Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS27995 and PFLU_RS28000 are separated by 191 nucleotidesPFLU_RS28000 and PFLU_RS28005 overlap by 1 nucleotidesPFLU_RS28005 and PFLU_RS28010 are separated by 224 nucleotidesPFLU_RS28010 and PFLU_RS28015 are separated by 36 nucleotides PFLU_RS27995: PFLU_RS27995 - choline dehydrogenase, at 6,232,822 to 6,234,525 _RS27995 PFLU_RS28000: PFLU_RS28000 - TldD/PmbA family protein, at 6,234,717 to 6,236,159 _RS28000 PFLU_RS28005: PFLU_RS28005 - TldD/PmbA family protein, at 6,236,159 to 6,237,475 _RS28005 PFLU_RS28010: PFLU_RS28010 - alpha/beta hydrolase, at 6,237,700 to 6,238,590 _RS28010 PFLU_RS28015: PFLU_RS28015 - DHA2 family efflux MFS transporter permease subunit, at 6,238,627 to 6,240,054 _RS28015
Group Condition PFLU_RS27995 PFLU_RS28000 PFLU_RS28005 PFLU_RS28010 PFLU_RS28015
carbon source sn-glycero-3-phosphocholine 10 mM (C) -4.4 -0.1 -0.4 +0.1 -0.1
carbon source sn-glycero-3-phosphocholine 10 mM (C) -4.2 -0.6 -0.5 +0.4 +0.2
carbon source Choline chloride 10 mM (C) -3.8 -0.2 -0.2 +0.1 -0.0
carbon source Choline chloride 10 mM (C) -3.0 +0.0 -0.8 -0.2 -0.0
carbon source Choline chloride 10 mM (C) -3.9 -0.2 -0.1 +0.1 +0.1
carbon source sn-glycero-3-phosphocholine 10 mM (C) -3.8 -0.1 -0.2 +0.1 +0.1
carbon source sn-glycero-3-phosphocholine (C) -3.2 +0.1 +0.1 -0.1 -0.1
carbon source sn-glycero-3-phosphocholine (C) -2.9 -0.2 +0.1 +0.0 -0.0
carbon source Choline chloride (C) -2.9 -0.2 +0.4 -0.2 -0.1
carbon source sn-glycero-3-phosphocholine (C) -3.3 +0.1 +0.2 +0.0 +0.0
carbon source Choline chloride (C) -2.2 +0.2 +0.1 +0.0 -0.0
carbon source Choline chloride (C) -1.8 +0.4 -0.0 +0.1 +0.1
carbon source Inosine (C) -0.8 -0.1 -0.2 +0.2 -0.3
stress Betaine (C)(N); with MOPS; with chloride +0.3 -0.3 -0.8 +0.3 -0.8
carbon source propionate 20 mM (C) +0.1 -0.6 -0.8 +0.3 +0.0
pH Growth at pH9 and (C) Trisodium citrate -0.2 -0.3 +0.4 -0.2 -0.7
no stress control Glycine betaine (C)(N); with TAPS -1.3 +0.5 -0.3 +0.1 +0.2
no stress control Glycine betaine (C)(N); with TAPS -1.1 +0.1 +0.1 -0.1 +0.4
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS +0.3 -0.4 +0.2 +0.2 -0.6
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride -0.5 -0.1 +0.2 -0.3 +0.4
carbon source caffeic 2 mM (C) +0.1 +0.2 +0.4 -0.2 -0.8
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.5 -0.9 -0.2 +0.2 +0.3
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS +0.8 -0.2 -0.5 +0.2 -0.4
no stress control 4-Hydroxybenzoic Acid (C) and Ammonium chloride (N); with MOPS -0.8 -0.1 +0.1 +0.2 +0.5
carbon source Phloretic Acid 2 mM (C) +0.3 -0.5 +0.7 +0.0 -0.2
nitrogen source nitrate (N) -0.1 +0.3 +0.7 -0.0 -0.5
stress Betaine (C)(N); with MOPS; with chloride +0.2 -0.3 +0.5 +0.3 +0.3
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs +0.2 +0.4 +0.3 -0.2 +0.6
carbon source p-Coumaric 2 mM (C) +0.3 +0.7 +0.6 +0.5 -0.3
stress Betaine (C)(N); with MOPS; with chloride +0.1 +0.8 +0.2 +0.2 +0.5
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