -1.9
pH Growth at pH9 and (C) D-Glucose -1.1 +0.3 +0.3 -0.1 -1.6
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS -1.7 +0.1 -0.7 +0.2 -0.1
stress Glucose (C) and Betaine (N); with TAPS; with chloride +0.6 -0.1 -0.7 -0.3 -1.6
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.3 +0.2 -0.4 -0.0 -2.0
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.1 -0.1 +0.3 +0.3 -2.4
carbon source 2-Deoxy-D-Ribose 10 mM (C) +0.7 -0.3 -0.6 +0.1 -1.6
carbon source Shikimic Acid (C) +0.7 +0.0 -0.0 -0.1 -2.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -1.5 +0.5 +0.4 +0.2 -1.1
stress Betaine (C)(N); with TAPS; with chloride -1.2 +0.2 +0.4 +0.6 -1.4
nitrogen source NAG (N); with MOPS -1.5 -0.3 -0.1 -0.3 +1.0
pH Growth at pH8 and (C) Trisodium citrate +0.9 +0.6 -0.3 -0.2 -1.9
carbon source 4-hydroxyphenylacetic 2 mM (C) +0.6 +0.3 +0.2 -0.1 -1.8
temperature Growth at 25C; with MOPS +1.1 -0.2 -0.3 +0.1 -1.1
carbon source propionate 20 mM (C) -1.0 -0.5 -0.1 -0.1 +1.4
pH Growth at pH9 and (C) Trisodium citrate -0.6 +0.4 -0.7 -0.1 +1.5
stress Betaine (C)(N); with MOPS; with chloride -0.8 -0.5 +0.4 -0.1 +1.4
stress Betaine (C)(N); with MOPS; with chloride -0.4 +0.2 -0.3 -0.5 +1.6
stress Betaine (C)(N); with MOPS; with chloride +1.1 +0.1 +0.4 -0.1 +1.3
carbon source p-Coumaric (C) 5 mM -0.0 -0.0 +1.1 +1.2 +0.7
carbon source p-Coumaric (C) 5 mM -0.2 -0.3 +1.2 +1.4 +0.8
carbon source p-Coumaric (C) 5 mM -0.1 +0.3 +1.3 +1.4 +0.6
carbon source p-Coumaric (C) 5 mM -0.0 +0.3 +1.3 +1.2 +0.8
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