Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS28715 and PFLU_RS28720 are separated by 2 nucleotidesPFLU_RS28720 and PFLU_RS28725 are separated by 131 nucleotidesPFLU_RS28725 and PFLU_RS28730 are separated by 46 nucleotidesPFLU_RS28730 and PFLU_RS28735 are separated by 99 nucleotides PFLU_RS28715: PFLU_RS28715 - DUF523 domain-containing protein, at 6,388,467 to 6,388,961 _RS28715 PFLU_RS28720: PFLU_RS28720 - 2OG-Fe(II) oxygenase, at 6,388,964 to 6,389,596 _RS28720 PFLU_RS28725: PFLU_RS28725 - DUF2059 domain-containing protein, at 6,389,728 to 6,390,480 _RS28725 PFLU_RS28730: PFLU_RS28730 - alpha/beta hydrolase, at 6,390,527 to 6,391,468 _RS28730 PFLU_RS28735: PFLU_RS28735 - thiol-disulfide isomerase, at 6,391,568 to 6,392,143 _RS28735
Group Condition PFLU_RS28715 PFLU_RS28720 PFLU_RS28725 PFLU_RS28730 PFLU_RS28735
carbon source NAG (C) -0.3 +0.4 -3.5 +0.0 -0.4
carbon and nitrogen source NAG carbon and (N) -0.4 +0.6 -3.7 -0.1 +0.0
carbon source acetate (C) -0.3 +0.4 -3.2 -0.3 -0.0
carbon source acetate (C) -0.0 +0.3 -3.5 -0.2 +0.1
pH Growth at pH9 and (C) Trisodium citrate +0.2 +0.4 -2.8 -0.7 -0.2
carbon source acetate 30 mM (C) -0.1 +0.5 -3.0 -0.4 -0.1
carbon source L-Arabinose (C); with MOPS -0.4 +0.1 -2.8 -0.1 +0.3
carbon and nitrogen source NAG carbon and (N) -0.1 +0.7 -2.9 +0.0 -0.7
carbon source acetate (C) +0.2 +0.3 -3.0 -0.3 -0.1
carbon source L-Arabinose (C) +0.0 +0.3 -2.8 +0.0 -0.3
carbon source acetate 30 mM (C) +0.2 +0.5 -3.0 -0.1 -0.3
carbon source NAG (C) +0.0 +0.6 -3.9 +0.3 +0.3
pH Growth at pH8 and (C) Trisodium citrate +0.5 +0.7 -2.3 -0.7 -0.5
pH Growth at pH9 and (C) D-Glucose +0.3 +0.7 -3.6 +0.2 -0.0
carbon and nitrogen source NAG carbon and (N); with MOPS -0.2 +0.4 -2.4 -0.0 -0.2
carbon and nitrogen source NAG carbon and (N); with MOPS -0.5 +0.3 -2.6 +0.1 +0.3
carbon source NAG (C); with MOPS -0.3 +0.2 -2.5 -0.1 +0.4
temperature shift Temperature shift 30_to_34 +0.2 -0.2 -2.7 +0.0 +0.3
carbon source NAG (C) -0.4 +0.7 -2.8 +0.1 +0.2
carbon and nitrogen source NAG carbon and (N); with MOPS +0.2 +0.4 -2.9 +0.1 -0.0
nitrogen source no extra (N) +0.0 +0.4 -2.9 +0.2 +0.2
temperature Growth at 25C -1.4 +0.2 -1.2 +0.0 +0.4
carbon source L-Arabinose (C) -0.3 +0.3 -2.3 +0.3 +0.0
carbon source propionate 20 mM (C) -0.3 +0.6 -2.4 +0.2 -0.1
pH Growth at pH9 and (C) Trisodium citrate -0.0 +0.6 -2.6 -0.2 +0.2
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs -0.3 -0.2 +1.0 -0.0 -2.2
carbon and nitrogen source NAG carbon and (N) -0.1 +0.6 -3.0 +0.4 +0.3
nitrogen source no extra (N) -0.1 +0.4 -2.3 +0.3 +0.3
carbon source propionate 20 mM (C) +0.2 +0.6 -1.7 +0.3 -0.3
carbon source p-Coumaric (C) 5 mM -0.1 -0.2 +2.2 +0.2 +0.9
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