Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS28100 and PFLU_RS28105 are separated by 19 nucleotidesPFLU_RS28105 and PFLU_RS28110 are separated by 222 nucleotidesPFLU_RS28110 and PFLU_RS28115 are separated by 95 nucleotidesPFLU_RS28115 and PFLU_RS28120 are separated by 42 nucleotides PFLU_RS28100: PFLU_RS28100 - metalloregulator ArsR/SmtB family transcription factor, at 6,258,275 to 6,259,270 _RS28100 PFLU_RS28105: PFLU_RS28105 - methionine adenosyltransferase, at 6,259,290 to 6,260,480 _RS28105 PFLU_RS28110: PFLU_RS28110 - hypothetical protein, at 6,260,703 to 6,261,053 _RS28110 PFLU_RS28115: PFLU_RS28115 - NAD-dependent DNA ligase LigB, at 6,261,149 to 6,262,813 _RS28115 PFLU_RS28120: PFLU_RS28120 - DUF1090 domain-containing protein, at 6,262,856 to 6,263,251 _RS28120
Group Condition PFLU_RS28100 PFLU_RS28105 PFLU_RS28110 PFLU_RS28115 PFLU_RS28120
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.0 N.D. -1.9 -0.2 -2.0
carbon source Shikimic Acid (C) -0.3 N.D. -2.0 +0.0 -0.9
carbon source 4-Aminobutyric (C) -0.5 N.D. -2.8 -0.1 +0.6
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.2 N.D. -1.4 -0.2 -0.9
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.1 N.D. -0.1 -0.1 -2.0
carbon source 2-Deoxy-D-Ribose 10 mM (C) -0.3 N.D. -0.6 -0.2 -1.1
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS +0.1 N.D. -1.3 +0.0 -1.0
no stress control p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.2 N.D. +0.2 -0.0 -2.1
carbon source L-Valine (C) -0.5 N.D. -0.2 -0.5 -0.9
pH Growth at pH8 and (C) D-Glucose -0.2 N.D. -0.4 -0.3 -1.3
no stress control L-Glutamine (C)(N); with MOPS -0.3 N.D. -1.3 -0.2 -0.3
carbon source Inosine (C) -0.6 N.D. +0.4 +0.0 -1.8
no stress control L-Glutamine (C)(N); with TAPS -0.2 N.D. +0.1 -0.0 -1.8
carbon source propionate 20 mM (C) +0.2 N.D. -2.1 -0.1 +0.2
stress D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride -0.5 N.D. -1.0 +0.2 -0.6
no stress control D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS +0.2 N.D. -1.2 +0.1 -0.9
carbon source propionate 20 mM (C) -0.7 N.D. +0.1 +0.3 -1.3
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.9 N.D. -0.5 -0.4 +0.4
carbon source m-Inositol (C) +0.3 N.D. +0.3 +0.0 -1.8
carbon source p-Coumaric 2 mM (C) -0.1 N.D. +0.6 +0.1 -1.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs -0.3 N.D. -1.3 +0.0 +0.5
carbon source Phloretic Acid 2 mM (C) +0.3 N.D. -1.6 -0.1 +0.5
carbon source NAG (C) -0.6 N.D. +1.0 -0.1 -1.0
carbon source NAG (C) -0.9 N.D. -0.4 -0.0 +0.7
no stress control Glycine betaine (C)(N); with TAPS +0.4 N.D. -1.6 +0.2 +0.7
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.2 N.D. +0.3 -0.2 +1.0
pH Growth at pH9 and (C) D-Glucose -0.2 N.D. +1.4 +0.1 -1.2
stress Betaine (C)(N); with TAPS; with chloride +0.3 N.D. -0.9 -0.1 +0.9
carbon source Shikimic Acid (C) +0.4 N.D. +0.8 -0.3 +0.6
stress Betaine (C)(N); with TAPS; with chloride +0.0 N.D. +0.6 +0.3 +1.3
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