Fitness for 5 genes in Pseudomonas fluorescens SBW25-INTG

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500 ntPFLU_RS26455 and PFLU_RS26460 are separated by 43 nucleotidesPFLU_RS26460 and PFLU_RS26465 are separated by 117 nucleotidesPFLU_RS26465 and PFLU_RS26470 are separated by 176 nucleotidesPFLU_RS26470 and PFLU_RS26475 are separated by 40 nucleotides PFLU_RS26455: PFLU_RS26455 - 3'-5' exonuclease, at 5,914,858 to 5,915,469 _RS26455 PFLU_RS26460: PFLU_RS26460 - NYN domain-containing protein, at 5,915,513 to 5,915,989 _RS26460 PFLU_RS26465: PFLU_RS26465 - DUF2076 domain-containing protein, at 5,916,107 to 5,916,853 _RS26465 PFLU_RS26470: PFLU_RS26470 - membrane protein, at 5,917,030 to 5,917,689 _RS26470 PFLU_RS26475: PFLU_RS26475 - endonuclease/exonuclease/phosphatase family protein, at 5,917,730 to 5,918,818 _RS26475
Group Condition PFLU_RS26455 PFLU_RS26460 PFLU_RS26465 PFLU_RS26470 PFLU_RS26475
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.9 -0.0 -0.6 -0.4 -0.3
carbon source Shikimic Acid (C) -0.3 -0.7 -0.0 -0.1 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs -1.0 -0.7 -0.2 +0.2 +0.0
no stress control Glycine betaine (C)(N); with TAPS -1.1 +0.1 -0.5 -0.0 -0.1
carbon source Ferulic Acid 2 mM (C) -1.3 -0.2 -0.1 -0.1 +0.2
carbon source Choline chloride 10 mM (C) -1.0 -0.2 -0.1 +0.1 -0.3
carbon source D-Xylose (C) -1.2 +0.1 -0.1 -0.1 -0.2
stress p-Coumaric (C) and Ammonium chloride (N); with TAPS -0.7 -0.9 +0.2 -0.1 -0.0
nitrogen source Ammonium chloride (N); with MOPS -1.3 -0.4 -0.1 +0.0 +0.3
carbon source Phloretic Acid 2 mM (C) -1.4 +0.2 +0.1 -0.1 -0.1
carbon source 2-Deoxy-D-Ribose 10 mM (C) -1.1 +0.1 -0.3 -0.1 +0.1
no stress control p-Coumaric (C) and Ammonium chloride (N); with MOPS -0.8 -0.4 -0.1 -0.1 +0.4
no stress control L-Glutamine (C)(N); with MOPS -0.4 -0.6 +0.3 -0.2 -0.1
carbon source p-Coumaric (C) 5 mM -1.2 +0.4 -0.0 +0.1 -0.1
stress L-Glutamine (C)(N); with MOPS; with chloride -1.1 +0.2 +0.1 -0.1 +0.1
no stress control Glycine betaine (C)(N); with MOPS -0.7 -0.3 +0.0 -0.1 +0.4
carbon source 4-Aminobutyric (C) -0.8 +0.5 +0.3 -0.1 -0.3
pH Growth at pH9 and (C) Trisodium citrate -1.0 +0.8 -0.2 +0.0 +0.2
carbon source Inosine (C) -0.2 +0.4 +0.3 +0.0 -0.6
soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=none; SoilTreatmentTime=288_hrs -0.6 +0.4 -0.2 +0.3 +0.2
carbon source L-Valine (C) -1.2 +0.2 +0.3 +0.3 +0.5
carbon source L-Valine (C) -0.8 +0.4 +0.0 +0.1 +0.3
carbon source L-Carnitine (C) -0.3 +0.6 -0.6 +0.3 +0.4
carbon source 2-Deoxy-D-Ribose 10 mM (C) -0.6 +0.4 +0.3 +0.1 +0.4
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride -0.6 +0.3 +0.6 +0.2 +0.1
stress Glucose (C) and Ammonium chloride (N); with MOPS; with chloride +0.3 +0.4 -0.4 +0.6 +0.3
carbon source Xanthosine (C) +0.6 +0.3 +0.6 -0.1 -0.0
carbon source 4-Guanidinobutyric (C) +0.5 +0.4 +0.7 -0.1 +0.0
stress D,L-Malic Acid (C) and Ammonium chloride (N); with TAPS; with chloride +0.2 +0.1 +0.7 +0.4 +0.1
pH Growth at pH9 and (C) Trisodium citrate +0.2 +1.0 -0.0 +0.3 +0.4
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