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Fitness for 5 genes in
Pseudomonas fluorescens SBW25-INTG
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
PFLU_RS29240 and PFLU_RS29245 are separated by 28 nucleotides
PFLU_RS29245 and PFLU_RS29250 are separated by 131 nucleotides
PFLU_RS29250 and PFLU_RS29255 are separated by 2 nucleotides
PFLU_RS29255 and PFLU_RS31215 are separated by 238 nucleotides
PFLU_RS29240: PFLU_RS29240 - class I adenylate cyclase, at 6,497,609 to 6,500,449
_RS29240
PFLU_RS29245: PFLU_RS29245 - nucleoside diphosphate kinase regulator, at 6,500,478 to 6,500,888
_RS29245
PFLU_RS29250: PFLU_RS29250 - DUF1289 domain-containing protein, at 6,501,020 to 6,501,238
_RS29250
PFLU_RS29255: PFLU_RS29255 - iron donor protein CyaY, at 6,501,241 to 6,501,573
_RS29255
PFLU_RS31215: PFLU_RS31215 - hypothetical protein, at 6,501,812 to 6,501,982
_RS31215
Group
Condition
PFLU
_RS29240
PFLU
_RS29245
PFLU
_RS29250
PFLU
_RS29255
PFLU
_RS31215
stress
Betaine (C)(N); with TAPS; with chloride
-0.1
-0.0
-1.5
-0.5
-3.4
stress
Betaine (C)(N); with MOPS; with chloride
-0.1
+0.2
-0.3
-1.5
-2.8
stress
Glucose (C) and Ammonium chloride (N); with MOPS; with chloride
-0.1
-0.1
-0.5
-2.1
-1.7
no stress control
Glycine betaine (C)(N); with MOPS
+0.0
-0.4
-1.1
-0.1
-3.0
stress
p-Coumaric (C) and Ammonium chloride (N); with TAPS
-0.1
-0.6
-0.8
-0.1
-2.8
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-0.0
-0.6
-0.8
-0.6
-2.3
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-0.1
-0.1
+0.8
-0.1
-4.8
stress
p-Coumaric (C) and Ammonium chloride (N); with TAPS
+0.0
-0.4
-0.5
-0.9
-2.2
no stress control
Glycine betaine (C)(N); with TAPS
-0.2
-0.6
-0.8
-1.0
-1.4
stress
Betaine (C)(N); with MOPS; with chloride
-0.3
+0.0
-2.0
-1.1
-0.6
carbon source
Betaine 10 mM (C)
+0.0
-0.0
-1.3
-2.4
-0.0
no stress control
Betaine (C)(N); with TAPS
+0.1
+0.0
-0.4
-2.5
-0.7
carbon source
propionate 20 mM (C)
-0.1
-0.5
-0.3
-2.7
+0.3
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride
+0.2
+0.0
+0.1
+0.2
-3.8
carbon source
Shikimic Acid (C)
-0.1
+0.2
-0.0
-1.3
-2.0
stress
Betaine (C)(N); with MOPS; with chloride
-0.1
+0.3
-1.3
+0.0
-2.3
stress
p-Coumaric (C) and Ammonium chloride (N); with TAPS
-0.2
-0.8
+0.4
+1.0
-3.5
carbon source
L-Valine 10 mM (C)
-0.0
+0.1
+0.5
-3.0
-0.6
stress
Glucose (C) and Ammonium chloride (N); with MOPS; with chloride
+0.2
-0.1
-3.1
+0.9
-0.8
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride
+0.0
-0.8
-1.4
+0.5
-1.2
carbon source
acetate 30 mM (C)
-0.0
+0.6
+0.4
-2.3
-1.2
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
+0.1
-0.3
+0.6
-0.5
-2.4
carbon source
L-Carnitine (C)
-0.1
-0.5
+0.4
-3.0
+0.8
temperature
Growth at 25C
-0.1
-0.4
+0.5
+0.5
-2.5
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-0.2
+0.3
+0.3
+0.4
-2.8
nitrogen source
nitrate (N); with MOPS
-0.0
+0.0
+0.3
+0.5
-2.7
carbon source
p-Coumaric (C) 5 mM
-0.0
+0.5
-0.1
+0.5
-2.6
carbon source
acetate 30 mM (C)
-0.2
-0.3
+0.6
-1.6
+0.9
no stress control
p-Coumaric (C) and Ammonium chloride (N); with MOPS
+0.0
+0.4
-1.1
+1.4
-1.1
carbon source
Inosine (C)
+0.1
-0.7
-1.6
+0.5
+2.0
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