Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS28130 and PFLU_RS28135 are separated by 230 nucleotidesPFLU_RS28135 and PFLU_RS28140 are separated by 205 nucleotidesPFLU_RS28140 and PFLU_RS28145 overlap by 7 nucleotidesPFLU_RS28145 and PFLU_RS28150 are separated by 71 nucleotides PFLU_RS28130: PFLU_RS28130 - murein transglycosylase A, at 6,263,837 to 6,265,021 _RS28130 PFLU_RS28135: PFLU_RS28135 - membrane protein, at 6,265,252 to 6,265,638 _RS28135 PFLU_RS28140: PFLU_RS28140 - sodium:proton antiporter, at 6,265,844 to 6,267,103 _RS28140 PFLU_RS28145: PFLU_RS28145 - formate transporter, at 6,267,097 to 6,267,984 _RS28145 PFLU_RS28150: PFLU_RS28150 - hypothetical protein, at 6,268,056 to 6,269,987 _RS28150
Group Condition PFLU_RS28130 PFLU_RS28135 PFLU_RS28140 PFLU_RS28145 PFLU_RS28150
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS -0.3 -0.4 -0.3 +0.2 -0.2
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.1 -0.6 -0.4 +0.3 -0.2
carbon source L-tyrosine 10 mM (C) -0.6 +0.1 -0.1 -0.3 -0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.2 -0.6 +0.3 -0.2 -0.1
temperature Growth at 25C -0.2 -0.1 -0.3 +0.1 -0.3
carbon source D-Glucosamine Hydrochloride 10 mM (C) +0.1 -0.8 -0.3 +0.0 +0.2
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.3 -0.3 -0.3 -0.4 +0.0
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.2 -0.5 -0.2 -0.2 -0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.2 -0.6 +0.2 -0.3 +0.3
stress Betaine (C)(N); with MOPS; with chloride -0.3 -0.4 +0.4 -0.1 +0.0
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.1 -0.7 +0.5 -0.2 +0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -0.2 -0.5 +0.1 +0.1 +0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.3 -0.4 +0.3 +0.2 -0.0
carbon source propionate 20 mM (C) +0.2 -0.3 +0.3 -0.3 -0.1
stress Betaine (C)(N); with TAPS; with chloride +0.2 -0.4 +0.2 -0.2 +0.2
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.4 -0.1 +0.4 +0.3 -0.1
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.2 -0.4 -0.1 +0.5 +0.3
pH Growth at pH9 and (C) Trisodium citrate +0.2 -0.3 -0.2 +0.4 +0.1
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.0 +0.4 -0.4 +0.3 -0.1
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.2 +0.1 +0.3 +0.4 -0.2
carbon source L-Valine (C) +0.4 -0.1 +0.1 +0.3 -0.2
carbon source NAG (C) +0.3 -0.2 -0.2 +0.4 +0.2
carbon source L-Carnitine (C) +0.2 +0.2 +0.3 -0.4 +0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride +0.4 +0.4 -0.2 +0.1 -0.1
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.3 -0.4 +0.2 +0.2 +0.2
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.1 +0.5 +0.2 +0.1 -0.2
stress Betaine (C)(N); with MOPS; with chloride +0.1 +0.6 +0.1 +0.3 -0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs +0.2 +0.6 +0.1 +0.1 -0.0
carbon source 4-Guanidinobutyric (C) +0.3 -0.0 +0.3 -0.0 +0.6
carbon source Xanthosine (C) +0.1 +0.3 +0.1 +0.0 +0.6
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