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Fitness for 5 genes in
Pseudomonas fluorescens SBW25-INTG
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
PFLU_RS28470 and PFLU_RS28475 are separated by 179 nucleotides
PFLU_RS28475 and PFLU_RS28480 overlap by 14 nucleotides
PFLU_RS28480 and PFLU_RS28485 are separated by 33 nucleotides
PFLU_RS28485 and PFLU_RS28490 are separated by 146 nucleotides
PFLU_RS28470: PFLU_RS28470 - 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD, at 6,334,314 to 6,334,931
_RS28470
PFLU_RS28475: PFLU_RS28475 - hydrolase, at 6,335,111 to 6,336,103
_RS28475
PFLU_RS28480: PFLU_RS28480 - sulfurtransferase, at 6,336,090 to 6,336,944
_RS28480
PFLU_RS28485: PFLU_RS28485 - TetR/AcrR family transcriptional regulator, at 6,336,978 to 6,337,649
_RS28485
PFLU_RS28490: PFLU_RS28490 - coniferyl aldehyde dehydrogenase, at 6,337,796 to 6,339,223
_RS28490
Group
Condition
PFLU
_RS28470
PFLU
_RS28475
PFLU
_RS28480
PFLU
_RS28485
PFLU
_RS28490
stress
Glucose (C) and Ammonium chloride (N); with MOPS; with chloride
-2.7
-0.4
-0.5
-0.4
+0.0
stress
Betaine (C)(N); with TAPS; with chloride
-2.7
-0.1
-0.1
-0.9
-0.0
stress
p-Coumaric (C) and Ammonium chloride (N); with TAPS
-2.6
+0.3
-0.7
-0.4
-0.1
stress
Betaine (C)(N); with TAPS; with chloride
-2.5
-0.0
-0.3
-0.3
+0.0
stress
p-Coumaric (C) and Ammonium chloride (N); with TAPS
-3.0
+0.2
+0.0
+0.1
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-1.7
-0.2
-1.0
-0.2
+0.2
carbon source
Xanthosine (C)
-1.5
-0.0
-0.8
-0.2
-0.1
stress
L-Glutamine (C)(N); with MOPS; with chloride
-1.9
-0.1
-0.6
-0.1
+0.1
stress
Betaine (C)(N); with TAPS; with chloride
-2.1
-0.0
-0.1
-0.2
-0.1
stress
p-Coumaric (C) and Ammonium chloride (N); with TAPS
-2.6
-0.3
+0.0
+0.0
+0.3
stress
Glucose (C) and Betaine (N); with TAPS; with chloride
-2.3
+0.1
-0.2
-0.2
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-2.4
+0.1
+0.1
-0.2
-0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs
-2.7
-0.1
+0.2
-0.1
+0.2
carbon source
acetate 30 mM (C)
-2.2
-0.2
-0.0
-0.2
+0.1
stress
p-Coumaric (C) and Ammonium chloride (N); with TAPS
-2.3
-0.2
+0.1
+0.1
-0.2
stress
Glucose (C) and Ammonium chloride (N); with MOPS; with chloride
-1.8
+0.1
-0.7
+0.1
-0.1
carbon source
4-hydroxyphenylacetic 2 mM (C)
-1.8
+0.1
-0.3
-0.2
-0.2
stress
D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride
-1.5
+0.1
-0.5
-0.5
+0.2
carbon source
p-Coumaric 2 mM (C)
-1.2
-0.4
-0.7
-0.2
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-2.6
+0.1
+0.1
+0.2
-0.0
no stress control
Glycine betaine (C)(N); with TAPS
-2.0
+0.1
+0.2
-0.4
-0.0
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs
-2.1
+0.1
-0.1
+0.1
-0.0
stress
p-Coumaric (C) and Ammonium chloride (N); with TAPS
-1.8
-0.2
-0.3
+0.2
+0.1
stress
Glucose (C) and Ammonium chloride (N); with MOPS; with chloride
-2.2
-0.1
+0.3
-0.1
+0.0
stress
Glucose (C) and Ammonium chloride (N); with MOPS; with chloride
-2.1
+0.1
-0.2
+0.1
+0.2
stress
Betaine (C)(N); with TAPS; with chloride
-1.7
-0.2
+0.2
-0.3
+0.2
carbon source
acetate 30 mM (C)
-2.0
-0.2
+0.5
-0.3
+0.3
stress
L-Glutamine (C)(N); with TAPS; with chloride
-2.3
+0.1
+0.3
+0.2
+0.1
soil
Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs
-1.4
+0.3
+0.4
+0.2
+0.3
carbon source
p-Coumaric (C) 5 mM
+2.2
-0.0
+0.2
-0.2
+0.1
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