Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS29070 and PFLU_RS29075 are separated by 37 nucleotidesPFLU_RS29075 and PFLU_RS29080 are separated by 136 nucleotidesPFLU_RS29080 and PFLU_RS29085 are separated by 133 nucleotidesPFLU_RS29085 and PFLU_RS29090 are separated by 365 nucleotides PFLU_RS29070: PFLU_RS29070 - hypothetical protein, at 6,462,754 to 6,463,254 _RS29070 PFLU_RS29075: PFLU_RS29075 - fimbria/pilus outer membrane usher protein, at 6,463,292 to 6,465,679 _RS29075 PFLU_RS29080: PFLU_RS29080 - fimbria/pilus periplasmic chaperone, at 6,465,816 to 6,466,514 _RS29080 PFLU_RS29085: PFLU_RS29085 - DUF1120 domain-containing protein, at 6,466,648 to 6,467,298 _RS29085 PFLU_RS29090: PFLU_RS29090 - exopolyphosphatase, at 6,467,664 to 6,469,244 _RS29090
Group Condition PFLU_RS29070 PFLU_RS29075 PFLU_RS29080 PFLU_RS29085 PFLU_RS29090
temperature Growth at 25C -0.2 +0.0 -0.4 -0.2 -2.3
pH Growth at pH6 and (C) D-Glucose +0.0 -0.2 -0.3 -0.1 -2.2
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.1 -0.1 -0.1 -0.0 -2.5
temperature Growth at 25C; with MOPS -1.3 +0.1 -0.2 +0.0 -1.1
nitrogen source NAG (N); with MOPS -1.6 +0.1 -0.2 +0.0 -1.0
temperature Growth at 25C -0.4 -0.0 -0.4 -0.0 -1.6
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS -1.1 +0.1 -0.0 +0.2 -1.4
temperature shift Temperature shift 30_to_25; with MOPS -0.1 -0.1 -0.3 +0.2 -1.8
pH Growth at pH6 and (C) D-Glucose +0.4 -0.1 -0.1 -0.0 -2.3
temperature Growth at 10C +0.2 -0.0 -0.0 -0.0 -2.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.2 +0.1 -0.3 +0.3 -2.2
nitrogen source no extra (N) +0.1 -0.1 -0.1 +0.1 -1.9
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=144_hrs -0.3 +0.0 +0.2 -0.1 -1.7
stress Glucose (C) and Glycine betaine (N); with TAPS; with chloride +0.2 +0.1 -0.2 +0.0 -1.9
stress Glucose (C) and Glycine betaine (N); with TAPS; with chloride +0.3 -0.0 -0.1 +0.2 -2.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.4 +0.1 +0.2 -0.2 -1.5
no stress control L-Glutamine (C)(N); with TAPS -0.1 -0.1 -0.0 +0.4 -1.9
no stress control Glucose (C) and Ammonium chloride (N); with TAPS -0.5 -0.2 +0.1 +0.2 -1.3
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS -0.2 -0.1 +0.2 +0.2 -1.7
no stress control Glucose (C) and L-Glutamine (N); with TAPS +0.5 -0.0 -0.3 +0.0 -1.5
stress Glucose (C) and L-Glutamine (N); with TAPS; with chloride +0.6 -0.0 -0.0 -0.0 -1.7
stress L-Glutamine (C)(N); with TAPS; with chloride +0.3 -0.0 +0.1 +0.1 -1.7
no stress control Glucose (C) and Glycine betaine (N); with TAPS +0.5 -0.1 -0.2 +0.1 -1.5
no stress control Glucose (C) and Glycine betaine (N); with TAPS +0.3 +0.1 +0.2 +0.0 -1.8
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -0.5 +0.3 +0.2 +0.3 -1.3
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.6 -0.0 +0.3 -0.6 +0.9
carbon source Citraconic 20 mM (C) -0.6 +0.1 -0.0 -0.3 +1.3
carbon source L-Valine 10 mM (C) -0.3 +0.1 -0.0 -0.4 +2.6
carbon source L-Valine 10 mM (C) +0.9 +0.0 -0.6 -0.1 +1.9
carbon source L-Valine (C) +0.8 -0.0 -0.1 -0.1 +1.6
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